Rapid identification of essential and nonessential herpesvirus genes by direct transposon mutagenesis

Nature Biotechnology

Volumn 17, Issue 4, 1999, Pages 360-364

  Brune, Wolfram ^a   Ménard, Carine ^a   Hobom, Urs ^a   Odenbreit, Stefan ^a   Messerle, Martin ^a   Koszinowski, Ulrich H ^a  

^a UNIVERSITY OF MUNICH   (Germany)

Author keywords

BAC; Cytomegalovirus; Functional genomics; Transposon mutagenesis

Indexed keywords

VIRUS DNA;

ARTICLE; CHROMATID EXCHANGE; DNA SEQUENCE; ESCHERICHIA COLI; GENE FUNCTION; GENE INSERTION; HERPES VIRUS; LETHAL MUTANT; MUTAGENESIS; NONHUMAN; PRIORITY JOURNAL; REVERTANT; TRANSPOSON; VIRUS GENE;

BASE SEQUENCE; CHROMOSOMES, BACTERIAL; DNA TRANSPOSABLE ELEMENTS; DNA, VIRAL; GENES, ESSENTIAL; GENES, VIRAL; MOLECULAR SEQUENCE DATA; MUROMEGALOVIRUS; MUTAGENESIS, INSERTIONAL; PLASMIDS; POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS, DNA;

EID: 0345369642     PISSN: 10870156     EISSN: None     Source Type: Journal    
DOI: 10.1038/7914     Document Type: Article

References (24)

1. Chee, M.S. et al. Analysis of the protein-coding content of the sequence of human cytomegalovirus strain AD169. Curr. Top. Microbiol. Immunol. 154, 125-169 (1990).
2. Rawlinson, W.D., Farrell, H.E. & Barrell, B.G. Analysis of the complete DNA sequence of murine cytomegalovirus. J. Virol. 70, 8833-8849 (1996).
3. Mocarski, E.S. in Fields virology (eds Fields, B.N., Knipe, D.M., & Howley, P.M.) 2447-2492 (Lippincott-Raven, Philadelphia; 1996).
4. Hengel, H., Brune, W. & Koszinowski, U.H. Immune evasion by cytomegalovirus - survival strategies of a highly adapted opportunist. Trends Microbiol. 6, 190-197 (1998).
5. Glorioso, J.C., DeLuca, N.A. & Fink, D.J. Development and application of herpes simplex virus vectors for gene therapy. Annu. Rev. Microbiol. 49, 675-714 (1995).
6. Roizman, B. & Sears A.E. in Fields virology (eds Fields, B.N., Knipe, D.M. & Howley, P.M.) 2231-2296 (Lippincott-Raven, Philadelphia; 1996).
7. Mocarski, E.S. & Kemble, G.W. Recombinant cytomegaloviruses for study of replication and pathogenesis. Intervirology 39, 320-330 (1996).
8. Messerle, M., Crnkovic, I., Ziegler, H., Hammerschmidt, W. & Koszinowski, U.H. Cloning and mutagenesis of a herpesvirus genome as an infectious bacterial artificial chromosome. Proc. Natl. Acad. Sci. USA 94, 14759-14763 (1997).
9. Delecluse, H.J., Hilsendegen, T., Pich, D., Zeidler, R. & Hammerschmidt, W. Propagation and recovery of intact, infectious Epstein-Barr virus from prokaryotic to human cells. Proc. Natl. Acad. Sci. USA 95, 8245-8250 (1998).
10. Stavropoulos, T.A. & Strathdee, C.A. An enhanced packaging system for helper-dependent herpes simplex virus vectors. J. Virol. 72, 7137-7143 (1998).
11. Berg, C.M., Berg, D.E. & Groisman, E.A. in Mobile DNA (eds Berg, D.E. & Howe, M.) 879-925 (American Society of Microbiology, Washington, DC; 1989).
12. Haas, R. et al. TnMax - a versatile mini-transposon for the analysis of cloned genes and shuttle mutagenesis. Gene 130, 23-31 (1993).
13. Kahrs, A.F. et al. An improved mini-transposon system suitable for sequencing, shuttle mutagenesis and gene fusion. Gene 167, 53-57 (1995).
14. Posfai, G., Koob, M.D., Kirkpatrick, H.A. & Blattner, F.R. Versatile insertion plasmids for targeted genome manipulations in bacteria: isolation, deletion, and rescue of the pathogenicity island LEE of the Escherichia coli O157:H7 genome. J. Bacteriol. 179, 4426-4428 (1997).
15. Jenkins, F.J., Casadaban, M.J. & Roizman, B. Application of the mini-Mu-phage for target-sequence-specific insertional mutagenesis of the herpes simplex virus genome. Proc. Natl. Acad. Sci. USA 82, 4773-4777 (1985).
16. Jenkins, F.J. & Roizman, B. Herpes simplex virus 1 recombinants with noninverted genomes frozen in different isomeric arrangements are capable of independent replication. J. Virol. 59, 494-499 (1986).
17. Weber, P.C., Levine, M. & Glorioso, J.C. Rapid identification of nonessential genes of herpes simplex virus type 1 by Tn5 mutagenesis. Science 236, 576-579 (1987).
18. Sacks, W.R. & Schaffer, P.A. Deletion mutants of the herpes simplex virus type 1 immediate early protein ICP0 exhibit impaired growth in cell culture. J. Virol. 61, 829-839 (1987).
19. Martinez, R., Sarisky, R.T., Weber, P.C. & Weller, S.K. Herpes simplex virus type 1 alkaline nuclease is required for efficient processing of viral DNA replication intermediates. J. Virol. 70, 2075-2085 (1996).
20. Spaete, R.R., Gehrz, R.C. & Landini, M.P. Human cytomegalovirus structural proteins. J. Gen. Virol. 75, 3287-3308 (1994).
21. Chang, Y.E. & Roizman, B. The product of the UL31 gene of herpes simplex virus 1 is a nuclear phosphoprotein which partitions with the nuclear matrix. J. Virol. 67, 6348-6356 (1993).
22. Simon, M.I. Dysfunctional genomics: BACs to the rescue. Nat. Biotechnol. 15, 839 (1997).
23. Ebeling, A., Keil, G.M., Knust, E. & Koszinowski, U.H. Molecular cloning and physical mapping of murine cytomegalovirus DNA. J. Virol. 47, 421-433 (1983).
24. Blomfield, I.C., Vaughn, V., Rest, R.F. & Eisenstein, B.I. Allelic exchange in Escherichia coli using the Bacillus subtilis sacB gene and a temperature-sensitive pSC101 replicon. Mol. Microbiol. 5, 1447-1457 (1991).