High-level recombinant protein production in CHO cells using lentiviral vectors and the cumate gene-switch

Biotechnology and Bioengineering

Volumn 106, Issue 2, 2010, Pages 203-215

  Gaillet, Bruno ^a,b   Gilbert, Rénald ^a,c   Broussau, Sophie ^a   Pilotte, Amélie ^a   Malenfant, Félix ^a   Mullick, Alaka ^a   Garnier, Alain ^b   Massie, Bernard ^a,b,d  

^a NATIONAL RESEARCH COUNCIL CANADA   (Canada)

^b UNIVERSITÉ LAVAL   (Canada)

^c MCGILL UNIVERSITY   (Canada)

^d UNIVERSITÉ DE MONTRÉAL   (Canada)

Author keywords

CHO cells; Cumate gene switch; Lentivirus; Recombinant protein production; Reduced temperature; Stable expression

Indexed keywords

CHO CELL; CHO CELLS; CUMATE GENE-SWITCH; RECOMBINANT PROTEIN PRODUCTIONS; TEMPERATURE STABLE;

ANTIBODIES; CELLS; LAKES; PHOSPHATASES; TRANSCRIPTION;

CELL CULTURE;

ALKALINE PHOSPHATASE; CD200 ANTIGEN; CHIMERIC ANTIBODY; COMPLEMENTARY DNA; ERYTHROPOIETIN; GREEN FLUORESCENT PROTEIN; IMMUNOGLOBULIN FC FRAGMENT; LENTIVIRUS VECTOR; RECOMBINANT PROTEIN; TRANSACTIVATOR PROTEIN;

ANIMAL CELL; ARTICLE; CELL CULTURE; GENE EXPRESSION; GENE SWITCHING; GENETIC TRANSCRIPTION; GENETIC TRANSDUCTION; INCUBATION TEMPERATURE; INCUBATION TIME; INFECTION; INTERNAL RIBOSOME ENTRY SITE; NONHUMAN; OVARY CELL; PROMOTER REGION; PROTEIN EXPRESSION; VIRAL GENE DELIVERY SYSTEM;

ANIMALS; BENZOATES; CHO CELLS; CRICETINAE; CRICETULUS; GENETIC ENHANCEMENT; GENETIC VECTORS; LENTIVIRUS; PROTEIN ENGINEERING; RECOMBINANT PROTEINS;

LENTIVIRUS;

EID: 77953069759     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.22698     Document Type: Article

References (56)

