CDNA Cloning and Functional Expression of the α-d-Galactose-Binding Lectin Frutalin in Escherichia coli

Molecular Biotechnology

Volumn 43, Issue 3, 2009, Pages 212-220

  Oliveira, Carla ^a   Costa, Sofia ^a   Teixeira, José A ^a   Domingues, Lucília ^a  

^a UNIVERSITY OF MINHO   (Portugal)

Author keywords

Escherichia coli expression system; Experimental factorial design; Frutalin cDNA cloning; Galactose binding jacalin related lectin; Hemagglutination activity

Indexed keywords

AMINO ACID SEQUENCE; BINDING LECTIN; BINDING SPECIFICITIES; CDNA CLONES; CDNA CLONING; CONCENTRATION OF; CULTURE CONDITIONS; D-GALACTOSE; DATABASE SEARCHES; EXPERIMENTAL FACTORIAL DESIGN; EXPRESSION VECTORS; FRUTALIN CDNA CLONING; FUNCTIONAL EXPRESSION; GALACTOSE-BINDING JACALIN-RELATED LECTIN; HEMAGGLUTINATION ACTIVITY; INSOLUBLE PROTEINS; JACALIN; SDS-PAGE; SOLUBLE EXPRESSION; WESTERN-BLOT ANALYSIS;

AMINES; AMINO ACIDS; ANTIGEN-ANTIBODY REACTIONS; BACTERIA; BACTERIOLOGY; CARBOHYDRATES; CLONING; DIFFUSERS (OPTICAL); ORGANIC ACIDS; SEARCH ENGINES;

ESCHERICHIA COLI;

COMPLEMENTARY DNA; FRUTALIN; JACALIN; LECTIN; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; BREADFRUIT; ESCHERICHIA COLI; GENE AMPLIFICATION; LECTIN BINDING; MOLECULAR CLONING; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN SYNTHESIS;

AMINO ACID SEQUENCE; ARTOCARPUS; CARBOHYDRATES; CHROMATOGRAPHY, GEL; CLONING, MOLECULAR; DATA INTERPRETATION, STATISTICAL; DNA, COMPLEMENTARY; ESCHERICHIA COLI; HEMAGGLUTINATION TESTS; ISOPROPYL THIOGALACTOSIDE; MOLECULAR SEQUENCE DATA; PLANT LECTINS; RECOMBINANT PROTEINS; SEQUENCE ALIGNMENT; SOLUBILITY; TEMPERATURE; TIME FACTORS;

ARTOCARPUS ALTILIS; ARTOCARPUS INCISA; ESCHERICHIA COLI;

EID: 70350567797     PISSN: 10736085     EISSN: None     Source Type: Journal    
DOI: 10.1007/s12033-009-9191-7     Document Type: Article

References (28)

