Uncovering methods for the prevention of protein aggregation and improvement of product quality in a transient expression system

Biotechnology Progress

Volumn 31, Issue 1, 2015, Pages 258-267

  Estes, Bram ^a   Hsu, Yueh Rong ^a   Tam, Lei Ting ^a   Sheng, Jackie ^a   Stevens, Jennitte ^a   Haldankar, Raj ^b  

^a AMGEN INC   (United States)

^b NGM Biopharmaceuticals Inc   (United States)

Author keywords

293 EBNA1; Aggregation; Gene dosage; Mild hypothermia; Transient expression

Indexed keywords

AGGLOMERATION; MAMMALS; MOLECULES; QUALITY CONTROL; TRANSCRIPTION;

293-EBNA1; GENE DOSAGE; HYPOTHERMIC CONDITIONS; IMPROVEMENT IN PRODUCT QUALITY; N-LINKED GLYCOSYLATION; SIGNAL PEPTIDE CLEAVAGES; THERAPEUTIC MOLECULES; TRANSIENT EXPRESSION;

RECOMBINANT PROTEINS;

MAMMALIA;

HYBRID PROTEIN; PROTEIN AGGREGATE;

CHEMISTRY; CULTURE TECHNIQUE; GENE DOSAGE; GENETIC TRANSFECTION; GENETICS; GLYCOSYLATION; HEK293 CELL LINE; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; PROCEDURES; TEMPERATURE;

CELL CULTURE TECHNIQUES; GENE DOSAGE; GLYCOSYLATION; HEK293 CELLS; HUMANS; PROTEIN AGGREGATES; RECOMBINANT FUSION PROTEINS; TEMPERATURE; TRANSFECTION;

EID: 84923520159     PISSN: 87567938     EISSN: 15206033     Source Type: Journal    
DOI: 10.1002/btpr.2021     Document Type: Article

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