-
1
-
-
0028145744
-
A rapid one-step method to purify baculovirus-expressed human estrogen receptor to be used in the analysis of the oxytocin promoter
-
Beekman JM, Cooney AJ, Elliston JF, Tsai SY, Tsai MJ (1994) A rapid one-step method to purify baculovirus-expressed human estrogen receptor to be used in the analysis of the oxytocin promoter. Gene 146:285-289
-
(1994)
Gene
, vol.146
, pp. 285-289
-
-
Beekman, J.M.1
Cooney, A.J.2
Elliston, J.F.3
Tsai, S.Y.4
Tsai, M.J.5
-
2
-
-
0026680845
-
Interaction cloning: Identification of a helix-loop-helix zipper protein that interacts with c-fos
-
Blanar MA, Rutter WJ (1992) Interaction cloning: identification of a helix-loop-helix zipper protein that interacts with c-fos. Science 256:1014-1018
-
(1992)
Science
, vol.256
, pp. 1014-1018
-
-
Blanar, M.A.1
Rutter, W.J.2
-
3
-
-
0012381236
-
Identification of the calmodulin-binding domain of skeletal muscle myosin light chain kinase
-
Blumenthal DK, Takio K, Edelman A, Charbonneau H, Titani K, Walsh KA, Krebs EG (1985) Identification of the calmodulin-binding domain of skeletal muscle myosin light chain kinase. Proc Natl Acad Sci USA 82:3187-3191
-
(1985)
Proc Natl Acad Sci USA
, vol.82
, pp. 3187-3191
-
-
Blumenthal, D.K.1
Takio, K.2
Edelman, A.3
Charbonneau, H.4
Titani, K.5
Walsh, K.A.6
Krebs, E.G.7
-
4
-
-
0031587388
-
Expression and purification of His-tagged beta-1,4 galactosyltransferase in yeast and in COS cells
-
Borsing L, Berger EG, Malissard M (1997) Expression and purification of His-tagged beta-1,4 galactosyltransferase in yeast and in COS cells. Biochem Biophys Res Commun 240:586-590
-
(1997)
Biochem Biophys Res Commun
, vol.240
, pp. 586-590
-
-
Borsing, L.1
Berger, E.G.2
Malissard, M.3
-
5
-
-
0036006255
-
Differential effects of short affinity tags on the crystallization of Pyrococcus furiosus maltodextrin-binding protein
-
Bucher MH, Evdokimov AG, Waugh DS (2002) Differential effects of short affinity tags on the crystallization of Pyrococcus furiosus maltodextrin-binding protein. Biol Cryst 58:392-397
-
(2002)
Biol Cryst
, vol.58
, pp. 392-397
-
-
Bucher, M.H.1
Evdokimov, A.G.2
Waugh, D.S.3
-
6
-
-
0034965749
-
Intein-mediated rapid purification of Cre recombinase
-
Cantor EJ, Chong S (2001) Intein-mediated rapid purification of Cre recombinase. Protein Expr Purif 22:135-140
-
(2001)
Protein Expr Purif
, vol.22
, pp. 135-140
-
-
Cantor, E.J.1
Chong, S.2
-
7
-
-
0000167256
-
A viral cleavage site cassette: Identification of amino acid sequences required for tobacco etch virus polyprotein processing
-
Carrington JC, Dougherty WG (1988) A viral cleavage site cassette: identification of amino acid sequences required for tobacco etch virus polyprotein processing. Proc Natl Acad Sci USA 85:3391-3395
-
(1988)
Proc Natl Acad Sci USA
, vol.85
, pp. 3391-3395
-
-
Carrington, J.C.1
Dougherty, W.G.2
-
8
-
-
0033026779
-
2+-carboxymethylaspartate-agarose superflow, as demonstrated by one-step purification of lactate dehydrogenase from chicken breast
-