1. Andersen DC, Krummen L. 2002. Recombinant protein expression for therapeutic applications. Curr Opin Biotechnol 13:117-123. (Pubitemid 34310369)
2. Backliwal G, Hildinger M, Hasija V, Wurm FM. 2008. High-density transfection with HEK-293 cells allows doubling of transient titers and removes need for a priori DNA complex formation with PEI. Biotechnol Bioeng 99:721-727. (Pubitemid 351158270)
3. Bertschinger M, Schertenleib A, Cevey J, Hacker DL, Wurm FM. 2008. The kinetics of polyethylenimine-mediated transfection in suspension cultures of Chinese hamster ovary cells. Mol Biotechnol 40:136-143.
4. Bieniasz PD, Cullen BR. 2000. Multiple blocks to human immunodeficiency virus type 1 replication in rodent cells. J Virol 74:9868-9877.
5. Braun P, LaBaer J. 2003. High throughput protein production for functional proteomics. Trends Biotechnol 21:383-388. (Pubitemid 37025775)
6. Breunig M, Lungwitz U, Liebl R, Fontanari C, Klar J, Kurtz A, Blunk T, Goepferich A. 2005. Gene delivery with low molecular weight linear polyethylenimines. J Gene Med 7:1287-1298. (Pubitemid 41555435)
7. Broussau S, Jabbour N, Lachapelle G, Durocher Y, Tom R, Transfiguracion J, Gilbert R, Massie B. 2008. Inducible packaging cells for large-scale production of lentiviral vectors in serum-free suspension culture. Mol Ther 16:500-507. (Pubitemid 351314989)
8. Browne SM, Al-Rubeai M. 2007. Selection methods for high-producing mammalian cell lines. Trends Biotechnol 25:425-432. (Pubitemid 350147111)
9. Burns JC, Friedmann T, Driever W, Burrascano M, Yee JK. 1993. Vesicular stomatitis virus G glycoprotein pseudotyped retroviral vectors: Concentration to very high titer and efficient gene transfer into mammalian and nonmammalian cells. Proc Natl Acad Sci USA 90:8033-8037. (Pubitemid 23260849)
10. Caron AW, Nicolas C, Gaillet B, Ba I, Pinard M, Garnier A, Massie B, Gilbert R. 2009. Fluorescent labeling in semi-solid medium for selection of mammalian cells secreting high-levels of recombinant proteins. BMC Biotechnol 9:42.
11. Cass B, Pham PL, Kamen A, Durocher Y. 2005. Purification of recombinant proteins from mammalian cell culture using a generic double-affinity chromatography scheme. Protein Expr Purif 40:77-85. (Pubitemid 40240973)
12. Chang GD, Chen CJ, Lin CY, Chen HC, Chen H. 2003. Improvement of glycosylation in insect cells with mammalian glycosyltransferases. J Biotechnol 102:61-71. (Pubitemid 36369204)
13. Chuppa S, Tsai YS, Yoon S, Shackleford S, Rozales C, Bhat R, Tsay G, Matanguihan C, Konstantinov K, Naveh D. 1997. Fermentor temperature as a tool for control of high-density perfusion cultures of mammalian cells. Biotechnol Bioeng 55:328-338. (Pubitemid 27291201)
14. Derouazi M, Girard P, Van TF, Iglesias K, Muller N, Bertschinger M, Wurm FM. 2004. Serum-free large-scale transient transfection of CHO cells. Biotechnol Bioeng 87:537-545. (Pubitemid 39150055)
15. Durocher Y, Perret S, Kamen A. 2002. High-level and high-throughput recombinant protein production by transient transfection of suspension-growing human 293-EBNA1 cells. Nucleic Acids Res 30:E9.
16. Follenzi A, Sabatino G, Lombardo A, Boccaccio C, Naldini L. 2002. Efficient gene delivery and targeted expression to hepatocytes in vivo by improved lentiviral vectors. Hum Gene Ther 13:243-260. (Pubitemid 34164109)
17. Gaillet B, Gilbert R, Amziani R, Guilbault C, Gadoury C, Caron AW, Mullick A, Garnier A, Massie B. 2007. High-level recombinant protein production in CHO cells using an adenoviral vector and the cumate gene-switch. Biotechnol Prog 23:200-209. (Pubitemid 46293505)
18. Galbraith DJ, Tait AS, Racher AJ, Birch JR, James DC. 2006. Control of culture environment for improved polyethylenimine-mediated transient production of recombinant monoclonal antibodies by CHO cells. Biotechnol Prog 22:753-762.
19. Gervais C, Paquette D, Burns-Tardis A, Martin L, Massie B. 1998. Development of high output expression vectors for antibody production in mammalian cells. In: Nagai K, Wachi M, editors. Animal cell technology: Basic & applied aspects. Netherlands: Kluwer Academic Publishers. p 349-354.
