HaloTag7: A genetically engineered tag that enhances bacterial expression of soluble proteins and improves protein purification

Protein Expression and Purification

Volumn 68, Issue 1, 2009, Pages 110-120

  Ohana, Rachel Friedman ^a   Encell, Lance P ^a   Zhao, Kate ^a   Simpson, Dan ^a   Slater, Michael R ^a   Urh, Marjeta ^a   Wood, Keith V ^a  

^a PROMEGA CORPORATION   (United States)

Author keywords

Enhanced expression in E. coli; Fusion tag; HaloTag; HaloTag7; Protein expression; Protein labeling; Protein purification; Protein solubility

Indexed keywords

HYBRID PROTEIN;

ARTICLE; CHEMISTRY; ESCHERICHIA COLI; GEL CHROMATOGRAPHY; GENETICS; HUMAN; METABOLISM; METHODOLOGY; PILOT STUDY; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN ENGINEERING; SOLUBILITY;

CHROMATOGRAPHY, GEL; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; HUMANS; PILOT PROJECTS; PROTEIN ENGINEERING; RECOMBINANT FUSION PROTEINS; SOLUBILITY;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; TOBACCO ETCH VIRUS;

EID: 68349127373     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2009.05.010     Document Type: Article

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