Heterologous expression of the lipid transfer protein CERT increases therapeutic protein productivity of mammalian cells

Journal of Biotechnology

Volumn 141, Issue 1-2, 2009, Pages 84-90

  Florin, Lore ^a   Pegel, Antje ^a   Becker, Eric ^a,b   Hausser, Angelika ^b   Olayioye, Monilola A ^b   Kaufmann, Hitto ^a  

^a BOEHRINGER INGELHEIM PHARMA GMBH AND CO KG   (Germany)

^b UNIVERSITY OF STUTTGART   (Germany)

Author keywords

Cell culture; CERT; CHO; Fed batch; Golgi; Recombinant protein production; Secretion engineering

Indexed keywords

CERT; CHO; FED BATCH; GOLGI; RECOMBINANT PROTEIN PRODUCTION; SECRETION ENGINEERING;

AMIDES; ANIMAL CELL CULTURE; BATCH CELL CULTURE; CELL MEMBRANES; MAMMALS; MONOCLONAL ANTIBODIES; PROTEINS;

CELL CULTURE;

CERAMIDE TRANSFER PROTEIN; IMMUNOGLOBULIN G; LIPID TRANSFER PROTEIN; MONOCLONAL ANTIBODY; PROTEIN KINASE D; UNCLASSIFIED DRUG;

ANTIBODY PRODUCTION; ARTICLE; CELL CULTURE; CELL GROWTH; CELL LINE; CELL MEMBRANE; CHO CELL; GOLGI COMPLEX; HETEROLOGOUS EXPRESSION; IMMUNOGLOBULIN PRODUCTION; INOCULATION; LIPID TRANSPORT; MAMMAL CELL; NONHUMAN; PLASMID; PRIORITY JOURNAL; PROTEIN ENGINEERING; PROTEIN EXPRESSION; PROTEIN SECRETION; PROTEIN TRANSPORT; SUSPENSION CELL CULTURE; WILD TYPE;

ANIMALS; BLOTTING, WESTERN; CARRIER PROTEINS; CHO CELLS; CRICETINAE; CRICETULUS; ENZYME-LINKED IMMUNOSORBENT ASSAY; FLOW CYTOMETRY; HUMANS; RECOMBINANT PROTEINS; SERUM ALBUMIN;

MAMMALIA;

EID: 64549145396     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2009.02.014     Document Type: Article

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