Adaptation of mammalian cells to growth in serum-free media

Applied Biochemistry and Biotechnology - Part B Molecular Biotechnology

Volumn 15, Issue 3, 2000, Pages 249-257

  Sinacore, M S ^a   Drapeau, D ^a   Adamson, S R ^a  

^a Genetics Institute Cambridge   (United States)

Author keywords

Adaptation; Biopharmaceutical; Bioreactor; Mammalian cell culture; Serum free medium; Suspension culture

Indexed keywords

ADAPTATION; ANIMAL CELL; ARTICLE; BIOREACTOR; CELL CULTURE; CELL GROWTH; CHO CELL; CULTURE MEDIUM; MAMMAL CELL; NONHUMAN; NUTRIENT;

ANIMALIA; MAMMALIA;

EID: 0033846848     PISSN: 10736085     EISSN: None     Source Type: Journal    
DOI: 10.1385/mb:15:3:249     Document Type: Article

References (36)

1. Transient responses of hybridoma cells to lactate and ammonia pulse and step changes in continuous culture
2. Adaptation of hybridoma cells to higher ammonia concentration
3. Growth study of lactate and ammonia double-resistant clones of HL-60 cells
4. Chinese hamster ovary cell growth and interferon production kinetics in stirred batch culture
5. Large-scale production of erythroid differentiation factor (EDF) by gene-engineered Chinese hamster ovary (CHO) cells in suspension culture
6. High-level expression of secreted proteins from cells adapted to serum-free suspension culture
7. Production of recombinant proteins in serum-free media
8. Optimizing cell and culture environment for production of recombinant proteins
9. Development of a serum-free culture medium for the large scale production of recombinant protein from a Chinese hamster ovary cell line
10. An experimental rabies vaccine produced with a new BHK-21 suspension cell culture process: Use of serum-free medium and perfusion-reactor system
11. CHO DUKX cell lineages preadapted to growth in serum-free suspension culture enable rapid development of cell culture processes for the manufacture of recombinant proteins
12. Production of recombinant proteins in Chinese hamster ovary cells using protein-free cell culture medium
13. Amplification and expression of recombinant genes in serum-independent Chinese hamster ovary cells
14. Development of a serum-free medium for growth of NS-1 mouse myeloma cells and its application to the isolation of NS-1 hybridomas
15. Serum-free medium for hybridoma and parental myeloma cell cultivation: A novel composition of growth-supporting substances
16. Expression of human beta-interferon in Namalwa KJM-1 which was adapted to serum-free medium
17. Cultural and physiological factors affecting expression of recombinant proteins
18. Anchorage-dependent cell cycle progression
19. Anchorage dependence, integrins and apoptosis
20. Serum withdrawal induced apoptotic cell death in K1-Ras transformed but not normal differentiated thyroid cells
21. Recombinant human interferon-γ: Differences in glycosylation and proteolytic processing lead to heterogeneity in batch culture
22. Comparison of N-linked oligosaccharides of recombinant human tissue kallikrein produced by Chinese hamster ovary cells in microcarriers and in serum-free suspension culture
23. Role of environmental conditions on the expression levels, glycoform pattern and levels of sialyltransferase for hFSH produced by recombinant CHO cells
24. Glycosylation and biological activity of CAMPATH-1H expressed in different cell lines and grown under different culture conditions
25. Getting the glycosylation right: Implications for the biotechnology industry
26. Characterization of changes in the glycosylation pattern of recombinant proteins from BHK-21 cells due to different culture conditions
27. Physiological changes during the adaptation of hybridoma cells to low serum and serum-free media
28. Methods for growth of cultured cells in serum-free medium
29. Evaluation of the new serum-free medium (MDSS2) for the production of different biologicals: Use of various cell lines
30. Weaning of three hybridoma cell lines to serum free low protein medium
31. Evaluation of a simple protein free medium that supports high levels of monoclonal antibody production
32. Production of monoclonal antibodies in serum-free medium
33. A simple serum-free freezing medium for serum-free cultured cells