Recombinant enterokinase light chain with affinity tag: Expression from Saccharomyces cerevisiae and its utilities in fusion protein technology

Biotechnology and Bioengineering

Volumn 75, Issue 6, 2001, Pages 718-724

  Choi, Seong Il ^a   Song, Hye Won ^b   Moon, Jae Woong ^c   Seong, Baik L ^a  

^a Yonsei University   (South Korea)

^b Protheon Incorporated   (South Korea)

^c Hanhyo Institute of Technology   (South Korea)

Author keywords

Bovine enterokinase light chain; Cleavage; Fusion protein; Histidine tag

Indexed keywords

BIODEGRADATION; CHROMATOGRAPHY; FERMENTATION; PROTEINS;

CULTURE MEDIUM;

BIOTECHNOLOGY;

ENTEROPEPTIDASE; HISTIDINE; HYBRID PROTEIN;

AMINO TERMINAL SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; CONTAMINATION; DISULFIDE BOND; ENZYME ACTIVITY; FERMENTATION; HYDROLYSIS; MOLECULAR RECOGNITION; NONHUMAN; PROTEIN DEGRADATION; PROTEIN METABOLISM; SACCHAROMYCES CEREVISIAE;

AFFINITY LABELS; BASE SEQUENCE; CHROMATOGRAPHY, AFFINITY; DNA PRIMERS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENTEROPEPTIDASE; HYDROLYSIS; RECOMBINANT FUSION PROTEINS; SACCHAROMYCES CEREVISIAE;

BOVINAE; SACCHAROMYCES CEREVISIAE;

EID: 0035924096     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.10082     Document Type: Article

References (20)

1. Production of recombinant bovine enterokinase catalytic subunit Escherichia coli using the novel secretory fusion partner DsbA
2. Expression of atrial natriuretic factor as a cleavable fusion with chloramphenicol acetyltransferase in Escherichia coli
3. Crystal structure of bovine trypsinogen at 1.8 A resolution
4. The purification and characterization of bovine enterokinase from membrane fragments in the duodenal musosal fluid
5. Hydrolysis of artificial substrate by enterokinase and trypsin and the development of a sensitive specific assay for enterokinase in serum
6. Enterokinase, the initiator of intestinal digestion, is a mosaic protease composed of a distinctive assortment of domains
7. Formation of trypsin from crystalline trypsinogen by means of enterokinase
8. Clonging and functional expression of a cDNA encoding the catalytic subunit of bovine enterokinase
9. Novel secretion system of recombinant Saccharomyces cerevisiae using an N-terminus residue of human IL-1β as secretion enhancer
10. Specificity of bovine enterokinase toward protein substrates
11. The preparation and properties of the catalytic subunit of bovine enterokinase
12. Crystal structure of enteropeptidase light chain complexed with an analog of the trypsinogen activation peptide
13. Purification and specificity of porcine enterokinase
14. Metal chelate affinity chromatography, a new approach to protein fractionation
15. Immobilized metal ion affinity chromatography
16. DNA sequencing with chain-terminating inhibitors
17. High-level production of human growth hormone in Escherichia coll by a simple recombinant process
18. Production of a recombinant bovine enterokinase catalytic subunit in the methylotropic yeast pichia pastoris
19. The action of enterokinase on trypsingogen
20. Activation of recombinant proenteropeptidase by duodenase