1. WJ Peumans EJM Van Damme 1995 Lectins as plant defense proteins Plant Physiology 109 347 352 10.1104/pp.109.2.347 10.1104/pp.109.2.347 1:CAS:528:DyaK2MXoslOqsLg%3D
2. NR Chandra N Kumar J Jeyakani DD Singh SB Gowda MN Prathima 2006 Lectindb: a plant lectin database Glycobiology 16 938 946 10.1093/glycob/cwl012 10.1093/glycob/cwl012 1:CAS:528:DC%2BD28Xps1ygs70%3D (Pubitemid 44390935)
3. EJM Van Damme WJ Peumans A Barre P Rougé 1998 Plant lectins: a composite of several distinct families of structurally and evolutionary related proteins with diverse biological roles Critical Reviews in Plant Sciences 17 575 692 10.1016/S0735-2689(98)00365-7 10.1016/S0735-2689(98)00365-7 (Pubitemid 29000295)
4. H Rüdiger HJ Gabios 2001 Plant lectins: occurrence, biochemistry, functions and applications Glycoconjugate Journal 18 589 613 10.1023/A:1020687518999 10.1023/A:1020687518999 (Pubitemid 35341855)
5. RA Moreira CC Castelo-Branco ACO Monteiro RO Tavares LM Beltramini 1998 Isolation and partial characterization of a lectin from Artocarpus incisa L. seeds Phytochemistry 47 1183 1188 10.1016/S0031-9422(97)00753-X 10.1016/S0031-9422(97)00753-X 1:CAS:528:DyaK1cXjsV2htbc%3D (Pubitemid 28232495)
6. N Pineau JL Pousset JL Preud'Homme P Aucouturier 1990 Structural and functional similarities of breadfruit seed lectin and jacalin Molecular Immunology 27 237 240 10.1016/0161-5890(90)90135-M 10.1016/0161-5890(90)90135-M 1:CAS:528:DyaK3cXktlyltLw%3D (Pubitemid 20111693)
7. PT Campana DI Moraes ACO Monteiro-Moreira LM Beltramini 2002 Unfolding and refolding studies of frutalin, a tetrameric D-galactose binding lectin European Journal of Biochemistry 269 753 758 10.1046/j.0014-2956.2002.02742.x 10.1046/j.0014-2956.2002.02742.x 1:CAS:528:DC%2BD38XhtlKntb0%3D (Pubitemid 34176815)
8. AC Brando-Lima RF Saldanha-Gama MGMO Henriques ACO Monteiro-Moreira RA Moreira C Barja-Fidalgo 2005 Frutalin, a galactose-binding lectin, induces chemotaxis and rearrangement of actin cytoskeleton in human neutrophils: Involvement of tyrosine kinase and phosphoinositide 3-kinase Toxicology and Applied Pharmacology 208 145 154 10.1016/j.taap.2005.02.012 10.1016/j.taap.2005. 02.012 1:CAS:528:DC%2BD2MXhtVKjs7zP (Pubitemid 41377130)
9. AC Brando-Lima RF Saldanha-Gama CR Pereira CG Villela ALF Sampaio ACO Monteiro-Moreira MGMO Henriques RA Moreira C Barja-Fidalgo 2006 Involvement of phosphatidylinositol-3 kinase-Akt and nuclear factor kappa-B pathways in the effect of frutalin on human lymphocyte International Immunopharmacology 6 465 472 10.1016/j.intimp.2005.09.008 10.1016/j.intimp.2005.09.008 1:CAS:528:DC%2BD28Xls1WmtA%3D%3D (Pubitemid 43120461)
10. C Oliveira W Felix RA Moreira JA Teixeira L Domingues 2008 Expression of frutalin, an α-D-galactose-binding jacalin-related lectin, in the yeast Pichia pastoris Protein Expression and Purification 60 188 193 10.1016/j.pep.2008.04.008 10.1016/j.pep.2008.04.008 1:CAS:528: DC%2BD1cXns1ahsb0%3D
11. AA Sahasrabuddhe SM Gaikwad MV Krishnasastry MI Khan 2004 Studies on recombinant single chain jacalin lectin reveal reduced affinity for saccharides despite normal folding like native Jacalin Protein Science 13 3264 3273 10.1110/ps.04968804 10.1110/ps.04968804 1:CAS:528:DC%2BD2cXhtVCjsLvK (Pubitemid 39557796)
12. S Luo D Zhangsun K Tang 2005 Functional GNA expressed in Escherichia coli with high efficiency and its effect on Ceratovacuna lanigera Zehntner Applied Microbiology and Biotechnology 69 184 191 10.1007/s00253-005-0042-6 10.1007/s00253-005-0042-6 1:CAS:528:DC%2BD2MXhtlWktrfL (Pubitemid 41795092)
13. J-F Jiang Y Han L-J Xing Y-Y Xu Z-H Xu K Chong 2006 Cloning and expression of a novel cDNA encoding a mannose-specific jacalin-related lectin from Oryza sativa Toxicon 47 133 139 10.1016/j.toxicon.2005.10.010 10.1016/j.toxicon.2005.10.010 1:CAS:528:DC%2BD28Xht1OisQ%3D%3D (Pubitemid 43042404)
14. L Lin J Lu H Zeng Z Liang Y Zhou J Lin X Sun K Tang 2008 Molecular cloning and characterization of a mannose-binding lectin gene from Pinellia cordata Molecular Biology Reports 35 641 647 10.1007/s11033-007-9134-y 10.1007/s11033-007-9134-y 1:CAS:528:DC%2BD1cXhsVKjtrrO
