A rapid and universal tandem-purification strategy for recombinant proteins

Protein Science

Volumn 16, Issue 12, 2007, Pages 2726-2732

  Mccluskey, Andrew J ^a,b   Poon, Gregory M K ^b   Gariépy, Jean ^a,b,c  

^a UNIVERSITY OF TORONTO   (Canada)

^b PRINCESS MARGARET HOSPITAL   (Canada)

^c UNIVERSITY OF TORONTO   (Canada)

Author keywords

Hydrophobic interaction chromatography; Immobilized metal affinity chromatography; Protein purification; Tandem affinity purification

Indexed keywords

CALMODULIN; ENHANCED GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; POLYHISTIDINE TAG; PROTEIN P53; RECOMBINANT PROTEIN; THROMBIN;

AMINO TERMINAL SEQUENCE; ARTICLE; CELL LYSATE; CHROMATOGRAPHY; ESCHERICHIA COLI; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; LARGE SCALE PRODUCTION; METHODOLOGY; MOLECULAR RECOGNITION; PRIORITY JOURNAL; PROTEIN ENGINEERING; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN SYNTHESIS; RECOMBINANT DNA TECHNOLOGY;

AMINO ACID SEQUENCE; BASE SEQUENCE; CALMODULIN; CHROMATOGRAPHY, AFFINITY; GREEN FLUORESCENT PROTEINS; HUMANS; HYDROPHOBICITY; MOLECULAR SEQUENCE DATA; RECOMBINANT FUSION PROTEINS; TUMOR SUPPRESSOR PROTEIN P53;

ESCHERICHIA COLI;

EID: 36448981741     PISSN: 09618368     EISSN: 1469896X     Source Type: Journal    
DOI: 10.1110/ps.072894407     Document Type: Article

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