Use of an anti-apoptotic CHO cell line for transient gene expression

Biotechnology Progress

Volumn 29, Issue 4, 2013, Pages 1050-1058

  Macaraeg, Nichole F ^a   Reilly, Dorothea E ^a   Wong, Athena W ^a  

^a GENENTECH INC   (United States)

Author keywords

Apoptosis; Bak; Bax; Endocytosis; Transfection efficiency; Transient gene expression

Indexed keywords

BAK; BAX; ENDOCYTOSIS; TRANSFECTION EFFICIENCY; TRANSIENT GENE EXPRESSIONS;

CELL CULTURE; CELL DEATH; CELL MEMBRANES; DNA; GENE EXPRESSION;

MOLECULAR BIOLOGY;

MAMMALIA;

DNA; MESSENGER RNA;

ANIMAL; APOPTOSIS; ARTICLE; BAK; BAX; CELL CULTURE; CHO CELL LINE; CRICETULUS; ENDOCYTOSIS; GENE EXPRESSION; GENETICS; ISOLATION AND PURIFICATION; TRANSFECTION EFFICIENCY; TRANSIENT EXPRESSION;

APOPTOSIS; BAK; BAX; ENDOCYTOSIS; TRANSFECTION EFFICIENCY; TRANSIENT GENE EXPRESSION;

ANIMALS; APOPTOSIS; CELLS, CULTURED; CHO CELLS; CRICETULUS; DNA; GENE EXPRESSION; RNA, MESSENGER;

EID: 84881615449     PISSN: 87567938     EISSN: 15206033     Source Type: Journal    
DOI: 10.1002/btpr.1763     Document Type: Article

References (42)

