Metabolic engineering of Escherichia coli using CRISPR-Cas9 meditated genome editing

Metabolic Engineering

Volumn 31, Issue , 2015, Pages 13-21

  Li, Yifan ^a,b   Lin, Zhenquan ^a,b   Huang, Can ^a,b   Zhang, Yan ^a,b   Wang, Zhiwen ^a,b   Tang, Ya jie ^c   Chen, Tao ^a,b   Zhao, Xueming ^a,b  

^a TIANJIN UNIVERSITY   (China)

^b SynBio Research Platform   (China)

^c HUBEI UNIVERSITY OF TECHNOLOGY   (China)

Author keywords

Combinatorial metabolic engineering; CRISPR Cas9; Genome editing; Carotene

Indexed keywords

ESCHERICHIA COLI; FERMENTATION; GENES; ITERATIVE METHODS; METABOLISM; MOBILE SECURITY;

BACTERIAL GENOMES; CELLULAR METABOLISM; CRISPR/CAS9; FED-BATCH FERMENTATION; METABOLIC PATHWAYS; MISMATCH REPAIR SYSTEMS; SYNTHETIC PATHWAYS; VALUABLE CHEMICALS;

METABOLIC ENGINEERING;

BETA CAROTENE; CRISPR ASSOCIATED PROTEIN; CRISPR ASSOCIATED PROTEIN 9; METHYLERYTHRITOL PHOSPHATE; PHOSPHATE; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL CELL; BACTERIAL GENOME; BIOSYNTHESIS; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; CONTROLLED STUDY; DNA MODIFICATION; ESCHERICHIA COLI; FED BATCH FERMENTATION; FERMENTATION OPTIMIZATION; GENE CONSTRUCT; GENE MUTATION; GENETIC ASSOCIATION; GENETIC ENGINEERING; GENETIC RECOMBINATION; GENETIC VARIABILITY; GENOME; GENOME EDITING; METABOLIC ENGINEERING; MISMATCH REPAIR; NONHUMAN; PRIORITY JOURNAL; SIGNAL TRANSDUCTION; CRISPR CAS SYSTEM; GENETICS; METABOLISM; RNA EDITING;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

BETA CAROTENE; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEATS; CRISPR-CAS SYSTEMS; DNA MISMATCH REPAIR; ESCHERICHIA COLI; GENOME, BACTERIAL; METABOLIC ENGINEERING; RNA EDITING;

EID: 84937538704     PISSN: 10967176     EISSN: 10967184     Source Type: Journal    
DOI: 10.1016/j.ymben.2015.06.006     Document Type: Article

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