Escherichia coli maltose-binding protein as a molecular chaperone for recombinant intracellular cytoplasmic single-chain antibodies

Journal of Molecular Biology

Volumn 312, Issue 1, 2001, Pages 79-93

  Bach, Horacio ^a   Mazor, Yariv ^a   Shaky, Shelly ^a   Shoham Lev, Atar ^a   Berdichevsky, Yevgeny ^a   Gutnick, David L ^a   Benhar, Itai ^a  

^a TEL AVIV UNIVERSITY   (Israel)

Author keywords

Cytoplasmic intrabodies; Fusion protein; Maltose binding protein; Molecular chaperone; ScFv fragments

Indexed keywords

CHAPERONE; DNA FRAGMENT; HYBRID PROTEIN; IMMUNOGLOBULIN FRAGMENT; MALTOSE BINDING PROTEIN; MBP SCFV FUSION PROTEIN; RECOMBINANT ANTIBODY; RECOMBINANT SINGLE CHAIN ANTIBODY; UNCLASSIFIED DRUG;

ANIMAL CELL; ANTIBODY PRODUCING CELL; ARTICLE; BACTERIOPHAGE; BIOTECHNOLOGY; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; CYTOPLASM; DISULFIDE BOND; DNA LIBRARY; ESCHERICHIA COLI; GENE FUSION; HUMAN; MOLECULAR STABILITY; MOUSE; NONHUMAN; PHAGE DISPLAY; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN FOLDING; SOLUBILITY;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; MURINAE;

EID: 0035823143     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.2001.4914     Document Type: Article

References (83)

