Multiplex metabolic pathway engineering using CRISPR/Cas9 in Saccharomyces cerevisiae

Metabolic Engineering

Volumn 28, Issue , 2015, Pages 213-222

  Jakočinas, Tadas ^a   Bonde, Ida ^a   Herrgård, Markus ^a   Harrison, Scott J ^a   Kristensen, Mette ^a   Pedersen, Lasse E ^a   Jensen, Michael K ^a   Keasling, Jay D ^a,b,c,d  

^a TECHNICAL UNIVERSITY OF DENMARK   (Denmark)

^b JOINT BIOENERGY INSTITUTE   (United States)

^c LAWRENCE BERKELEY NATIONAL LABORATORY   (United States)

^d University of California at Berkeley   (United States)

Author keywords

CRISPR Cas9; Mevalonate; Multiplex genome editing; Off target analysis; Yeast

Indexed keywords

BIOCHEMISTRY; GENES; METABOLISM; MULTIPLEXING; YEAST;

CRISPR/CAS9; ENGINEERED STRAINS; EXPLORATORY ANALYSIS; FUNCTIONAL GENOMICS; ISOPRENOID BIOSYNTHESIS; METABOLIC PATHWAY ENGINEERING; MEVALONATE; TARGET ANALYSIS;

METABOLIC ENGINEERING;

FUNGAL RNA; ISOPRENOID; MEVALONIC ACID;

ARTICLE; BIOSYNTHESIS; CLONING; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; CONTROLLED STUDY; ENZYME ACTIVITY; FUNCTIONAL GENOMICS; FUNGAL GENOME; FUNGAL STRAIN; GENE DISRUPTION; GENETIC ENGINEERING; GENETIC VARIABILITY; METABOLIC ENGINEERING; NONHUMAN; PLASMID; PRIORITY JOURNAL; SACCHAROMYCES CEREVISIAE; CRISPR CAS SYSTEM; GENETICS; PROCEDURES;

SACCHAROMYCES CEREVISIAE;

CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEATS; CRISPR-CAS SYSTEMS; GENETIC ENGINEERING; GENOME, FUNGAL; SACCHAROMYCES CEREVISIAE;

EID: 84923021733     PISSN: 10967176     EISSN: 10967184     Source Type: Journal    
DOI: 10.1016/j.ymben.2015.01.008     Document Type: Article

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