Recombinant protein production by large-scale transient gene expression in mammalian cells: State of the art and future perspectives

Biotechnology Letters

Volumn 29, Issue 5, 2007, Pages 677-684

  Baldi, Lucia ^a   Hacker, David L ^a   Adam, Myriam ^a   Wurm, Florian M ^a  

^a EPFL   (Switzerland)

Author keywords

Mammalian cells; Non viral gene delivery; Recombinant protein; Transient gene expression

Indexed keywords

ANIMALS; CELLS; DRUG PRODUCTS; DRUG THERAPY; GENETIC ENGINEERING; RESEARCH AND DEVELOPMENT MANAGEMENT;

MAMMALIAN CELLS; NON-VIRAL GENE DELIVERY; RECOMBINANT PROTEIN; TRANSIENT GENE EXPRESSION;

PROTEINS;

RECOMBINANT PROTEIN;

ANIMAL; BIOSYNTHESIS; BIOTECHNOLOGY; GENE EXPRESSION; GENE TRANSFER; GENE VECTOR; HUMAN; METHODOLOGY; REVIEW;

ANIMALS; BIOTECHNOLOGY; GENE EXPRESSION; GENE TRANSFER TECHNIQUES; GENETIC VECTORS; HUMANS; RECOMBINANT PROTEINS;

MAMMALIA;

EID: 34147125994     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-006-9297-y     Document Type: Review

References (59)

