High throughput construction and small scale expression screening of multi-tag vectors in Escherichia coli

Methods

Volumn 55, Issue 1, 2011, Pages 29-37

  Bird, Louise E ^a,b  

^a UNIVERSITY OF OXFORD   (United Kingdom)

^b RUTHERFORD APPLETON LABORATORY   (United Kingdom)

Author keywords

Escherichia coli; Hexa histidine tag; InFusion cloning; N terminal fusion; Protein expression; Solubilising protein

Indexed keywords

HYBRID PROTEIN;

ARTICLE; ESCHERICHIA COLI; EXPRESSION VECTOR; GENE EXPRESSION; HIGH THROUGHPUT SCREENING; MOLECULAR CLONING; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN DEGRADATION; PROTEIN EXPRESSION;

ANIMALS; BASE SEQUENCE; CLONING, MOLECULAR; ESCHERICHIA COLI; GENE EXPRESSION; GENETIC VECTORS; HIGH-THROUGHPUT SCREENING ASSAYS; HISTIDINE; HUMANS; MICE; MOLECULAR BIOLOGY; MOLECULAR SEQUENCE DATA; NEISSERIA MENINGITIDIS; OLIGOPEPTIDES; PSEUDOMONAS AERUGINOSA; RECOMBINANT FUSION PROTEINS; SOLUBILITY;

ESCHERICHIA COLI;

EID: 80053636591     PISSN: 10462023     EISSN: 10959130     Source Type: Journal    
DOI: 10.1016/j.ymeth.2011.08.002     Document Type: Article

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