Induction of protein aggregation in an early secretory compartment by elevation of expression level

Biotechnology and Bioengineering

Volumn 78, Issue 2, 2002, Pages 131-140

  Schröder, Martin ^b   Schäfer, Rolf ^c   Friedl, Peter ^a  

^a DARMSTADT UNIVERSITY OF TECHNOLOGY   (Germany)

^b UNIVERSITY OF MICHIGAN HEALTH SYSTEM   (United States)

^c Xantos Biomedicine AG   (Germany)

Author keywords

Antithrombin III; Protein aggregation; Protein trafficking; Rate limiting step of protein secretion; Secretory pathway

Indexed keywords

AGGLOMERATION; ANIMAL CELL CULTURE; GLYCOLS; POLYPEPTIDES;

GLYCOSYLATION;

PROTEINS;

ANTITHROMBIN III; GLUCOSE; GLUTAMINE; GLYCOPROTEIN; RECOMBINANT PROTEIN;

AMINO ACID SEQUENCE; ANIMAL CELL; ARTICLE; CELL FRACTIONATION; CONTROLLED STUDY; DISULFIDE BOND; ENDOPLASMIC RETICULUM; EUKARYOTIC CELL; GOLGI COMPLEX; MAMMAL CELL; NONHUMAN; PROTEIN AGGREGATION; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN GLYCOSYLATION; PROTEIN SECRETION; PROTEIN STRUCTURE; PROTEIN TRANSPORT; SIALYLATION;

ANIMALS; ANTITHROMBIN III; BLOTTING, WESTERN; CELL DIVISION; CELLS, CULTURED; CHO CELLS; CRICETINAE; DISULFIDES; ENDOPLASMIC RETICULUM; ENZYME-LINKED IMMUNOSORBENT ASSAY; GLUCOSE; GLUTAMINE; GOLGI APPARATUS; HEXOSAMINIDASES; HUMANS; LACTIC ACID; MANNOSYL-GLYCOPROTEIN ENDO-BETA-N-ACETYLGLUCOSAMINIDASE; MOLECULAR WEIGHT; PROTEIN CONFORMATION; QUATERNARY AMMONIUM COMPOUNDS; RECOMBINANT PROTEINS;

CRICETINAE; CRICETULUS GRISEUS; EUKARYOTA; MAMMALIA;

EID: 0037140419     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.10206     Document Type: Article

References (61)

1. Distribution of protein disulfide isomerase in rate hepatocytes
2. The lectin ERGIC-53 is a cargo transport receptor for glycoproteins
3. Role of ATP and disulphide bonds during protein folding in the endoplasmic reticulum
4. Expression of human antithrombin III in Saccharomyces cerevisiae and Schizosaccharomyces pombe
5. High level expression of tissue inhibitor of metalloproteinases in Chinese hamster ovary cells using glutamine synthetase gene amplification
6. Mechanisms and catalysts of disulphide bond formation in proteins
7. Posttranslational folding of vesicular stomatitis virus G protein in the ER: Involvement of noncovalent and covalent complexes
8. Structural studies on the carbohydrate portion of human antithrombin III
9. Protein disulfide isomerase: Multiple roles in the modification of nascent secretory proteins
10. Role of protein disulphide-isomerase in the expression of native proteins
11. Protein disulfide isomerase: Building bridges in protein folding
12. A regulatory link between ER-associated protein degradation and the unfolded-protein response
13. One-dimensional SDS gel electrophoresis of proteins
14. The nonglycosylated glycoprotein of vesicular stomatitis virus is temperature-sensitive and undergoes intracellular aggregation at elevated temperatures
15. The preparation of subcellular organelles from mouse-liver in self-generated gradients
16. 1-antitrypsin within the hepatic endoplasmic reticulum does not elevate the steady-state level of grp78/BiP
17. Protein folding in the cell
18. Trans-activated expression of fully gamma-carboxylated recombinant human-protein C, an antithrombotic factor
19. Quality control in the secretory pathway: Retention of a misfolded glycoprotein involves cycling between the ER, intermediate compartment, and Golgi apparatus
20. Protein translation and folding are coupled by an endoplasmic-reticulum resident kinase
21. Lectins and traffic in the secretory pathway
22. Interactions of misfolded influenza virus hemagglutinin with binding protein (BiP)
23. Stress signaling from the lumen of the endoplasmic reticulum: Coordination of gene transcriptional and translational controls
24. Synthesis, processing and secretion of recombinant human factor VIII expressed in mammalian cells
25. Expression, purification and characterization of recombinant γ-carboxylated factor IX synthesized in Chinese hamster ovary cells
26. Protein aggregation in vitro and in vivo: A quantitative model of the kinetic competition between folding and aggregation
27. Assembly of asparagine-linked oligosaccharides
28. D-glucose. Colorimetric methods with glucose oxidase and peroxidase
29. 3H]glucosamine incorporation into yeast glycoproteins: Binding of tunicamycin and interaction with phospholipids
30. Biochemical determinants of clearance of tissue-type plasminogen activator from the circulation
31. Heavy chain binding protein recognizes incompletely disulfide-bonded forms of vesicular stomatitis virus G protein
32. Vesicular stomatitis virus G proteins with altered glycosylation sites display temperature-sensitive intracellular transport and are subject to aberrant intermolecular disulfide bonding
33. Misfolding and aggregation of newly synthesized proteins in the endoplasmic reticulum
34. Glutaminolysis in animal cells
35. Characterization of a bifunctional peptidylglycine α-amidating enzyme expressed in Chinese hamster ovary cells
36. Structural studies of the carbohydrate moiety of human antithrombin III
37. Routine subculturing - Adherent cells
38. Glycosidases of the asparagine-linked oligosaccharide processing pathway
39. Mutations in the ER-Golgi intermediate compartment protein ERGIC-53 cause combined deficiency of coagulation factors V and VIII
40. The size and shape of human and bovine antithrombin III
41. Association of folding intermediates of glycoproteins with calnexin during protein maturation
42. N-glycosylation and the production of recombinant glycoproteins
43. Effect of cloned gene dosage on cell growth and hepatitis B surface antigen synthesis and secretion in recombinant CHO cells
44. 1-antitrypsin and antithrombin III
45. Isolation of a cDNA clone for human antithrombin III
46. Metabolic rates of vascular endothelial cells in vitro
47. Overexpression of recombinant human antithrombin III in Chinese hamster ovary cells results in malformation and decreased secretion of the recombinant protein
48. Indirect sandwich ELISA for human antithrombin III employing the interaction between D-biotin and streptavidin
49. Quantitative analysis of transcription and translation in gene amplified Chinese hamster ovary cells on the basis of a kinetic model
50. Spectrophotometric determination of iodixanol in subcellular fractions of mammalian cells
51. Intracellular transport of soluble and membrane-bound glycoproteins: Folding, assembly and secretion of anchor-free influenza hemagglutinin
52. Isolation of subcellular organelles
53. Vitamin K-dependent carboxylase
54. Isolation of Chinese hamster cell mutants deficient in dihydrofolate reductase activity
55. Effect of ATP depletion and DTT on the transport of membrane proteins from the endoplasmic reticulum and the intermediate compartment to the Golgi complex
56. Hemocytometer cell counts and viability studies
57. The rate of bulk flow from the endoplasmic reticulum to the cell surface
58. High level synthesis of immunoglobulins in Chinese hamster ovary cells
59. Production of recombinant proteins in Chinese hamster ovary cells using a protein-free cell culture medium
60. Characterization of recombinant human antithrombin III synthesized in Chinese hamster ovary cells