Synthetic promoters for CHO cell engineering

Biotechnology and Bioengineering

Volumn 111, Issue 8, 2014, Pages 1638-1647

  Brown, Adam J ^a   Sweeney, Bernie ^b   Mainwaring, David O ^b   James, David C ^a  

^a UNIVERSITY OF SHEFFIELD   (United Kingdom)

^b UCB   (United Kingdom)

Author keywords

Cell engineering; Chinese hamster ovary cells; Synthetic biology; Synthetic promoter

Indexed keywords

BINDING SITES; BIOINFORMATICS; CELL CULTURE; CELL ENGINEERING; GENES; PHOSPHATASES; TRANSCRIPTION FACTORS;

CHINESE HAMSTER OVARY CELLS; HUMAN CYTOMEGALOVIRUS; SECRETED ALKALINE PHOSPHATASE; SYNTHETIC BIOLOGY; SYNTHETIC PROMOTER; TRANSCRIPTION FACTOR BINDING SITES; TRANSCRIPTIONAL ACTIVITY; TRANSIENT EXPRESSION;

CELLS;

ALKALINE PHOSPHATASE; CCAAT ENHANCER BINDING PROTEIN ALPHA; CCAAT ENHANCER BINDING PROTEIN ALPHA 1; IMMUNOGLOBULIN ENHANCER BINDING PROTEIN; NUCLEAR FACTOR KAPPA B 5; TRANSCRIPTION FACTOR; TRANSCRIPTION FACTOR E BOX 3; TRANSCRIPTION FACTOR GC BOX 1; TRANSCRIPTION FACTOR REGULATORY ELEMENT; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; BINDING SITE; BIOINFORMATICS; CELL ENGINEERING; CHO CELL LINE; CONTROLLED STUDY; CYTOMEGALOVIRUS; FEMALE; GENE EXPRESSION REGULATION; GENE INSERTION; GENE SEQUENCE; NONHUMAN; NUCLEOTIDE SEQUENCE; PROMOTER REGION; RECOMBINANT GENE; REPORTER GENE; SEQUENCE ANALYSIS; SYNTHESIS; ANIMAL; CRICETULUS; GENETIC ENGINEERING; GENETIC TRANSFECTION; GENETICS; HUMAN; METABOLISM; MOLECULAR GENETICS; PROCEDURES; TRANSCRIPTION INITIATION;

ANIMALS; BASE SEQUENCE; CELL ENGINEERING; CHO CELLS; CRICETULUS; GENES, REPORTER; GENETIC ENGINEERING; HUMANS; MOLECULAR SEQUENCE DATA; PROMOTER REGIONS, GENETIC; TRANSCRIPTION FACTORS; TRANSCRIPTIONAL ACTIVATION; TRANSFECTION;

EID: 84903180753     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.25227     Document Type: Article

References (39)

