Fusion tags for protein solubility, purification, and immunogenicity in Escherichia coli: The novel Fh8 system

Frontiers in Microbiology

Volumn 5, Issue FEB, 2014, Pages

  Costa, Sofia ^a,b   Almeida, André ^c   Castro, António ^b   Domingues, Lucília ^a  

^a UNIVERSITY OF MINHO   (Portugal)

^b NATIONAL INSTITUTE OF HEALTH   (Portugal)

^c Parque Technologico de Cantanhede ^*  (Portugal)

Author keywords

Escherichia coli; Fh8 tag; Fusion tags; H tag; Protein immunogenicity; Protein purification; Soluble production; Tag removal

Indexed keywords

ARSENATE REDUCTASE; BACTERIAL PROTEIN; CALCIUM BINDING PROTEIN; ESCHERICHIA COLI SECRETED PROTEIN A; GLUTATHIONE TRANSFERASE; GREEN FLUORESCENT PROTEIN; HEAT SHOCK PROTEIN; HISTIDINE; HYBRID PROTEIN; INITIATION FACTOR; MALTOSE BINDING PROTEIN; RECOMBINANT PROTEIN; SUPEROXIDE DISMUTASE; THIOREDOXIN;

AFFINITY CHROMATOGRAPHY; ENHANCER REGION; ESCHERICHIA COLI; FUSION GENE; GEL PERMEATION CHROMATOGRAPHY; GENE EXPRESSION SYSTEM; GENE FUSION; HUMAN; HYDROPHOBIC INTERACTION CHROMATOGRAPHY; IMMUNIZATION; IMMUNOGENICITY; ION EXCHANGE CHROMATOGRAPHY; NONHUMAN; PLASMODIUM FALCIPARUM; PROTEIN ENGINEERING; PROTEIN PURIFICATION; REVIEW;

EID: 84897604263     PISSN: None     EISSN: 1664302X     Source Type: Journal    
DOI: 10.3389/fmicb.2014.00063     Document Type: Review

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