Accelerated homology-directed targeted integration of transgenes in Chinese hamster ovary cells via CRISPR/Cas9 and fluorescent enrichment

Biotechnology and Bioengineering

Volumn 113, Issue 11, 2016, Pages 2518-2523

  Lee, Jae Seong ^a   Grav, Lise Marie ^a   Pedersen, Lasse Ebdrup ^a   Lee, Gyun Min ^a,b   Kildegaard, Helene Faustrup ^a  

^a Chalmers University of Technology   (Denmark)

^b Korea Advanced Institute of Science and Technology (KAIST)   (South Korea)

Author keywords

Chinese hamster ovary cells; CRISPR Cas9; fluorescent enrichment; homology directed repair; targeted integration

Indexed keywords

CELL CULTURE; CELLS; CYTOLOGY; FLUORESCENCE; INTEGRATION; REPAIR;

ANTIBIOTIC SELECTIONS; CHEMICAL TREATMENTS; CHINESE HAMSTER OVARY CELLS; CRISPR/CAS9; EDITING TECHNOLOGY; FLUORESCENT MARKERS; PRODUCTION CELLS; SELECTION MARKER;

GENES;

ANTIBODY; GENOMIC DNA; PROTEIN;

ANIMAL CELL; ARTICLE; CHO CELL LINE; CONTROLLED STUDY; CRISPR CAS SYSTEM; CRISPR CAS9 SYSTEM; DNA END JOINING REPAIR; DNA INTEGRATION; FEMALE; FLUORESCENCE; GENOME; HOMOLOGY DIRECTED REPAIR TARGETED INTEGRATION; INDEL MUTATION; NONHUMAN; REAL TIME POLYMERASE CHAIN REACTION; RNA EDITING; TRANSGENE;

EID: 84988926749     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.26002     Document Type: Article

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