Improving the secretory capacity of Chinese hamster ovary cells by ectopic expression of effector genes: Lessons learned and future directions

Biotechnology Advances

Volumn 35, Issue 1, 2017, Pages 64-76

  Hansen, Henning Gram ^a   Pristovšek, Nuša ^a   Kildegaard, Helene Faustrup ^a   Lee, Gyun Min ^a,b  

^a Chalmers University of Technology   (Denmark)

^b Korea Advanced Institute of Science and Technology (KAIST)   (South Korea)

Author keywords

Cell engineering; Chinese hamster ovary (CHO) cells; Ectopic expression; Endoplasmic reticulum; ER stress; Gene dosage; Product quality; Recombinant protein production; Secretion bottleneck; Specific productivity

Indexed keywords

CELL MEMBRANES; CELLS; CYTOLOGY; GENE EXPRESSION; GENES; PROTEINS; RECOMBINANT PROTEINS;

CHINESE HAMSTER OVARY CELLS; ECTOPIC EXPRESSIONS; ENDOPLASMIC RETICULUM; ER STRESS; GENE DOSAGE; RECOMBINANT PROTEIN PRODUCTIONS; SECRETION BOTTLENECK; SPECIFIC PRODUCTIVITY;

CELL ENGINEERING;

CELLS AND CELL COMPONENTS; EXPERIMENTAL STUDY; GENE EXPRESSION; GENETIC ANALYSIS; GENETIC ENGINEERING; OPTIMIZATION; PROTEIN; RODENT; SECRETION;

CRICETULUS GRISEUS;

RECOMBINANT PROTEIN;

ANIMAL; CELL ENGINEERING; CHO CELL LINE; CRICETULUS; ECTOPIC EXPRESSION; ENDOPLASMIC RETICULUM; GENE DOSAGE; GENETICS; HAMSTER; METABOLISM; PROCEDURES; SECRETION (PROCESS);

ANIMALS; CELL ENGINEERING; CHO CELLS; CRICETINAE; CRICETULUS; ECTOPIC GENE EXPRESSION; ENDOPLASMIC RETICULUM; GENE DOSAGE; RECOMBINANT PROTEINS;

EID: 85008214376     PISSN: 07349750     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.biotechadv.2016.11.008     Document Type: Review

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