Characterization of chimeric antibody producing CHO cells in the course of dihydrofolate reductase-mediated gene amplification and their stability in the absence of selective pressure

Biotechnology and Bioengineering

Volumn 58, Issue 1, 1998, Pages 73-84

  Kim, Sang Jick ^a   Kim, No Soo ^a   Ryu, Chun Jeih ^b   Hong, Hyo Jeong ^b   Lee, Gyun Min ^a  

^a Korea Advanced Institute of Science and Technology (KAIST)   (South Korea)

^b Korea Research Institute of Bioscience and Biotechnology (KRIBB)   (South Korea)

Author keywords

Chimeric antibody; CHO cells; Dihydrofolate reductase; Flow cytometry; Gene copy number; Stability

Indexed keywords

ANTIBODIES; ANTIGENS; CELL CULTURE; GENES; GENETIC ENGINEERING; GROWTH KINETICS;

CHIMERIC ANTIBODY; CHINESE HAMSTER OVARY; DIHYDROFOLATE REDUCTADASE; FLOW CYTOMETRY; GENE AMPLIFICATION; GENE COPY NUMBER; IMMUNOGLOBULIN; SOUTHERN BLOT ANALYSIS;

CLONING;

CHIMERIC ANTIBODY; DIHYDROFOLATE REDUCTASE;

ANIMAL CELL; ARTICLE; CHO CELL; CULTURE MEDIUM; DNA TRANSFECTION; EXPRESSION VECTOR; FLOW CYTOMETRY; GENE AMPLIFICATION; NONHUMAN; NORTHERN BLOTTING; SOUTHERN BLOTTING;

ANIMALS; ANTIMETABOLITES, ANTINEOPLASTIC; BLOTTING, NORTHERN; BLOTTING, SOUTHERN; CHO CELLS; CRICETINAE; FLOW CYTOMETRY; GENE AMPLIFICATION; HEPATITIS B ANTIBODIES; HEPATITIS B SURFACE ANTIGENS; METHOTREXATE; RECOMBINANT FUSION PROTEINS; TETRAHYDROFOLATE DEHYDROGENASE;

ANIMALIA; CRICETINAE; CRICETULUS GRISEUS; HEPATITIS B VIRUS;

EID: 0002598959     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0290(19980405)58:1<73::AID-BIT8>3.0.CO;2-R     Document Type: Article

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