1. Baldi L, Muller N, Picasso S et al (2005) Transient gene expression in suspension HEK-293 cells: application to large-scale protein production. Biotechnol Prog 21(1):148-153
2. Barnes D, Sato G (1980) Serum-free cell culture: a unifying approach. Cell 22(3):649-655
3. Batard P, Jordan M, Wurm F (2001) Transfer of high copy number plasmid into mammalian cells by calcium phosphate transfection. Gene 270(1-2):61-68
4. Bentley KJ, Gewert R, Harris WJ (1998) Differential efficiency of expression of humanized antibodies in transient transfected mammalian cells. Hybridoma 17(6):559-567
5. Blasey HD, Brethon B, Hovius R et al (2000) Large scale transient 5-HT3 receptor production with the Semliki Forest Virus Expression System. Cytotechnology 32(3):199-208
6. Boussif O, Lezoualc'h F, Zanta MA et al (1995) A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: polyethylenimine. Proc Natl Acad Sci USA 92(16):7297-7301
7. Boyce FM, Bucher NL (1996) Baculovirus-mediated gene transfer into mammalian cells. Proc Natl Acad Sci USA 93(6):2348-2352
8. Buchman AR, Berg P (1988) Comparison of intron-dependent and intron-independent gene expression. Mol Cell Biol 8(10):4395-4405
9. Cho MS, Yee H et al (2002) Establishment of a human somatic hybrid cell line for recombinant protein production. J Biomed Sci 9(6 Pt 2):631-638
10. Cho MS, Yee H, Brown C et al (2003) Versatile expression system for rapid and stable production of recombinant proteins. Biotechnol Prog 19(1):229-232
11. Côté J, Garnier A, Massie B et al (1998) Serum-free production of recombinant proteins and adenoviral vectors by 293SF-3F6 cells. Biotechnol Bioeng 59(5):567-575
12. Dang JM, Leong KW (2006) Natural polymers for gene delivery and tissue engineering. Adv Drug Deliv Rev 58(4):487-499
13. Davies A, Greene A, Lullau E et al (2005) Optimisation and evaluation of a high-throughput mammalian protein expression system. Protein Expr Purif 42(1):111-121
14. Derouazi M, Girard P, Van Tilborgh F et al (2004) Serum-free large-scale transient transfection of CHO cells. Biotechnol Bioeng 87(4):537-545
15. Durocher Y, Perret S, Kamen A (2002) High-level and high-throughput recombinant protein production by transient transfection of suspension-growing human 293-EBNA1 cells. Nucleic Acids Res 30(2):E9
16. Edwards RH, Selby MJ, Garcia PD et al (1988) Processing of the native nerve growth factor precursor to form biologically active nerve growth factor. J Biol Chem 263(14):6810-6815
17. Falkner FG, Turecek PL, MacGillivray RT et al (1992) High level expression of active human prothrombin in a vaccinia virus expression system. Thromb Haemost 68(2):119-124
18. Fussenegger M, Bailey JE (1998) Molecular regulation of cell-cycle progression and apoptosis in mammalian cells: implications for biotechnology. Biotechnol Prog 14(6):807-833
19. Galbraith DJ, Tait AS, Racher AJ et al (2006) Control of culture environment for improved polyethylenimine-mediated transient production of recombinant monoclonal antibodies by CHO cells. Biotechnol Prog 22(3):753-762
20. Garnier A, Côté J, Nadeau I et al (1994) Scale-up of the adenovirus expression system for the production of recombinant protein in human 293S cells. Cytotechnology 15(1-3):145-155
21. Geisse S, Henke M (2005) Large-scale transient transfection of mammalian cells: a newly emerging attractive option for recombinant protein production. J Struct Funct Genomics 6(2-3):165-170
22. Gill DR, Smyth SE, Goddard CA et al (2001) Increased persistence of lung gene expression using plasmids containing the ubiquitin C or elongation factor 1alpha promoter. Gene Ther 8(20):1539-1546
23. Girard P, Derouazi M, Baumgartner G et al (2002) 100-liter transient transfection. Cytotechnology 38:15-21
24. Graham FL, Smiley J, Russell WC et al (1977) Characteristics of a human cell line transformed by DNA from human adenovirus type 5. J Gen Virol 36(1):59-74
25. Graham FL (1987) Growth of 293 cells in suspension culture. J Gen Virol 68(Pt 3):937-940
26. Hacker DL, Bertschinger M, Baldi L et al (2004) Reduction of adenovirus E1A mRNA by RNAi results in enhanced recombinant protein expression in transiently transfected HEK293 cells. Gene 341:227-234
27. Hassaine G, Wagner R, Kempf J et al (2006) Semliki Forest virus vectors for overexpression of 101 G protein-coupled receptors in mammalian host cells. Protein Expr Purif 45(2):343-351
28. Hofmann C, Sandig V, Jennings G et al (1995) Efficient gene transfer into human hepatocytes by baculovirus vectors. Proc Natl Acad Sci USA 92(22):10099-10103
29. Jarvis DL (2003) Developing baculovirus-insect cell expression systems for humanized recombinant glycoprotein production. Virology 310(1):1-7
30. Jordan M, Schallhorn A, Wurm FM (1996) Transfecting mammalian cells: optimization of critical parameters affecting calcium-phosphate precipitate formation. Nucleic Acids Res 24(4):596-601
