Model-directed engineering of "difficult-to-express" monoclonal antibody production by Chinese hamster ovary cells

Biotechnology and Bioengineering

Volumn 111, Issue 2, 2014, Pages 372-385

  Pybus, Leon P ^a   Dean, Greg ^b   West, Nathan R ^a   Smith, Andrew ^b   Daramola, Olalekan ^b   Field, Ray ^b   Wilkinson, Stephen J ^a   James, David C ^a  

^a UNIVERSITY OF SHEFFIELD   (United Kingdom)

^b MEDIMMUNE   (United States)

Author keywords

Cell line engineering; Chinese hamster ovary cells; Difficult to express proteins; Mathematical modeling; Recombinant monoclonal antibody; Unfolded protein response

Indexed keywords

CELL LINES; CHINESE HAMSTER OVARY CELLS; INCREASE PRODUCTIONS; MOLECULAR CHAPERONES; MONOCLONAL ANTIBODIES (MAB); MONOCLONAL ANTIBODY PRODUCTION; RECOMBINANT MONOCLONAL ANTIBODY; UNFOLDED PROTEIN RESPONSE;

BIOSYNTHESIS; CELL CULTURE; CELL GROWTH; CELLS; ENGINEERING; GROWTH KINETICS; MATHEMATICAL MODELS; MONOCLONAL ANTIBODIES; RECOMBINANT PROTEINS;

CELL ENGINEERING;

IMMUNOGLOBULIN G1 LAMBDA; IMMUNOGLOBULIN LAMBDA CHAIN; MONOCLONAL ANTIBODY; UNCLASSIFIED DRUG; CHAPERONE; RECOMBINANT PROTEIN;

ANIMAL CELL; ANTIBODY PRODUCTION; ANTIBODY TITER; ARTICLE; BIOENGINEERING; CELL ENGINEERING; CELL GROWTH; CHO CELL; CONTROLLED STUDY; FEMALE; GROWTH INHIBITION; NONHUMAN; UNFOLDED PROTEIN RESPONSE; VOLUMETRY; ANIMAL; BIOSYNTHESIS; BIOTECHNOLOGY; CELL PROLIFERATION; CHO CELL LINE; CRICETULUS; GENETICS; METABOLIC ENGINEERING; METABOLISM; MOLECULAR BIOLOGY; PHARMACEUTICS; PROCEDURES; PROTEIN FOLDING;

CRICETULUS GRISEUS; MAMMALIA;

ANIMALS; ANTIBODIES, MONOCLONAL; BIOTECHNOLOGY; CELL PROLIFERATION; CHO CELLS; CRICETULUS; METABOLIC ENGINEERING; MOLECULAR BIOLOGY; MOLECULAR CHAPERONES; PROTEIN FOLDING; RECOMBINANT PROTEINS; TECHNOLOGY, PHARMACEUTICAL; UNFOLDED PROTEIN RESPONSE;

EID: 84890800315     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.25116     Document Type: Article

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