15. C Houlès Astoul WJ Peumans EJM Van Damme A Barre Y Bourne P Rougé 2002 The size, shape and specificity of the sugar-binding site of the jacalin-related lectins is profoundly affected by the proteolytic cleavage of the subunits The Biochemical Journal 367 817 824 10.1042/BJ20020856 10.1042/BJ20020856 (Pubitemid 35305721)
16. H Yang TH Czapla 1993 Isolation and characterization of cDNA clones encoding jacalin isolectins The Journal of Biological Chemistry 268 5905 5910 1:CAS:528:DyaK3sXksVemu7Y%3D
17. E Ruffet N Paquet S Frutiger GJ Hughes J-C Jaton 1992 Structural and electron-microscopic studies of jacalin from jackfruit (Artocarpus integrifolia) show that this lectin is a 65 kDa tetramer The Biochemical Journal 286 131 134 1:CAS:528:DyaK3sXisV2qu7o%3D
18. NM Young RAZ Johnston DC Watson 1991 The amino acid sequences of jacalin and the Maclura pomifera agglutinin FEBS Letters 282 382 384 10.1016/0014-5793(91)80518-8 10.1016/0014-5793(91)80518-8 1:CAS:528: DyaK38XltlyrtQ%3D%3D
19. SK Mahanta S Sanker NV Rao MJ Swamy A Surolia 1992 Primary structure of a Thomsen-Friedenreich-antigen-specific lectin, jacalin [Artocarpus integrifolia (jack fruit) agglutinin]. Evidence for the presence of an internal repeat The Biochemical Journal 284 95 101 1:CAS:528:DyaK3sXhvVKjur0%3D
20. EJM Van Damme B Hause J Hu A Barre P Rougé P Proost WJ Peumans 2002 Two distinct jacalin-related lectins with a different specificity and subcellular location are major vegetative storage proteins in the bark of the black mulberry tree Plant Physiology 130 757 769 10.1104/pp.005892 10.1104/pp.005892 (Pubitemid 35224780)
21. M Abdul Rahman S Anuar Karsani I Othman P Shafinaz Abdul Rahman O Haji Hashim 2002 Galactose-binding lectin from the seeds of champedak (Artocarpus integer): sequences of its subunits and interactions with human serum O-glycosylated glycoproteins Biochemical and Biophysical Research Communications 295 1007 1013 10.1016/S0006-291X(02)00795-7 10.1016/S0006-291X(02)00795-7 1:CAS:528:DC%2BD38XltlKgt7g%3D (Pubitemid 35041686)
22. LD Ngoc M Brillard J Hoebeke 1993 The α and β-subunits of jacalins are cleavage products from a 17-kDa precursor Biochimica et Biophysica Acta 1156 219 222 1:CAS:528:DyaK3sXktVegtLs%3D
23. LLP daSilva JB Molfetta-Machado A Panunto-Castelo J Denecke GH Goldman M-C Roque-Barreira MHS Goldman 2005 cDNA cloning and functional expression of KM+, the mannose-binding lectin from Artocarpus integrifolia seeds Biochimica et Biophysica Acta 1726 251 260 1:CAS:528:DC%2BD2MXht1Gns77J
24. AA Sahasrabuddhe N Ahmed MV Krishnasastry 2006 Stress-induced phosphorylation of caveolin-1 and p38, and down-regulation of EGFr and ERK by the dietary lectin jacalin in two human carcinoma cell lines Cell Stress & Chaperones 11 135 147 10.1379/CSC-160R.1 10.1379/CSC-160R.1 1:CAS:528: DC%2BD28XntF2htbo%3D (Pubitemid 43948353)
25. J Yin G Li X Ren G Herrler 2007 Select what you need: A comparative evaluation of the advantages and limitations of frequently used expression systems for foreign genes Journal of Biotechnology 127 335 347 10.1016/j.jbiotec.2006.07.012 10.1016/j.jbiotec.2006.07.012 1:CAS:528: DC%2BD28Xht1Kmu7fF (Pubitemid 44827384)
26. JW Tobias T-E Shrader G Rocap A Varshavsky 1991 The N-end rule in bacteria Science 254 1374 1377 10.1126/science.1962196 10.1126/science.1962196 1:CAS:528:DyaK38XjslOjsw%3D%3D
27. P-H Hirel J-M Schmitter P Dessen G Fayat S Blanquet 1989 Extent of N-terminal methionine excision from Escherichia coli proteins is governed by the side-chain length of the penultimate amino acid Proceedings of the National Academy of Sciences of the United States of America 86 8247 8251 10.1073/pnas.86.21.8247 10.1073/pnas.86.21.8247 1:CAS:528:DyaK3cXktFyh
28. T Lundstedt E Seifert L Abramo B Thelin A Nyström J Pettersen R Bergman 1998 Experimental design and optimization Chemometrics and Intelligent Laboratory Systems 42 3 40 10.1016/S0169-7439(98)00065-3 10.1016/S0169-7439(98) 00065-3 1:CAS:528:DyaK1cXls1OksLs%3D (Pubitemid 28440359)