2+-carboxymethylaspartate-agarose superflow, as demonstrated by one-step purification of lactate dehydrogenase from chicken breast. Biotechnol Appl Biochem 29:19-24
-
(1999)
Biotechnol Appl Biochem
, vol.29
, pp. 19-24
-
-
Chaga, G.1
Hopp, J.2
Nelson, P.3
-
9
-
-
0032757296
-
Natural poly-histidine affinity tag for purification of recombinant proteins on cobalt(II)-carboxymethylaspartate crosslinked agarose
-
Chaga G, Bochkariov DE, Jokhadze GG, Hopp J, Nelson P (1999b) Natural poly-histidine affinity tag for purification of recombinant proteins on cobalt(II)-carboxymethylaspartate crosslinked agarose. J Chromatogr A 864:257-256
-
(1999)
J Chromatogr A
, vol.864
, pp. 257-256
-
-
Chaga, G.1
Bochkariov, D.E.2
Jokhadze, G.G.3
Hopp, J.4
Nelson, P.5
-
10
-
-
0022354535
-
Thrombin specificity. Requirement for apolar amino acids adjacent to the thrombin cleavage site of polypeptide substrate
-
Chang JY (1985) Thrombin specificity. Requirement for apolar amino acids adjacent to the thrombin cleavage site of polypeptide substrate. Eur J Biochem 151:217-224
-
(1985)
Eur J Biochem
, vol.151
, pp. 217-224
-
-
Chang, J.Y.1
-
11
-
-
0022394537
-
Thrombin specificity. Selective cleavage of antibody light chains at the joints of variable with joining regions and joining with constant regions
-
Chang JY, Alkan SS, Hilschmann N, Braun DG (1985) Thrombin specificity. Selective cleavage of antibody light chains at the joints of variable with joining regions and joining with constant regions. Eur J Biochem 151:225-230
-
(1985)
Eur J Biochem
, vol.151
, pp. 225-230
-
-
Chang, J.Y.1
Alkan, S.S.2
Hilschmann, N.3
Braun, D.G.4
-
12
-
-
0028267319
-
Expression vectors for affinity purification and radiolabeling of proteins using Escherichia coli as host
-
Chen BP, Hai T (1994) Expression vectors for affinity purification and radiolabeling of proteins using Escherichia coli as host. Gene 139:73-75
-
(1994)
Gene
, vol.139
, pp. 73-75
-
-
Chen, B.P.1
Hai, T.2
-
13
-
-
0035924096
-
Recombinant enterokinase light chain with affinity tag: Expression from Saccharomyces cerevisiae and its utilities in fusion protein technology
-
Choi SI, Song HW, Moon JW, Seong BL (2001) Recombinant enterokinase light chain with affinity tag: expression from Saccharomyces cerevisiae and its utilities in fusion protein technology. Biotechnol Bioeng 75:718-724
-
(2001)
Biotechnol Bioeng
, vol.75
, pp. 718-724
-
-
Choi, S.I.1
Song, H.W.2
Moon, J.W.3
Seong, B.L.4
-
14
-
-
0029834656
-
Protein splicing involving the Saccharomyces cerevisiae VMA intein
-
Chong S, Shao Y, Paulus H, Benner J, Perler FB, Xu M-Q (1996) Protein splicing involving the Saccharomyces cerevisiae VMA intein. J Biol Chem 271:22159-22168
-
(1996)
J Biol Chem
, vol.271
, pp. 22159-22168
-
-
Chong, S.1
Shao, Y.2
Paulus, H.3
Benner, J.4
Perler, F.B.5
Xu, M.-Q.6
-
15
-
-
17744415288
-
Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element
-
Chong S, Mersha FB, Comb DG, Scott ME, Landry D, Vence LM, Perler FB, Benner J, Kucera RB, Hirvonen CA, Pelletier JJ, Paulus H, Xu M-Q (1997) Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element. Gene 192:271-281
-
(1997)
Gene
, vol.192
