Site-specific integration in CHO cells mediated by CRISPR/Cas9 and homology-directed DNA repair pathway

Scientific Reports

Volumn 5, Issue , 2015, Pages

  Lee, Jae Seong ^a   Kallehauge, Thomas Beuchert ^a   Pedersen, Lasse Ebdrup ^a   Kildegaard, Helene Faustrup ^a  

^a Chalmers University of Technology   (Denmark)

Author keywords

[No Author keywords available]

Indexed keywords

ACYLTRANSFERASE; CHAPERONE;

ANIMAL; CHO CELL LINE; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; CRICETULUS; DEFICIENCY; DNA REPAIR; GENE LOCUS; GENE TARGETING; GENE VECTOR; GENETICS; HAMSTER; METABOLISM; NUCLEOTIDE SEQUENCE; PROCEDURES;

ACYLTRANSFERASES; ANIMALS; BASE SEQUENCE; CHO CELLS; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEATS; CRICETINAE; CRICETULUS; DNA REPAIR; GENE TARGETING; GENETIC LOCI; GENETIC VECTORS; MOLECULAR CHAPERONES;

EID: 84923667065     PISSN: None     EISSN: 20452322     Source Type: Journal    
DOI: 10.1038/srep08572     Document Type: Article

References (37)

1. Noh, S. M., Sathyamurthy, M. & Lee, G. M. Development of recombinant Chinese hamster ovary cell lines for therapeutic protein production. Curr. Opin. Chem. Eng. 2, 391-397 (2013).
2. Wurm, F. M. Production of recombinant protein therapeutics in cultivated mammalian cells. Nat. Biotechnol. 22, 1393-1398 (2004).
3. Wilson, C., Bellen, H. J. & Gehring, W. J. Position effects on eukaryotic gene expression. Annu. Rev. Cell Biol. 6, 679-714 (1990).
4. Kim, S. J., Kim, N. S., Ryu, C. J., Hong, H. J. & Lee, G. M. Characterization of chimeric antibody producing CHO cells in the course of dihydrofolate reductase-mediated gene amplification and their stability in the absence of selective pressure. Biotechnol. Bioeng. 58, 73-84 (1998).
5. Huang, Y. et al. An efficient and targeted gene integration system for high-level antibody expression. J. Immunol. Methods 322, 28-39 (2007).
6. Kito, M., Itami, S., Fukano, Y., Yamana, K. & Shibui, T. Construction of engineered CHO strains for high-level production of recombinant proteins. Appl. Microbiol. Biotechnol. 60, 442-448 (2002).
7. Lieu, P. T. et al. Generation of site-specific retargeting platform cell lines for drug discovery using phiC31 and R4 integrases. J. Biomol. Screen. 14, 1207-1215 (2009).
8. Lewis, N. E. et al. Genomic landscapes of Chinese hamster ovary cell lines as revealed by the Cricetulus griseus draft genome. Nat. Biotechnol. 31, 759-765 (2013).
9. Xu, X. et al. The genomic sequence of the Chinese hamster ovary (CHO)-K1 cell line. Nat. Biotechnol. 29, 735-741 (2011).
10. Carroll, D. Genome engineering with targetable nucleases. Annu. Rev. Biochem. 83, 409-439 (2014).
11. Cong, L. et al . Multiplex Genome Engineering Using CRISPR/Cas Systems. Science 339, 819-823 (2013).
12. Hockemeyer, D. et al. Efficient targeting of expressed and silent genes in human ESCs and iPSCs using zinc-finger nucleases. Nat. Biotechnol. 27, 851-857 (2009).
13. Lombardo, A. et al. Site-specific integration and tailoring of cassette design for sustainable gene transfer. Nat. Methods 8, 861-869 (2011).
14. Mali, P. et al. RNA-guided human genome engineering via Cas9. Science 339, 823-826 (2013).
15. Miller, J. C. et al. A TALE nuclease architecture for efficient genome editing. Nat. Biotechnol. 29, 143-148 (2011).
