New fusion protein systems designed to give soluble expression in Escherichia coli

Biotechnology and Bioengineering

Volumn 65, Issue 4, 1999, Pages 382-388

  Davis, Gregory D ^a,b   Elisee, Claude ^a   Mewham, Denton M ^a   Harrison, Roger G ^a  

^a University of Oklahoma   (United States)

^b CLONTECH Laboratories Inc   (United States)

Author keywords

Escherichia coil; Fusion protein; Purification; Soluble protein expression

Indexed keywords

AFFINITY CHROMATOGRAPHY; ESCHERICHIA COLI; HORMONES; MATHEMATICAL MODELS; PROBABILITY; PURIFICATION; SOLUBILITY; STATISTICS;

ANION EXCHANGE CHROMATOGRAPHY; FUSION PROTEINS; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY;

PROTEINS;

BACTERIAL PROTEIN; BLOOD CLOTTING FACTOR 10A; GAMMA INTERFERON; GROWTH HORMONE; HYBRID PROTEIN; INTERLEUKIN 3; PROTEINASE; RECOMBINANT PROTEIN; THIOREDOXIN;

AFFINITY CHROMATOGRAPHY; AMINO TERMINAL SEQUENCE; ANION EXCHANGE CHROMATOGRAPHY; ARTICLE; CYTOPLASM; ESCHERICHIA COLI; NONHUMAN; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN IMMOBILIZATION; SOLUBILITY; STATISTICAL MODEL;

ANIMALS; BACTERIAL PROTEINS; CATTLE; CYTOCHROME B GROUP; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; FERRITINS; GROWTH HORMONE; HEAT-SHOCK PROTEINS; HUMANS; INTERFERON TYPE II; INTERLEUKIN-3; PEPTIDE ELONGATION FACTORS; PROTEIN ENGINEERING; RECOMBINANT FUSION PROTEINS; THIOREDOXIN; TRANSCRIPTION FACTORS;

BACTERIA (MICROORGANISMS); BOVINAE; ESCHERICHIA; ESCHERICHIA COLI; NEGIBACTERIA;

EID: 0033589826     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0290(19991120)65:4<382::AID-BIT2>3.0.CO;2-I     Document Type: Article

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