, pp. 271-281
-
-
Chong, S.1
Mersha, F.B.2
Comb, D.G.3
Scott, M.E.4
Landry, D.5
Vence, L.M.6
Perler, F.B.7
Benner, J.8
Kucera, R.B.9
Hirvonen, C.A.10
Pelletier, J.J.11
Paulus, H.12
Xu, M.-Q.13
-
16
-
-
0025293251
-
Thermodynamics of protein-peptide interactions in the ribonuclease S system studied by titration calorimetry
-
Connelly PR, Varadarajan R, Sturtevant JM, Richards FM (1990) Thermodynamics of protein-peptide interactions in the ribonuclease S system studied by titration calorimetry. Biochemistry 29:6108-6114
-
(1990)
Biochemistry
, vol.29
, pp. 6108-6114
-
-
Connelly, P.R.1
Varadarajan, R.2
Sturtevant, J.M.3
Richards, F.M.4
-
17
-
-
0025293361
-
Biotination of proteins in vivo. A post-translational modification to label, purify, and study proteins
-
Cronan JE Jr (1990) Biotination of proteins in vivo. A post-translational modification to label, purify, and study proteins. J Biol Chem 265:10327-10333
-
(1990)
J Biol Chem
, vol.265
, pp. 10327-10333
-
-
Cronan J.E., Jr.1
-
18
-
-
0033589826
-
New fusion protein systems designed to give soluble expression in Escherichia coli
-
Davis GD, Elisee C, Newham DM, Harrison RG (1999) New fusion protein systems designed to give soluble expression in Escherichia coli. Biotechnol Bioeng 65:382-388
-
(1999)
Biotechnol Bioeng
, vol.65
, pp. 382-388
-
-
Davis, G.D.1
Elisee, C.2
Newham, D.M.3
Harrison, R.G.4
-
19
-
-
0023680201
-
Vectors that facilitate the expression and purification of foreign peptides in Escherichia coli by fusion to maltose-binding protein
-
Di Guan C, Li P, Riggs PD, Inouye H (1988) Vectors that facilitate the expression and purification of foreign peptides in Escherichia coli by fusion to maltose-binding protein. Gene 67:21-30
-
(1988)
Gene
, vol.67
, pp. 21-30
-
-
Di Guan, C.1
Li, P.2
Riggs, P.D.3
Inouye, H.4
-
20
-
-
0024004266
-
Biochemical and mutational analysis of a plant virus polyprotein cleavage site
-
Dougherty WG, Carrington JC, Cary SM, Parks TD (1988) Biochemical and mutational analysis of a plant virus polyprotein cleavage site. EMBO J 7:1281-1287
-
(1988)
EMBO J
, vol.7
, pp. 1281-1287
-
-
Dougherty, W.G.1
Carrington, J.C.2
Cary, S.M.3
Parks, T.D.4
-
21
-
-
0024730021
-
Characterization of the catalytic residues of the tobacco etch virus 49-kDa proteinase
-
Dougherty WG, Parks TD, Cary SM, Bazan JF, Fletterick RJ (1989) Characterization of the catalytic residues of the tobacco etch virus 49-kDa proteinase. Virology 172:302-310
-
(1989)
Virology
, vol.172
, pp. 302-310
-
-
Dougherty, W.G.1
Parks, T.D.2
Cary, S.M.3
Bazan, J.F.4
Fletterick, R.J.5
-
23
-
-
12244251071
-
Purification of viral protein from infected plant tissues using the Strep-tag
-
Drucker M, German-Retana S, Esperandieu P, LeGall O, Blanc S (2002) Purification of viral protein from infected plant tissues using the Strep-tag. Botechnol Intl June:16-18
-
(2002)
Botechnol Intl
, vol.JUNE
, pp. 16-18
-
-
Drucker, M.1
German-Retana, S.2
Esperandieu, P.3
LeGall, O.4
Blanc, S.5
-
24
-
-
0021252896
-
Sequence of male gene and of its product, the maltose-binding protein of Escherichia coli K12
