Recombinant expression and purification of the antimicrobial peptide magainin-2

Biotechnology Progress

Volumn 29, Issue 1, 2013, Pages 17-22

  Ramos, Reinaldo ^a   Moreira, Susana ^a   Rodrigues, Ana ^a   Gama, Miguel ^a   Domingues, Lucília ^a  

^a UNIVERSITY OF MINHO   (Portugal)

Author keywords

Antimicrobial peptide; Magainin 2; Recombinant protein

Indexed keywords

AFRICAN CLAWED FROG; ANTI-MICROBIAL ACTIVITY; ANTIMICROBIAL PEPTIDE; BIOLOGICALLY ACTIVE PEPTIDES; CARBOHYDRATE-BINDING MODULES; CHEMICAL CLEAVAGE; CLOSTRIDIUM THERMOCELLUM; FUSION PARTNERS; GRAM-NEGATIVE BACTERIA; LINKER SEQUENCE; MAGAININ-2; N-TERMINALS; RECOGNITION SITE; RECOMBINANT EXPRESSION; RECOMBINANT EXPRESSION SYSTEM; THERAPEUTIC APPLICATION; WIDE SPECTRUM;

BACTERIA; CARBOHYDRATES; CLONING; ESCHERICHIA COLI; FORMIC ACID; PEPTIDES; RECOMBINANT PROTEINS;

PURIFICATION;

MAGAININ 2 PEPTIDE, XENOPUS; MAGAININ DERIVATIVE; RECOMBINANT PROTEIN; XENOPUS PROTEIN;

ANIMAL; ARTICLE; ESCHERICHIA COLI; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; XENOPUS LAEVIS;

ANIMALS; ESCHERICHIA COLI; MAGAININS; RECOMBINANT PROTEINS; XENOPUS LAEVIS; XENOPUS PROTEINS;

CLOSTRIDIUM THERMOCELLUM; ESCHERICHIA COLI; FUNGI; NEGIBACTERIA; POSIBACTERIA; PROTOZOA; XENOPUS LAEVIS;

EID: 84873301395     PISSN: 87567938     EISSN: 15206033     Source Type: Journal    
DOI: 10.1002/btpr.1650     Document Type: Article

References (37)