20. Gilbert R, Broussau S, Massie B. 2007. Protein production using lentiviral vectors. In: Dyson MR, Durocher Y, editors. Methods express: Expression systems. Oxfordshire: Scion Publishing Ltd. p 241-258.
21. Gorczynski RM, Cattral MS, Chen Z, Hu J, Lei J, Min WP, Yu G, Ni J. 1999. An immunoadhesin incorporating the molecule OX-2 is a potent immunosuppressant that prolongs allo- and xenograft survival. J Immunol 163:1654-1660. (Pubitemid 29352791)
22. Havlis J, Thomas H, Sebela M, Shevchenko A. 2003. Fast-response proteomics by accelerated in-gel digestion of proteins. Anal Chem 75:1300-1306. (Pubitemid 36397161)
23. Jelkmann W. 2004. Molecular biology of erythropoietin. Intern Med 43:649-659.
24. Jorjani P, Ozturk SS. 1999. Effects of cell density and temperature on oxygen consumption rate for different mammalian cell lines. Biotechnol Bioeng 64:349-356. (Pubitemid 29297388)
25. Kafri T, van PH, Ouyang L, Gage FH, Verma IM. 1999. A packaging cell line for lentivirus vectors. J Virol 73:576-584. (Pubitemid 28565401)
26. Keller A, Nesvizhskii AI, Kolker E, Aebersold R. 2002. Empirical statistical model to estimate the accuracy of peptide identifications made by MS/MS and database search. Anal Chem 74:5383-5392. (Pubitemid 35215372)
27. Kordower JH, Emborg ME, Bloch J, Ma SY, Chu Y, Leventhal L, McBride J, Chen EY, Palfi S, Roitberg BZ, Brown WD, Holden JE, Pyzalski R, Taylor MD, Carvey P, Ling Z, Trono D, Hantraye P, Deglon N, Aebischer P. 2000. Neurodegeneration prevented by lentiviral vector delivery of GDNF in primate models of Parkinson's disease. Science 290:767-773.
28. Lattenmayer C, Trummer E, Schriebl K, Vorauer-Uhl K, Mueller D, Katinger H, Kunert R. 2007. Characterisation of recombinant CHO cell lines by investigation of protein productivities and genetic parameters. J Biotechnol 128:716-725. (Pubitemid 46341483)
29. Lever AM, Strappe PM, Zhao J. 2004. Lentiviral vectors. J Biomed Sci 11:439-449.
30. Ma D, Wells JW. 2004. Mathematical basis for the optimization of single cell-line screening on multi-well plates. Biotechnol Lett 26:1441-1446. (Pubitemid 41187382)
31. Massie B, Couture F, Lamoureux L, Mosser DD, Guilbault C, Jolicoeur P, Belanger F, Langelier Y. 1998. Inducible overexpression of a toxic protein by an adenovirus vector with a tetracycline-regulatable expression cassette. J Virol 72:2289-2296. (Pubitemid 28100788)
32. Meissner P, Pick H, Kulangara A, Chatellard P, Friedrich K, Wurm FM. 2001. Transient gene expression: Recombinant protein production with suspension-adapted HEK293-EBNA cells. Biotechnol Bioeng 75:197-203. (Pubitemid 32937290)
33. Miyake K, Miyake N, Shimada T. 2007. Development of targeted gene transfer into human primary T lymphocytes and macrophages using high-titer recombinant HIV vectors. J Biotechnol 129:532-538. (Pubitemid 46560151)
34. Montesino R, Garcia R, Quintero O, Cremata JA. 1998. Variation in N-linked oligosaccharide structures on heterologous proteins secreted by the methylotrophic yeast Pichia pastoris. Protein Expr Purif 14:197-207. (Pubitemid 28504165)
35. Mullick A, Xu Y, Warren R, Koutroumanis M, Guilbault C, Broussau S, Malenfant F, Bourget L, Lamoureux L, Lo R, Caron AW, Pilotte A, Massie B. 2006. The cumate gene-switch: A system for regulated expression in mammalian cells. BMC Biotechnol 6:43. (Pubitemid 44816923)
36. Naldini L, Blomer U, Gallay P, Ory D, Mulligan R, Gage FH, Verma IM, Trono D. 1996. In vivo gene delivery and stable transduction of nondividing cells by a lentiviral vector. Science 272:263-267.
37. Nesvizhskii AI, Keller A, Kolker E, Aebersold R. 2003. A statistical model for identifying proteins by tandem mass spectrometry. Anal Chem 75:4646-4658. (Pubitemid 37082259)
38. Ni Y, Sun S, Oparaocha I, Humeau L, Davis B, Cohen R, Binder G, Chang YN, Slepushkin V, Dropulic B. 2005. Generation of a packaging cell line for prolonged large-scale production of high-titer HIV-1-based lentiviral vector. J Gene Med 7:818-834. (Pubitemid 40872867)
