Combined in silico modeling and metabolomics analysis to characterize fed-batch CHO cell culture

Biotechnology and Bioengineering

Volumn 109, Issue 6, 2012, Pages 1415-1429

  Selvarasu, Suresh ^a   Ho, Ying Swan ^a   Chong, William P K ^a   Wong, Niki S C ^a   Yusufi, Faraaz N K ^a   Lee, Yih Yean ^a   Yap, Miranda G S ^a,b   Lee, Dong Yup ^a,b  

^a AGENCY FOR SCIENCE   (Singapore)

^b NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

Author keywords

CHO cells; Constraints based flux analysis; Genome scale metabolic model; Mammalian systems biotechnology; Metabolomics

Indexed keywords

CHO CELL; FLUX ANALYSIS; GENOME-SCALE METABOLIC MODELS; MAMMALIAN SYSTEMS; METABOLOMICS;

AMINO ACIDS; ANIMAL CELL CULTURE; BIOINFORMATICS; BIOMOLECULES; CELL GROWTH; ENZYME ACTIVITY; GROWTH KINETICS; MAMMALS; METABOLISM; METABOLITES; RECOMBINANT PROTEINS;

BATCH CELL CULTURE;

GLUTATHIONE; GLYCEROPHOSPHOLIPID;

ANIMAL CELL; ANIMAL CELL CULTURE; ARTICLE; BIOMASS; BIOREACTOR; CELL DENSITY; CELL GROWTH; COMPUTER MODEL; CONTROLLED STUDY; CYTOLOGY; ENERGY; EXTRACELLULAR SPACE; FED BATCH CULTURE; GENE EXPRESSION PROFILING; GENOMICS; INTRACELLULAR MEMBRANE; LIPID METABOLISM; METABOLOMICS; NONHUMAN; OXIDATIVE STRESS; PHYSIOLOGY; PROTEIN ANALYSIS; SIGNAL TRANSDUCTION;

ANIMALS; CELL CULTURE TECHNIQUES; CHO CELLS; COMPUTER SIMULATION; CRICETINAE; CRICETULUS; CULTURE MEDIA; EPITHELIAL CELLS; METABOLOME;

CRICETULUS GRISEUS; MAMMALIA;

EID: 84862784156     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.24445     Document Type: Article

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