In Silico Meets In Vivo: Towards Computational CRISPR-Based sgRNA Design

Trends in Biotechnology

Volumn 35, Issue 1, 2017, Pages 12-21

  Chuai, Guo hui ^a   Wang, Qi Long ^b   Liu, Qi ^a  

^a TONGJI UNIVERSITY   (China)

^b NANJING MEDICAL UNIVERSITY   (China)

Author keywords

CRISPR; genome editing; in silico sgRNA design; off target

Indexed keywords

BENCHMARKING; BIOINFORMATICS; GENE EXPRESSION; GENE THERAPY; RNA;

APPLICATION SCENARIO; COMPREHENSIVE EVALUATION; COMPUTATIONAL PROBLEM; COMPUTATIONAL TECHNIQUE; CRISPR; IN-SILICO; IN-SILICO MODELS; RAPIDLY ADVANCING TECHNOLOGY;

GENES;

GUIDE RNA; SINGLE GUIDE RNA; UNCLASSIFIED DRUG;

BIOINFORMATICS; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; COMPUTER MODEL; DNA END JOINING REPAIR; GENE EDITING; GENE EXPRESSION; GENE INACTIVATION; GENE TARGETING; HUMAN; MOLECULAR LIBRARY; NONHUMAN; PRIORITY JOURNAL; REVIEW; ANIMAL; BIOLOGICAL MODEL; COMPUTER SIMULATION; GENETICS; HIGH THROUGHPUT SEQUENCING; PROCEDURES; RNA EDITING; SEQUENCE ANALYSIS;

ANIMALS; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEATS; COMPUTER SIMULATION; GENE EDITING; GENE TARGETING; HIGH-THROUGHPUT NUCLEOTIDE SEQUENCING; HUMANS; MODELS, GENETIC; RNA EDITING; RNA, GUIDE; SEQUENCE ANALYSIS, RNA;

EID: 84997848522     PISSN: 01677799     EISSN: 18793096     Source Type: Journal    
DOI: 10.1016/j.tibtech.2016.06.008     Document Type: Review

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