39. O'Callaghan PM, James DC. 2008. Systems biotechnology of mammalian cell factories. Brief Funct Genomic Proteomic 7:95-110. (Pubitemid 351728951)
40. Palomares LA, Estrada-Mondaca S, Ramirez OT. 2004. Production of recombinant proteins: Challenges and solutions. Methods Mol Biol 267:15-52.
41. Pavlou AK, Reichert JM. 2004. Recombinant protein therapeutics - Success rates, market trends and values to 2010. Nat Biotechnol 22:1513-1519.
42. Pham PL, Perret S, Cass B, Carpentier E, St-Laurent G, Bisson L, Kamen A, Durocher Y. 2005. Transient gene expression in HEK293 cells: Peptone addition posttransfection improves recombinant protein synthesis. Biotechnol Bioeng 90:332-344. (Pubitemid 40779210)
43. Rosser MP, Xia W, Hartsell S, McCaman M, Zhu Y, Wang S, Harvey S, Bringmann P, Cobb RR. 2005. Transient transfection of CHO-K1-S using serum-free medium in suspension: A rapid mammalian protein expression system. Protein Expr Purif 40:237-243. (Pubitemid 40335905)
44. Sastry L, Xu Y, Johnson T, Desai K, Rissing D, Marsh J, Cornetta K. 2003. Certification assays for HIV-1-based vectors: Frequent passage of gag sequences without evidence of replication-competent viruses. Mol Ther 8:830-839. (Pubitemid 37527459)
45. Sheridan PL, Bodner M, Lynn A, Phuong TK, DePolo NJ, de la Vega DJ, Jr., O'Dea J, Nguyen K, McCormack JE, Driver DA, Townsend K, Ibanez CE, Sajjadi NC, Greengard JS, Moore MD, Respess J, Chang SM, Dubensky TW, Jr., Jolly DJ, Sauter SL. 2000. Generation of retroviral packaging and producer cell lines for large-scale vector production and clinical application: Improved safety and high titer. Mol Ther 2:262-275.
46. Shevchenko A, Wilm M, Vorm O, Mann M. 1996. Mass spectrometric sequencing of proteins silver-stained polyacrylamide gels. Anal Chem 68:850-858. (Pubitemid 26101214)
47. Sinn PL, Sauter SL, McCray PB, Jr. 2005. Gene therapy progress and prospects: Development of improved lentiviral and retroviral vectors-Design, biosafety, and production. Gene Ther 12:1089-1098.
48. Subramanian S, Srienc F. 1996. Quantitative analysis of transient gene expression in mammalian cells using the green fluorescent protein. J Biotechnol 49:137-151. (Pubitemid 26316249)
49. Tait AS, Brown CJ, Galbraith DJ, Hines MJ, Hoare M, Birch JR, James DC. 2004. Transient production of recombinant proteins by Chinese hamster ovary cells using polyethyleneimine/DNA complexes in combination with microtubule disrupting anti-mitotic agents. Biotechnol Bioeng 88:707-721. (Pubitemid 40775327)
50. Vigna E, Cavalieri S, Ailles L, Geuna M, Loew R, Bujard H, Naldini L. 2002. Robust and efficient regulation of transgene expression in vivo by improved tetracycline-dependent lentiviral vectors. Mol Ther 5:252-261. (Pubitemid 34211220)
51. Walsh G. 2006. Biopharmaceutical benchmarks 2006. Nat Biotechnol 24:769-776.
52. Wulhfard S, Tissot S, Bouchet S, Cevey J, De JM, Hacker DL, Wurm FM. 2008. Mild hypothermia improves transient gene expression yields several fold in Chinese hamster ovary cells. Biotechnol Prog 24:458-465. (Pubitemid 351520363)
53. Wurm FM. 2004. Production of recombinant protein therapeutics in cultivated mammalian cells. Nat Biotechnol 22:1393-1398. (Pubitemid 39482858)
54. Xu K, Ma H, McCown TJ, Verma IM, Kafri T. 2001. Generation of a stable cell line producing high-titer self-inactivating lentiviral vectors. Mol Ther 3:97-104. (Pubitemid 32509810)
55. Xu Y, Mullick A, Massie B. 2006. Expression system, components thereof and methods of use. Patent Appl. WO/2006/037215.
56. Zhang F, Thornhill SI, Howe SJ, Ulaganathan M, Schambach A, Sinclair J, Kinnon C, Gaspar HB, Antoniou M, Thrasher AJ. 2007. Lentiviral vectors containing an enhancer-less ubiquitously acting chromatin opening element (UCOE) provide highly reproducible and stable transgene expression in hematopoietic cells. Blood 110:1448-1457. (Pubitemid 47443959)