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Volumn 15, Issue 7, 2002, Pages 585-593

Improving the carboligase activity of benzoylformate decarboxylase from Pseudomonas putida by a combination of directed evolution and site-directed mutagenesis

(5) Lingen, B a Grotzinger J b Kolter, D c Kula, M R a Pohl, M a

a HEINRICH HEINE UNIVERSITY (Germany)

b UNIVERSITY OF KIEL (Germany)

c FORSCHUNGSZENTRUM JÜLICH (Germany)

Author keywords

Benzoylformate decarboxylase; Directed evolution; Organic solvents; Random mutagenesis; ThDP; Total activity

Indexed keywords

2 HYDROXY 1 PHENYLPROPANONE; ACETONE; BENZOYLFORMATE DECARBOXYLASE; CARBOXYLYASE; SOLVENT; UNCLASSIFIED DRUG; WATER;

ARTICLE; CATALYSIS; CHEMICAL BOND; ENANTIOMER; ENANTIOSELECTIVITY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; EVOLUTION; MISCIBILITY; NONHUMAN; PRIORITY JOURNAL; PSEUDOMONAS PUTIDA; SITE DIRECTED MUTAGENESIS;

NEGIBACTERIA; PSEUDOMONAS; PSEUDOMONAS PUTIDA; PYRONIA TITHONUS;

EID: 0036656222 PISSN: 02692139 EISSN: None Source Type: Journal
DOI: 10.1093/protein/15.7.585 Document Type: Article

Times cited : (64)

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