1. Rossi LM, Rangasamy P, Zhang J, Qiu XQ, Wu GY. Research advances in the development of peptide antibiotics. J Pharm Sci. 2008; 97: 1060-1070.
2. Ajesh K, Sreejith K. Peptide antibiotics: an alternative and effective antimicrobial strategy to circumvent fungal infections. Peptides. 2009; 30: 999-1006.
3. Murakami T, Niwa M, Tokunaga F, Miyata T, Iwanaga S. Direct virus inactivation of tachyplesin I and its isopeptides from horseshoe crab hemocytes. Chemotherapy. 1991; 37: 327-334.
4. Morimoto M, Mori H, Otake T, Ueba N, Kunita N, Niwa M, Murakami T, Iwanaga S. Inhibitory effect of tachyplesin I on the proliferation of human immunodeficiency virus in vitro. Chemotherapy. 1991; 37: 206-211.
5. Hoskin DW, Ramamoorthy A. Studies on anticancer activities of antimicrobial peptides. Biochim Biophys Acta. 2008; 1778: 357-375.
6. Powers JP, Hancock RE. The relationship between peptide structure and antibacterial activity. Peptides. 2003; 24: 1681-1691.
7. Hancock RE, Diamond G. The role of cationic antimicrobial peptides in innate host defences. Trends Microbiol. 2000; 8: 402-410.
8. Zasloff M. Magainins, a class of antimicrobial peptides from Xenopus skin: isolation, characterization of two active forms, and partial cDNA sequence of a precursor. Proc Natl Acad Sci U S A. 1987; 84: 5449-5453.
9. King JD, Mechkarska M, Coquet L, Leprince J, Jouenne T, Vaudry H, Takada K, Conlon JM. Host-defense peptides from skin secretions of the tetraploid frogs Xenopus petersii and Xenopus pygmaeus, and the octoploid frog Xenopus lenduensis (Pipidae). Peptides. 2012; 33: 35-43.
10. Westerhoff HV, Juretic D, Hendler RW, Zasloff M. Magainins and the disruption of membrane-linked free-energy transduction. Proc Natl Acad Sci U S A. 1989; 86: 6597-6601.
11. Cruciani RA, Barker JL, Zasloff M, Chen HC, Colamonici O. Antibiotic magainins exert cytolytic activity against transformed cell lines through channel formation. Proc Natl Acad Sci U S A. 1991; 88: 3792-3796.
12. Gallucci E, Meleleo D, Micelli S, Picciarelli V. Magainin 2 channel formation in planar lipid membranes: the role of lipid polar groups and ergosterol. Eur Biophys J. 2003; 32: 22-32.
13. Cruciani RA, Barker JL, Durell SR, Raghunathan G, Guy HR, Zasloff M, Stanley EF. Magainin 2, a natural antibiotic from frog skin, forms ion channels in lipid bilayer membranes. Eur J Pharmacol. 1992; 226: 287-296.
14. Grant EJr, Beeler TJ, Taylor KM, Gable K, Roseman MA. Mechanism of magainin 2a induced permeabilization of phospholipid vesicles. Biochemistry. 1992; 31: 9912-9918.
15. Gilkes NR, Henrissat B, Kilburn DG, Miller RC Jr, Warren RA. Domains in microbial beta-1, 4-glycanases: sequence conservation, function, and enzyme families. Microbiol Rev. 1991; 55: 303-315.
16. Tormo J, Lamed R, Chirino AJ, Morag E, Bayer EA, Shoham Y, Steitz TA. Crystal structure of a bacterial family-III cellulose-binding domain: a general mechanism for attachment to cellulose. EMBO J. 1996; 15: 5739-5751.
17. Guerreiro CI, Fontes CM, Gama M, Domingues L. Escherichia coli expression and purification of four antimicrobial peptides fused to a family 3 carbohydrate-binding module (CBM) from Clostridium thermocellum. Prot Expr Purif. 2008; 59: 161-168.
18. Wright O, Yoshimi T, Tunnacliffe A. Recombinant production of cathelicidin-derived antimicrobial peptides in Escherichia coli using an inducible autocleaving enzyme tag. N Biotechnol. 2012; 29: 352-358.
19. Zorko M, Jerala R. Production of recombinant antimicrobial peptides in bacteria. Methods Mol Biol. 2010; 618: 61-76.
20. Li Y. Recombinant production of antimicrobial peptides in Escherichia coli: a review. Prot Expr Purif. 2011; 80: 260-267.
21. Barrell PJ, Liew OW, Conner AJ. Expressing an antibacterial protein in bacteria for raising antibodies. Prot Expr Purif. 2004; 33: 153-159.
22. Waddell WJ. A simple ultraviolet spectrophotometric method for the determination of protein. J Lab Clin Med. 1956; 48: 311-314.
23. Vogt TC, Bechinger B. The interactions of histidine-containing amphipathic helical peptide antibiotics with lipid bilayers. The effects of charges and pH. J Biol Chem. 1999; 274: 29115-29121.
24. Ingham AB, Moore RJ. Recombinant production of antimicrobial peptides in heterologous microbial systems. Biotechnol Appl Biochem. 2007; 47: 1-9.
25. Garcia-Fruitos E. Inclusion bodies: a new concept. Microb Cell Fact. 2010; 9: 80.
26. Rodriguez-Carmona E, Cano-Garrido O, Seras-Franzoso J, Villaverde A, Garcia-Fruitos E. Isolation of cell-free bacterial inclusion bodies. Microb Cell Fact. 2010; 9: 71.
27. Ramos R, Domingues L, Gama M. Escherichia coli expression and purification of LL37 fused to a family III carbohydrate-binding module from Clostridium thermocellum. Prot Expr Purif. 2010; 71: 1-7.
28. Ramos R, Silva JP, Rodrigues AC, Costa R, Guardao L, Schmitt F, Soares R, Vilanova M, Domingues L, Gama M. Wound healing activity of the human antimicrobial peptide LL37. Peptides. 2011; 32: 1469-1476.
29. Tomme P, Boraston A, McLean B, Kormos J, Creagh AL, Sturch K, Gilkes NR, Haynes CA, Warren RA, Kilburn DG. Characterization and affinity applications of cellulose-binding domains. J Chromatogr B Biomed Sci Appl. 1998; 715: 283-296.
30. Giglione C, Boularot A, Meinnel T. Protein N-terminal methionine excision. Cell Mol Life Sci. 2004; 61: 1455-1474.
31. Sovadinova I, Palermo EF, Huang R, Thoma LM, Kuroda K. Mechanism of polymer-induced hemolysis: nanosized pore formation and osmotic lysis. Biomacromolecules. 2011; 12: 260-268.
32. Sovadinova I, Palermo EF, Urban M, Mpiga P, Caputo GA, Kuroda K. Activity and mechanism of antimicrobial peptide-mimetic amphiphilic polymethacrylate derivatives. Polymers. 2011; 3: 1512-1532.
33. Melo MN, Ferre R, Castanho MA. Antimicrobial peptides: linking partition, activity and high membrane-bound concentrations. Nat Rev Microbiol. 2009; 7: 245-250.
34. Dathe M, Nikolenko H, Meyer J, Beyermann M, Bienert M. Optimization of the antimicrobial activity of magainin peptides by modification of charge. FEBS Lett. 2001; 501: 146-150.
35. Hwang SW, Lee JH, Park HB, Pyo SH, So JE, Lee HS, Hong SS, Kim JH. A simple method for the purification of an antimicrobial peptide in recombinant Escherichia coli. Mol Biotechnol. 2001; 18: 193-198.
36. Lee JH, Kim JH, Hwang SW, Lee WJ, Yoon HK, Lee HS, Hong SS. High-level expression of antimicrobial peptide mediated by a fusion partner reinforcing formation of inclusion bodies. Biochem Biophys Res Commun. 2000; 277: 575-580.
37. Fan B, Li N. Design and synthesis of a Magainin2 fusion protein gene suitable for a mammalian expression system. Transgenic Res. 2009; 18: 99-112.