Functional heterogeneity and heritability in CHO cell populations

Biotechnology and Bioengineering

Volumn 110, Issue 1, 2013, Pages 260-274

  Davies, Sarah L ^a   Lovelady, Clare S ^a   Grainger, Rhian K ^a   Racher, Andrew J ^b   Young, Robert J ^c   James, David C ^a  

^a UNIVERSITY OF SHEFFIELD   (United Kingdom)

^b LONZA AG   (Switzerland)

^c Lonza Biologics plc ^*  (United Kingdom)

Author keywords

Chinese hamster ovary cells; Directed evolution; Genetic heterogeneity; Recombinant monoclonal antibody; Screening

Indexed keywords

CELL CULTURE; CELL MEMBRANES; CELL PROLIFERATION; CLONING; MONOCLONAL ANTIBODIES; SCREENING;

ANALYSIS OF VARIATIONS; CELL BIOMASS; CELL SURFACES; CELLULAR PROTEINS; CHINESE HAMSTER OVARY CELLS; CHO CELL; CLONAL POPULATION; CRYO-PRESERVATION; DIRECTED EVOLUTION; DYNAMIC NATURE; FUNCTIONAL CAPABILITIES; FUNCTIONAL GENOMES; FUNCTIONAL HETEROGENEITY; FUNCTIONAL PROPERTIES; GENETIC DRIFTS; GENETIC HETEROGENEITIES; GREEN FLUORESCENT PROTEIN; IN-VITRO; MICROPLATES; N-GLYCAN PROCESSING; N-GLYCOSYLATION; PARENTAL CELLS; PLASMID VECTORS; PRODUCTION CAPABILITIES; PROLIFERATION RATE; PROTEIN CONTENTS; RECOMBINANT MONOCLONAL ANTIBODY; SUSPENSION CULTURES;

CLONE CELLS;

GLYCAN; GREEN FLUORESCENT PROTEIN; MONOCLONAL ANTIBODY; PLASMID VECTOR;

ANIMAL CELL; ARTICLE; BIOMASS; CELL POPULATION; CELL PROLIFERATION; CELL SIZE; CELL SURFACE; CHO CELL; CONTROLLED STUDY; CRYOPRESERVATION; DILUTION; FEMALE; GENETIC HETEROGENEITY; GENETIC TRANSFECTION; GENETIC VARIABILITY; GLYCOSYLATION; HERITABILITY; IMAGE ANALYSIS; IN VITRO STUDY; MOLECULAR CLONING; NONHUMAN; PROTEIN CONTENT;

CRICETULUS GRISEUS;

EID: 84869878604     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.24621     Document Type: Article

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