1. Al-Fageeh MB, Marchant RJ, Carden MJ, Smales CM. 2006. The cold-shock response in cultured mammalian cells: Harnessing the response for the improvement of recombinant protein production. Biotechnol Bioeng 93(5):829-835.
2. Blazeck J, Garg R, Reed B, Alper HS. 2012. Controlling promoter strength and regulation in Saccharomyces cerevisiae using synthetic hybrid promoters. Biotechnol Bioeng 109(11):2884-2895.
3. Brown AJ, Mainwaring DO, Sweeney B, James DC. 2013. Block decoys: Transcription factor decoys designed for in vitro gene regulation studies. Anal Biochem 443(2):205-210.
4. Dale L. 2006. Mammalian expression cassette engineering for high-level protein production. BioProcess Int 4:14-22.
5. Daramola O, Stevenson J, Dean G, Hatton D, Pettman G, Holmes W, Field R. 2013. A high-yielding CHO trasnient system: Coexpression of genes encoding EBNA-1 and GS enhances transient protein expression. Biotechnol Prog 30:132-141.
6. Datta P, Linhardt RJ, Sharfstein ST. 2013. An'omics approach towards CHO cell engineering. Biotechnol Bioeng 110(5):1255-1271.
7. Dolcet X, Llobet D, Pallares J, Matias-Guiu X. 2005. NF-kB in development and progression of human cancer. Virchows Arch 446(5):475-482.
8. Ferreira JP, Overton KW, Wang CL. 2013. Tuning gene expression with synthetic upstream open reading frames. Proc Natl Acad Sci USA 110(28):11284-11289.
9. Ferreira JP, Peacock RW, Lawhorn IE, Wang CL. 2011. Modulating ectopic gene expression levels by using retroviral vectors equipped with synthetic promoters. Syst Biol Synth Biol 5(3-4):131-138.
10. Girod PA, Zahn-Zabal M, Mermod N. 2005. Use of the chicken lysozyme 5′ matrix attachment region to generate high producer CHO cell lines. Biotechnol Bioeng 91(1):1-11.
11. Grabherr MG, Pontiller J, Mauceli E, Ernst W, Baumann M, Biagi T, Swofford R, Russell P, Zody MC, Di Palma F. 2011. Exploiting nucleotide composition to engineer promoters. PLoS ONE 6(5):e20136.
12. Hai T, Curran T. 1991. Cross-family dimerization of transcription factors Fos/Jun and ATF/CREB alters DNA binding specificity. Proc Natl Acad Sci USA 88(9):3720-3724.
13. Ho SC, Koh EY, van Beers M, Mueller M, Wan C, Teo G, Song Z, Tong Y, Bardor M, Yang Y. 2013. Control of IgG LC: HC ratio in stably transfected CHO cells and study of the impact on expression, aggregation, glycosylation and conformational stability. J Biotechnol 165(3-4):157-166.
14. Juven-Gershon T, Cheng S, Kadonaga J. 2006. Rational design of a super core promoter that enhances gene expression. Nat Methods 3(11):917-922.
15. Juven-Gershon T, Kadonaga JT. 2010. Regulation of gene expression via the core promoter and the basal transcriptional machinery. Dev Biol 339(2):225-229.
16. Kim M, O'Callaghan PM, Droms KA, James DC. 2011. A mechanistic understanding of production instability in CHO cell lines expressing recombinant monoclonal antibodies. Biotechnol Bioeng 108(10):2434-2446.
17. Le H, Vishwanathan N, Kantardjieff A, Doo I, Srienc M, Zheng X, Somia N, Hu W-S. 2013. Dynamic gene expression for metabolic engineering of mammalian cells in culture. Metab Eng 20:212-220.
18. Lewis NE, Liu X, Li Y, Nagarajan H, Yerganian G, O'Brien E, Bordbar A, Roth AM, Rosenbloom J, Bian C. 2013. Genomic landscapes of Chinese hamster ovary cell lines as revealed by the Cricetulus griseus draft genome. Nat Biotechnol 31(8):759-765.
19. Lin CY, Lovén J, Rahl PB, Paranal RM, Burge CB, Bradner JE, Lee TI, Young RA. 2012. Transcriptional amplification in tumor cells with elevated c-Myc. Cell 151(1):56-67.
20. Mader A, Prewein B, Zboray K, Casanova E, Kunert R. 2012. Exploration of BAC versus plasmid expression vectors in recombinant CHO cells. Appl Microbiol Biotechnol 97(9):4049-4054.
21. Manke T, Roider HG, Vingron M. 2008. Statistical modeling of transcription factor binding affinities predicts regulatory interactions. PLoS Comput Biol 4(3):e1000039.
22. McLeod J, O'Callaghan PM, Pybus LP, Wilkinson SJ, Root T, Racher AJ, James DC. 2011. An empirical modeling platform to evaluate the relative control discrete CHO cell synthetic processes exert over recombinant monoclonal antibody production process titer. Biotechnol Bioeng 108(9):2193-2204.
23. O'Callaghan PM, McLeod J, Pybus LP, Lovelady CS, Wilkinson SJ, Racher AJ, Porter A, James DC. 2010. Cell line-specific control of recombinant monoclonal antibody production by CHO cells. Biotechnol Bioeng 106(6):938-951.
24. Ogawa R, Kagiya G, Kodaki T, Fukuda S, Yamamoto K. 2007. Construction of strong mammalian promoters by random cis-acting element elongation. BioTechniques 42(5):628.
25. Pasotti L, Politi N, Zucca S, De Angelis MGC, Magni P. 2012. Bottom-up engineering of biological systems through standard bricks: A modularity study on basic parts and devices. PLoS ONE 7(7):e39407.
26. Prentice H, Tonkin C, Caamano L, Sisk W. 2007. High level expression of proteins using sequences from the ferritin heavy chain gene locus. J Biotechnol 128(1):50-60.
27. Pybus LP, Dean G, West NR, Smith A, Daramola O, Field R, Wilkinson SJ, James DC. 2013. Model-directed engineering of "difficult-to-express" monoclonal antibody production by Chinese hamster ovary cells. Biotechnol Bioeng 111:372-385.
28. Schlabach MR, Hu JK, Li M, Elledge SJ. 2010. Synthetic design of strong promoters. Proc Natl Acad Sci USA 107(6):2538.
29. Schug J. 2008. Using TESS to predict transcription factor binding sites in DNA sequence. Curr Protoc Bioinform 21:2.6.1-2.6.15.
30. Seghezzi N, Amar P, Koebmann B, Jensen PR, Virolle M-J. 2011. The construction of a library of synthetic promoters revealed some specific features of strong Streptomyces promoters. Appl Microbiol Biotechnol 90(2):615-623.
31. Sleight SC, Bartley BA, Lieviant JA, Sauro HM. 2010. Designing and engineering evolutionary robist genetic circuits. J Biol Eng 4:12.
32. Stadlmayr G, Mecklenbräuker A, Rothmüller M, Maurer M, Sauer M, Mattanovich D, Gasser B. 2010. Identification and characterisation of novel Pichia pastoris promoters for heterologous protein production. J Biotechnol 150(4):519-529.
33. Stinski MF, Isomura H. 2008. Role of the cytomegalovirus major immediate early enhancer in acute infection and reactivation from latency. Med Microbiol Immunol 197(2):223-231.
34. Tigges M, Fussenegger M. 2009. Recent advances in mammalian synthetic biology-design of synthetic transgene control networks. Curr Opin Biotechnol 20(4):449-460.
35. Tornøe J, Kusk P, Johansen TE, Jensen PR. 2002. Generation of a synthetic mammalian promoter library by modification of sequences spacing transcription factor binding sites. Gene 297(1):21-32.
36. Weber W, Fussenegger M. 2010. Synthetic gene networks in mammalian cells. Curr Opin Biotechnol 21(5):690-696.
37. Xu X, Nagarajan H, Lewis NE, Pan S, Cai Z, Liu X, Chen W, Xie M, Wang W, Hammond S. 2011. The genomic sequence of the Chinese hamster ovary (CHO)-K1 cell line. Nat Biotechnol 29(8):735-741.
38. Yim SS, An SJ, Kang M, Lee J, Jeong KJ. 2013. Isolation of fully synthetic promoters for high-level gene expression in Corynebacterium glutamicum. Biotechnol Bioeng 110(11):2959-2969.
39. Zhou H, Liu Z-g, Sun Z-w, Huang Y, Yu W-y. 2010. Generation of stable cell lines by site-specific integration of transgenes into engineered Chinese hamster ovary strains using an FLP-FRT system. J Biotechnol 147(2):122-129.