1. Single-chain antigen-binding proteins
2. Protein engineering of antibody binding sites: Recovery of specific activity in an anti-digoxin single-chain Fv analogue produced in Escherichia coli
3. A new means of inducibly inactivating a cellular protein
4. Intracellular immunization with cytosolic recombinant antibodies
5. Intracellular antibodies (intrabodies) as research reagents and therapeutic molecules for gene therapy
6. Intrabodies: Turning the humoral immune system outside in for intracellular immunization
7. Expression of an antibody fragment at high levels in the bacterial cytoplasm
8. In vitro folding and thermodynamic stability of an antibody fragment selected in vivo for high expression levels in Escherichia coli cytoplasm
9. Targeting vectors for intracellular immunisation
10. An integrated vector system for the eukaryotic expression of antibodies or their fragments after selection from phage display libraries
11. The selection of intracellular antibodies
12. Molecular and cellular aspects of thiol-disulfide exchange
13. The immunoglobulin superfamily-domains for cell surface recognition
14. A natural antibody missing a cysteine in VH: Consequences for thermodynamic stability and folding
15. Redox state of single chain Fv fragments targeted to the endoplasmic reticulum, cytosol and mitochondria
16. A soluble immunoglobulin variable domain without a disulfide bridge: Construction, accumulation in the cytoplasm of E. coli, purification and physicochemical characterization
17. Contribution of the intramolecular disulfide bridge to the folding stability of REIv, the variable domain of a human immunoglobulin kappa light chain
18. Stability engineering of antibody single-chain Fv fragments
19. Molecular and virological effects of intracellular anti-Rev single-chain variable fragments on the expression of various human immunodeficiency virus-1 strains
20. Inhibition of HIV-1 Tat-mediated LTR transactivation and HIV-1 infection by anti-Tat single chain intrabodies
21. Inhibition of murine leukemia virus retrotranscription by the intracellular expression of a phage-derived antireverse transcriptase antibody fragment
22. A single-chain antibody fragment is functionally expressed in the cytoplasm of both Escherichia coli and transgenic plants
23. High level accumulation of single-chain variable fragments in the cytosol of transgenic Petunia hybrida
24. Extended half-life and elevated steady-state level of a single-chain Fv intrabody are critical for specific intracellular retargeting of its antigen, caspase-7
25. Correlation between in vitro stability and in vivo performance of anti-GCN4 intrabodies as cytoplasmic inhibitors
26. Intrabody construction and expression III: Engineering hyperstable V(H) domains
27. Evolutionary approaches to protein engineering
28. Fusion proteins in biotechnology
29. Engineering proteins to facilitate bioprocessing
30. Gene fusion expression systems in Escherichia coli
31. Characterization of a new intrabody directed against the N-terminal region of human p53
32. Glutathione S-transferase can be used as a C-terminal, enzymatically active dimerization module for a recombinant protease inhibitor, and functionally secreted into the periplasm of Escherichia coli
33. A thioredoxin gene fusion expression system that circumvents inclusion body formation in the E. coli cytoplasm
34. Vectors that facilitate the expression and purification of foreign peptides in Escherichia coli by fusion to maltose-binding protein
35. Escherichia coli maltose-binding protein is uncommonly effective at promoting the solubility of polypeptides to which it is fused
36. Expression and purification of the first nucleotide-binding domain and linker region of human multidrug resistance gene product: Comparison of fusions to glutathione S-transferase, thioredoxin and maltose-binding protein
37. Expression of a Chlamydia anticarbohydrate single-chain antibody as a maltose binding fusion protein
38. Bifunctional hybrids between the variable domains of an immunoglobulin and the maltose-binding protein of Escherichia coli: Production, purification and antigen binding
39. Production of a soluble and active MBP-scFv fusion: Favorable effect of the leaky tolR strain
40. Improved expression characteristics of single-chain Fv fragments when fused downstream of the Escherichia coli maltose-binding protein or upstream of a single immunoglobulin-constant domain
41. Purification and activity of a wheat 9-kDa lipid transfer protein expressed in Escherichia coli as a fusion with the maltose binding protein
42. High-level expression of soluble protein in Escherichia coli using a His6-tag and maltose-binding-protein double-affinity fusion system
43. Expression of a recombinant Toxoplasma gondii ROP2 fragment as a fusion protein in bacteria circumvents insolubility and proteolytic degradation
44. Expression of recombinant Norwalk-like virus capsid proteins using a bacterial system and the development of its immunologic detection
45. Expression and purification of recombinant proteins by fusion to maltose-binding protein
46. Autoprocessing of the HIV-1 protease using purified wild-type and mutated fusion proteins expressed at high levels in Escherichia coli
47. Activity of purified NIFA, a transcriptional activator of nitrogen fixation genes
48. Rat liver mitochondrial processing peptidase. Both alpha- and beta-subunits are required for activity
49. Intra- and extra-cellular expression of an scFv antibody fragment in E. coli: Effect of bacterial strains and pathway engineering using GroES/L chaperonins
50. Highly efficient selection of phage antibodies mediated by display of antigen as Lpp-OmpA' fusions on live bacteria
51. Targeted therapy of schwannoma cells in immunocompetent rats with an erbB2-specific antibody-toxin
52. Improving in vivo folding and stability of a single-chain Fv antibody fragment by loop grafting
53. Disrupting the hydrophobic patches at the antibody variable/constant domain interface: Improved in vivo folding and physical characterization of an engineered scFv fragment
54. Mutations that allow disulfide bond formation in the cytoplasm of Escherichia coli
55. Functional expression of a single-chain anti-progesterone antibody fragment in the cytoplasm of a mutant Escherichia coli
56. Functional antibody single-chain fragments from the cytoplasm of Escherichia coli: Influence of thioredoxin reductase (TrxB)
57. Phage display of a cellulose binding domain from Clostridium thermocellum and its application as a tool for antibody engineering
58. Characterization of purified recombinant Bet v 1 with authentic N terminus, cloned in fusion with maltose-binding protein
59. Expression and biological activity of genetic fusions between MalE, the maltose binding protein from Escherichia coli and portions of CD4, the T-cell receptor of the AIDS virus
60. Recognition of E. coli tryptophan synthase by single-chain Fv fragments: Comparison of PCR-cloning variants with the parental antibodies
61. Energetic and kinetic contributions of contact residues of antibody D1.3 in the interaction with lysozyme
62. Selection of phage antibodies to surface epitopes of Phytophthora infestans
63. An intrinsically stable antibody scFv fragment can tolerate the loss of both disulfide bonds and fold correctly
64. Antibody-mediated activation of a defective beta-D-galactosidase: Dimeric form of the activatable mutant enzyme
65. Molecular chaperones: Avoiding the crowd
66. Molecular chaperones: Pathways and networks
67. Folding of newly translated proteins in vivo: The role of molecular chaperones
68. Chaperone properties of the bacterial periplasmic substrate-binding proteins
69. Single amino acid substitutions on the surface of Escherichia coli maltose-binding protein can have a profound impact on the solubility of fusion proteins
70. Chaperone-assisted protein folding
71. How chaperones fold proteins
72. Maltose/maltodextrin system of Escherichia coli: Transport, metabolism, and regulation
73. Crystal structures and solution conformations of a dominant-negative mutant of Escherichia coli maltose-binding protein
74. Molecular chaperones: Proteins essential for the biogenesis of some macromolecular structures
75. Molecular chaperone functions of heat-shock proteins
76. The role of molecular chaperones in protein folding
77. High level Escherichia coli expression and production of a bivalent humanized antibody fragment
78. Matrix-assisted refolding of single-chain Fv-cellulose binding domain fusion proteins
79. Antibody mediated activation of a defective β-galactosidase (AMEF). Characterization of binding and activation processes
80. Immunoaffinity purification of FLAG epitope-tagged bacterial alkaline phosphatase using a novel monoclonal antibody and peptide elution
81. Construction, bacterial expression and characterization of a bifunctional single-chain antibody-phosphatase fusion protein targeted to the human erbB-2 receptor