1. Baldi L, Hacker DL, Adam M, Wurm FM. Recombinant protein production by large-scale transient gene expression in mammalian cells: state of the art and future perspectives. Biotechnol Lett. 2007;29:677-684.
2. Geisse S, Fux C. Recombinant protein production by transient gene transfer into Mammalian cells. Methods Enzymol. 2009;463:223-238.
3. Girard P, Derouazi M, Baumgartner G, Bourgeois M, Jordan M, Jacko B, Wurm FM. 100-Liter transient transfection. Cytotechnology. 2002;38:15-21.
4. Rajendra Y, Kiseljak D, Baldi L, Hacker DL, Wurm FM. A simple high-yielding process for transient gene expression in CHO cells. J Biotechnol. 2011;153:22-26.
5. De Jesus M, Wurm FM. Manufacturing recombinant proteins in kg-ton quantities using animal cells in bioreactors. Eur J Pharm Biopharm. 2011;78:184-188.
6. Kim JY, Kim YG, Lee GM. CHO cells in biotechnology for production of recombinant proteins: current state and further potential. Appl Microbiol Biotechnol. 2012;93:917-930.
7. Chisti Y. Animal-cell damage in sparged bioreactors. Trends Biotechnol. 2000;18:420-432.
8. Mercille S, Massie B. Induction of apoptosis in nutrient-deprived cultures of hybridoma and myeloma cells. Biotechnol Bioeng. 1994;44:1140-1154.
9. Ryu JS, Lee GM. Application of hypoosmolar medium to fed-batch culture of hybridoma cells for improvement of culture longevity. Biotechnol Bioeng. 1999;62:120-123.
10. Goswami J, Sinskey AJ, Steller H, Stephanopoulos GN, Wang DI. Apoptosis in batch cultures of Chinese hamster ovary cells. Biotechnol Bioeng. 1999;62:632-640.
11. Tait SW, Green DR. Mitochondria and cell death: outer membrane permeabilization and beyond. Nat Rev Mol Cell Biol. 2010;11:621-632.
12. Cory S, Adams JM. The Bcl2 family: regulators of the cellular life-or-death switch. Nat Rev Cancer. 2002;2:647-656.
13. Lim SF, Chuan KH, Liu S, Loh SO, Chung BY, Ong CC, Song Z. RNAi suppression of Bax and Bak enhances viability in fed-batch cultures of CHO cells. Metab Eng. 2006;8:509-522.
14. Cost GJ, Freyvert Y, Vafiadis A, Santiago Y, Miller JC, Rebar E, Collingwood TN, Snowden A, Gregory PD. BAK and BAX deletion using zinc-finger nucleases yields apoptosis-resistant CHO cells. Biotechnol Bioeng. 2010;105:330-340.
15. Wong AW, Scales SJ, Reilly DE. DNA internalized via caveolae requires microtubule-dependent, Rab7-independent transport to the late endocytic pathway for delivery to the nucleus. J Biol Chem. 2007;282:22953-22963.
16. Cohen RN, van der Aa MA, Macaraeg N, Lee AP, Szoka FC, Jr. Quantification of plasmid DNA copies in the nucleus after lipoplex and polyplex transfection. J Control Release. 2009;135:166-174.
17. Lewin B. Genes VI. Oxford: Oxford University Press:; 1997. p xviii, 1260 p.
18. Mennesson E, Erbacher P, Piller V, Kieda C, Midoux P, Pichon C. Transfection efficiency and uptake process of polyplexes in human lung endothelial cells: a comparative study in non-polarized and polarized cells. J Gene Med. 2005;7:729-738.
19. Derouazi M, Girard P, Van Tilborgh F, Iglesias K, Muller N, Bertschinger M, Wurm FM. Serum-free large-scale transient transfection of CHO cells. Biotechnol Bioeng. 2004;87:537-545.
20. Bertschinger M, Schertenleib A, Cevey J, Hacker D. L, Wurm FM. The kinetics of polyethylenimine-mediated transfection in suspension cultures of Chinese hamster ovary cells. Mol Biotechnol. 2008;40:136-143.
21. Choosakoonkriang S, Lobo BA, Koe GS, Koe JG, Middaugh CR. Biophysical characterization of PEI/DNA complexes. J Pharm Sci. 2003;92:1710-1722.
22. Dorai H, Kyung YS, Ellis D, Kinney C, Lin C, Jan D, Moore G, Betenbaugh MJ. Expression of anti-apoptosis genes alters lactate metabolism of Chinese Hamster Ovary cells in culture. Biotechnol Bioeng. 2009;103:592-608.
23. Lao MS, Toth D. Effects of ammonium and lactate on growth and metabolism of a recombinant Chinese hamster ovary cell culture. Biotechnol Prog. 1997;13:688-691.
24. Sureshkumar GK, Mutharasan R. The influence of temperature on a mouse-mouse hybridoma growth and monoclonal antibody production. Biotechnol Bioeng. 1991;37:292-295.
25. Reuveny S, Velez D, Macmillan JD, Miller L. Factors affecting cell growth and monoclonal antibody production in stirred reactors. J Immunol Methods. 1986;86:53-59.
26. Stettler M, Jaccard N, Hacker D, De Jesus M, Wurm FM, Jordan M. New disposable tubes for rapid and precise biomass assessment for suspension cultures of mammalian cells. Biotechnol Bioeng. 2006;95:1228-1233.
27. Riedl SJ, Shi Y. Molecular mechanisms of caspase regulation during apoptosis. Nat Rev Mol Cell Biol. 2004;5:897-907.
28. Cohen GM. Caspases: the executioners of apoptosis. Biochem J. 1997;326:1-16.
29. Godbey WT, Wu KK, Mikos AG. Poly(ethylenimine) and its role in gene delivery. J Control Release. 1999;60:149-160.
30. Antonsson A, Persson JL. Induction of apoptosis by staurosporine involves the inhibition of expression of the major cell cycle proteins at the G(2)/m checkpoint accompanied by alterations in Erk and Akt kinase activities. Anticancer Res. 2009;29:2893-2898.
31. Paris S, Burlacu A, Durocher Y. Opposing roles of syndecan-1 and syndecan-2 in polyethyleneimine-mediated gene delivery. J Biol Chem. 2008;283:7697-7704.
32. Kopatz I, Remy JS, Behr JP. A model for non-viral gene delivery: through syndecan adhesion molecules and powered by actin. J Gene Med. 2004;6:769-776.
33. Ruponen M, Ronkko S, Honkakoski P, Pelkonen J, Tammi M, Urtti A. Extracellular glycosaminoglycans modify cellular trafficking of lipoplexes and polyplexes. J Biol Chem. 2001;276:33875-33880.
34. Wong AW, Baginski TK, Reilly DE. Enhancement of DNA uptake in FUT8-deleted CHO cells for transient production of afucosylated antibodies. Biotechnol Bioeng. 2010;106:751-763.
35. Zuhorn IS, Engberts JB, Hoekstra D. Gene delivery by cationic lipid vectors: overcoming cellular barriers. Eur Biophys J. 2007;36:349-362.
36. Khalil IA, Kogure K, Akita H, Harashima H. Uptake pathways and subsequent intracellular trafficking in nonviral gene delivery. Pharmacol Rev. 2006;58:32-45.
37. Conner SD, Schmid SL. Regulated portals of entry into the cell. Nature 2003;422:37-44.
38. Mayor S, Pagano RE. Pathways of clathrin-independent endocytosis. Nat Rev Mol Cell Biol. 2007;8:603-612.
39. Xie Q, Xinyong G, Xianjin C, Yayu W. PEI/DNA formation affects transient gene expression in suspension Chinese hamster ovary cells via a one-step transfection process. Cytotechnology. 2012;65:263-271.
40. Zhao QQ, Chen JL, Lv TF, He CX, Tang GP, Liang WQ, Tabata Y, Gao JQ. N/P ratio significantly influences the transfection efficiency and cytotoxicity of a polyethylenimine/chitosan/DNA complex. Biol Pharm Bull. 2009;32:706-710.
41. Misaghi S, Qu Y, Snowden A, Chang J, Snedecor B. Resilient immortals, characterizing and utilizing Bax/Bak deficient Chinese hamster ovary (CHO) cells for high titer antibody production. Biotech Progress. 2013 Apr 18.
42. Pichler J, Galosy S, Mott J, Borth N. Selection of CHO host cell subclones with increased specific antibody production rates by repeated cycles of transient transfection and cell sorting. Biotechnol Bioeng. 2011;108:386-394.