A rapid, generally applicable method to engineer zinc fingers illustrated by targeting the HIV-1 promoter

Nature Biotechnology

Volumn 19, Issue 7, 2001, Pages 656-660

  Isalan, Mark ^a,b   Klug, Aaron ^a   Choo, Yen ^a,b  

^a MRC LABORATORY OF MOLECULAR BIOLOGY   (United Kingdom)

^b Gendaq Ltd   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

DNA SEQUENCES; GENETIC ENGINEERING; PROTEINS; VIRUSES;

HUMAN IMMUNODEFICIENCY VIRUS (HIV);

BIOTECHNOLOGY;

DNA; TRANSCRIPTION FACTOR; ZINC FINGER PROTEIN;

ARTICLE; BINDING AFFINITY; BIOENGINEERING; DISSOCIATION CONSTANT; DNA BINDING; GENETIC ENGINEERING; HUMAN IMMUNODEFICIENCY VIRUS 1; METHODOLOGY; MOLECULAR RECOGNITION; PHAGE DISPLAY; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN DOMAIN;

AMINO ACID SEQUENCE; BASE SEQUENCE; BINDING, COMPETITIVE; DNA; DNA-BINDING PROTEINS; GENETIC TECHNIQUES; HIV-1; KINETICS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; PEPTIDE LIBRARY; PEPTIDES; PROMOTER REGIONS (GENETICS); PROTEIN BINDING; PROTEIN STRUCTURE, TERTIARY; RECOMBINATION, GENETIC; SEQUENCE HOMOLOGY, AMINO ACID; SEQUENCE HOMOLOGY, NUCLEIC ACID; ZINC FINGERS;

HUMAN IMMUNODEFICIENCY VIRUS; HUMAN IMMUNODEFICIENCY VIRUS 1; RNA VIRUSES;

EID: 0034930363     PISSN: 10870156     EISSN: None     Source Type: Journal    
DOI: 10.1038/90264     Document Type: Article

References (32)

1. In vivo repression by a site-specific DNA-binding protein designed against an oncogenic sequence
2. A general strategy for selecting high-affinity zinc finger proteins for diverse DNA target sites
3. 'Zinc fingers': A novel protein motif for nucleic acid recognition
4. Designing DNA-binding proteins on the surface of filamentous phage
5. Repetitive zinc-binding domains in the protein transcription factor IIIA from Xenopus oocytes
6. Zinc finger-DNA recognition: Crystal structure of a Zif268-DNA complex at 2.1 Å
7. Zinc finger phage: Affinity selection of fingers with new DNA-binding specificities
8. In vitro selection of zinc fingers with altered DNA-binding specificity
9. Toward a code for the interactions of zinc fingers with DNA: Selection of randomised zinc fingers displayed on phage
10. Building zinc fingers by selection: Toward a therapeutic application
11. Comprehensive DNA recognition through concerted interactions from adjacent zinc fingers
12. Recognition of DNA methylation by zinc fingers
13. Toward controlling gene expression at will: Selection and design of zinc finger domains recognising each of the 5′-GNN-3′ DNA target sequences
14. Insights into the molecular recognition of the 5′-GNN-3′ family of DNA sequences by zinc finger domains
15. Engineered zinc finger proteins that recognise DNA modification by Haelll and Hhal methyltransferase enzymes
16. Positive and negative regulation of endogenous genes by designed transcription factors
17. Advances in zinc finger engineering
18. Analysis of zinc fingers optimised via phage display: Evaluating the utility of a recognition code
19. Synergy between adjacent zinc fingers in sequence-specific DNA recognition
20. A gene activated in mouse 3T3 cells by serum growth factors encodes a protein with "zinc finger" sequences
21. Selection of DNA binding sites for zinc fingers using rationally randomised DNA reveals coded interactions
22. From repression domains to designer zinc finger proteins: A novel strategy of cellular immunisation against HIV
23. Building zinc fingers by selection: Toward a therapeutic application
24. Design of polydactyl zinc-finger proteins for unique addressing within complex genomes
25. Getting a handhold on DNA: Design of poly-zinc finger proteins with femtomolar dissociation constants
26. Design of polyzinc finger peptides with structured linkers
27. Improved DNA-binding specificity from polyzinc finger peptides by using strings of two-finger units
28. Physical basis of a protein-DNA recognition code
29. Zinc finger proteins as designer transcription factors
30. Regulation of an endogenous locus using a panel of designed zinc finger proteins targeted to accessible chromatin regions. Activation of vascular endothelial growth factor A
31. Synthetic zinc finger transcription factor action at an endogenous chromosomal site. Activation of the human erythropoietin gene
32. Zif268 protein-DNA complex refined at 1.6A: A model system for understanding zinc finger interactions