31. Kim NS, Lee GM (2002) Response of recombinant Chinese hamster ovary cells to hyperosmotic pressure: effect of Bcl-2 overexpression. J Biotechnol 95(3):237-248
32. Kost TA, Klein JL, Condreay JP (2000) Application of Recombinant Baculoviruses in Biopharmaceutical Research. In: Al-Rubeai M, Fussenegger M (eds) Cell engineering, Kluwer Academic Publishers
33. Lechardeur D, Verkman AS, Lukacs GL (2005) Intracellular routing of plasmid DNA during non-viral gene transfer. Adv Drug Deliv Rev 57(5):755-767
34. Lee CC, MacKay JA, Frechet JM et al (2005) Designing dendrimers for biological applications. Nat Biotechnol 23(12):1517-1526
35. Li LH, Shivakumar R, Feller S et al (2002) Highly efficient, large volume flow electroporation. Technol Cancer Res Treat 1(5):341-350
36. Lindell J, Girard P, Muller N et al (2004) Calfection: a novel gene transfer method for suspension cells. Biochim Biophys Acta 1676(2):155-161
37. Makrides SC (1999) Components of vectors for gene transfer and expression in mammalian cells. Protein Expr Purif 17(2):183-202
38. Mastrangelo AJ, Hardwick JM, Zou S et al (2000) Part II. Overexpression of bcl-2 family members enhances survival of mammalian cells in response to various culture insults. Biotechnol Bioeng 67(5):555-564
39. Meissner P, Pick H, Kulangara A et al (2001) Transient gene expression: recombinant protein production with suspension-adapted HEK293-EBNA cells. Biotechnol Bioeng 75(2):197-203
40. Muller N (2005) Transient gene expression for rapid protein production: studies & optimizations under serum-free conditions. Dissertation, Ècole Polytechnique Fédérale de Lausanne
41. Pavirani A, Meulien P, Harrer H et al (1987) Two independent domains of factor VIII co-expressed using recombinant vaccinia viruses have procoagulant activity. Biochem Biophys Res Commun 145(1):234-240
42. Pham PL, Perret S, Doan HC et al (2003) Large-scale transient transfection of serum-free suspension-growing HEK293 EBNA1 cells: peptone additives improve cell growth and transfection efficiency. Biotechnol Bioeng 84(3):332-342
43. Pham PL, Perret S, Cass B et al (2005) Transient gene expression in HEK293 cells: peptone addition posttransfection improves recombinant protein synthesis. Biotechnol Bioeng 90(3):332-344
44. Rosser MP, Xia W, Hartsell S et al (2005) Transient transfection of CHO-K1-S using serum-free medium in suspension: a rapid mammalian protein expression system. Protein Expr Purif 40(2):237-243
45. Schlaeger EJ, Christensen K (1999) Transient gene expression in mammalian cells grown in serum-free suspension culture. Cytotechnology 30:71-83
46. Seo NS, Hollister JR, Jarvis DL (2001) Mammalian glycosyltransferase expression allows sialoglycoprotein production by baculovirus-infected insect cells. Protein Expr Purif 22(2):234-241
47. Shi C, Shin YO, Hanson J et al (2005) Purification and characterization of a recombinant G-protein-coupled receptor, Saccharomyces cerevisiae Ste2p, transiently expressed in HEK293 EBNA1 cells. Biochemistry 44(48):15705-15714
48. Stadler J, Lemmens R, Nyhammar T (2004) Plasmid DNA purification. J Gene Med 6 Supply 1:S54-S66
49. Sutter G, Moss B (1992) Nonreplicating vaccinia vector efficiently expresses recombinant genes. Proc Natl Acad Sci USA 89(22):10847-10851
50. Tait AS, Brown CJ, Galbraith DJ et al (2004) Transient production of recombinant proteins by Chinese hamster ovary cells using polyethyleneimine/DNA complexes in combination with microtubule disrupting anti-mitotic agents. Biotechnol Bioeng 88(6):707-721
51. Tey BT, Singh RP, Piredda L et al (2000) Influence of bcl-2 on cell death during the cultivation of a Chinese hamster ovary cell line expressing a chimeric antibody. Biotechnol Bioeng 68(1):31-43
52. Thomas M, Klibanov AM (2003) Non-viral gene therapy: polycation-mediated DNA delivery. Appl Microbiol Biotechnol 62(1):27-34
53. Thomas M, Lu JJ, Ge Q et al (2005) Full deacylation of polyethylenimine dramatically boosts its gene delivery efficiency and specificity to mouse lung. Proc Natl Acad Sci USA 102(16):5679-5684
54. Urlaub G, Chasin LA (1980) Isolation of Chinese hamster cell mutants deficient in dihydrofolate reductase activity. Proc Natl Acad Sci USA 77(7):4216-4220
55. Van Craenenbroeck K, Vanhoenacker P, Haegeman G (2000) Episomal vectors for gene expression in mammalian cells. Eur J Biochem 267(18):5665-5678
56. Wakelin SJ, Sabroe I, Gregory CD et al (2006) "Dirty little secrets"-Endotoxin contamination of recombinant proteins. Immunol Lett 106(1):1-7
57. Wright JL, Jordan M, Wurm FM (2003) Transfection of partially purified plasmid DNA for high level transient protein expression in HEK293-EBNA cells. J Biotechnol 102(3):211-221
58. Wurm FM (2004) Production of recombinant protein therapeutics in cultivated mammalian cells. Nat Biotechnol 22(11):1393-1398
59. Young JM, Cheadle C, Foulke JJS et al (1988) Utilization of an Epstein-Barr virus replicon as a eukaryotic expression vector. Gene 62(2):171-185