16. Orlando, S. J. et al. Zinc-finger nuclease-driven targeted integration into mammalian genomes using donors with limited chromosomal homology. Nucleic Acids Res. 38, e152 (2010).
17. Cristea, S. et al. In vivo cleavage of transgene donors promotes nuclease-mediated targeted integration. Biotechnol. Bioeng. 110, 871-880 (2013).
18. Pennisi, E. The CRISPR craze. Science 341, 833-836 (2013).
19. Jinek, M. et al. A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. Science 337, 816-821 (2012).
20. Ronda, C. et al. Accelerating genome editing in CHO cells using CRISPR Cas9 and CRISPy, a web-based target finding tool. Biotechnol. Bioeng. 111, 1604-1616 (2014).
21. Chen, W. & Stanley, P. Five Lec1 CHO cell mutants have distinct Mgat1 gene mutations that encode truncated N-acetylglucosaminyltransferase I. Glycobiology 13, 43-50 (2003).
22. Sealover, N. R. et al. Engineering Chinese hamster ovary (CHO) cells for producing recombinant proteins with simple glycoforms by zinc-finger nuclease (ZFN)-mediated gene knockout of mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase (Mgat1). J. Biotechnol. 167, 24-32 (2013).
23. Doench, J. G. et al. Rational design of highly active sgRNAs for CRISPR-Cas9-mediated gene inactivation. Nat. Biotechnol. doi: 10.1038/nbt.3026. [Epub ahead of print] (2014).
24. Ramakrishna, S. et al. Surrogate reporter-based enrichment of cells containing RNA-guided Cas9 nuclease-induced mutations. Nat. Commun. 5, 3378 (2014).
25. Vriend, L. E., Jasin, M. & Krawczyk, P. M. Assaying Break and Nick-Induced Homologous Recombination in Mammalian Cells Using the DR-GFP Reporter and Cas9 Nucleases. Methods Enzymol. 546, 175-191 (2014).
26. Ran, F. A. et al. Double nicking by RNA-guided CRISPR Cas9 for enhanced genome editing specificity. Cell 154, 1380-1389 (2013).
27. Fu, Y. et al. High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells. Nat. Biotechnol. 31, 822-826 (2013).
28. Hsu, P. D. et al. DNA targeting specificity of RNA-guided Cas9 nucleases. Nat. Biotechnol. 31, 827-832 (2013).
29. Mali, P. et al. CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering. Nat. Biotechnol. 31, 833-838 (2013).
30. Wu, X. et al. Genome-wide binding of the CRISPR endonuclease Cas9 in mammalian cells. Nat. Biotechnol. 32, 670-676 (2014).
31. Lin, Y. et al. CRISPR/Cas9 systems have off-target activity with insertions or deletions between target DNA and guide RNA sequences. Nucleic Acids Res. 42, 7473-7485 (2014).
32. Fu, Y., Sander, J. D., Reyon, D., Cascio, V. M. & Joung, J. K. Improving CRISPRC-as nuclease specificity using truncated guide RNAs. Nat. Biotechnol. 32, 279-284 (2014).
33. Tsai, S. Q. et al. Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing. Nat. Biotechnol. 32, 569-576 (2014).
34. Papapetrou, E. P. et al. Genomic safe harbors permit high b-globin transgene expression in thalassemia induced pluripotent stem cells. Nat. Biotechnol. 29, 73-78 (2011).
35. Aymard, F. et al. Transcriptionally active chromatin recruits homologous recombination at DNA double-strand breaks. Nat. Struct. Mol. Biol. 21, 366-374 (2014).
36. Lund, A. M. et al. A Versatile System for USER Cloning-Based Assembly of Expression Vectors for Mammalian Cell Engineering. PLoS One 9, e96693 (2014).
37. Livak, K. J. & Schmittgen, T. D. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods 25, 402-408 (2001).