-
Duplay P, Bedouelle H, Fowler A, Zabin I, Saurin W, Hofnung M (1988) Sequence of male gene and of its product, the maltose-binding protein of Escherichia coli K12. J Biol Chem 259:10606-10613
-
(1988)
J Biol Chem
, vol.259
, pp. 10606-10613
-
-
Duplay, P.1
Bedouelle, H.2
Fowler, A.3
Zabin, I.4
Saurin, W.5
Hofnung, M.6
-
25
-
-
85036711609
-
Kinetics and thermodynamical properties of the monoclonal antibody M1 directed against the FLAG peptide
-
Lublijana, Slovenia, November 5-8, 2000
-
Einhauer A, Jungbauer A (2000) Kinetics and thermodynamical properties of the monoclonal antibody M1 directed against the FLAG peptide. 20th International symposium on the separation of proteins, peptides, and polynucleotides (ISPPP). Lublijana, Slovenia, November 5-8, 2000
-
(2000)
20th International Symposium on the Separation of Proteins, Peptides, and Polynucleotides (ISPPP)
-
-
Einhauer, A.1
Jungbauer, A.2
-
26
-
-
0036447573
-
Expression and purification of homogenous proteins in Saccharomyces cerevisiae based on ubiquitin-FLAG fusion
-
Einhauer A, Schuster M, Wasserbauer E, Jungbauer A (2002) Expression and purification of homogenous proteins in Saccharomyces cerevisiae based on ubiquitin-FLAG fusion. Protein Expr Purif 24:497-504
-
(2002)
Protein Expr Purif
, vol.24
, pp. 497-504
-
-
Einhauer, A.1
Schuster, M.2
Wasserbauer, E.3
Jungbauer, A.4
-
27
-
-
0033938006
-
Expanding baculovirus surface display
-
Ernst WJ, Spenger A, Toellner L, Katinger H, Grabherr RM (2000) Expanding baculovirus surface display. Eur J Biochem 267:4033-4039
-
(2000)
Eur J Biochem
, vol.267
, pp. 4033-4039
-
-
Ernst, W.J.1
Spenger, A.2
Toellner, L.3
Katinger, H.4
Grabherr, R.M.5
-
28
-
-
0022340978
-
Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product
-
Evan GI, Lewis GK, Ramsay G, Bishop JM (1985) Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product. Mol Cell Biol 5:3610-3616
-
(1985)
Mol Cell Biol
, vol.5
, pp. 3610-3616
-
-
Evan, G.I.1
Lewis, G.K.2
Ramsay, G.3
Bishop, J.M.4
-
29
-
-
0035445926
-
Detection of in vivo protein interactions between Snf1-related kinase subunits with intron-tagged epitope-labeling in plant cells
-
Ferrando A, Koncz-Lalman Z, Farras R, Tiburcio A, Schell J, Koncz C (2001) Detection of in vivo protein interactions between Snf1-related kinase subunits with intron-tagged epitope-labeling in plant cells. Nucleic Acids Res 29:3685-3693
-
(2001)
Nucleic Acids Res
, vol.29
, pp. 3685-3693
-
-
Ferrando, A.1
Koncz-Lalman, Z.2
Farras, R.3
Tiburcio, A.4
Schell, J.5
Koncz, C.6
-
30
-
-
0036180998
-
Molecular characterization and transcriptional analysis of adhE2, the gene encoding the NADH-dependent aldehyde/alcohol dehydrogenase responsible for butanol production in alcohologenic cultures of Clostridium acetobutylicum ATCC 824
-
Fontaine L, Meynial-Salles I, Girbal L, Yang X, Croux C, Soucaille P (2002) Molecular characterization and transcriptional analysis of adhE2, the gene encoding the NADH-dependent aldehyde/alcohol dehydrogenase responsible for butanol production in alcohologenic cultures of Clostridium acetobutylicum ATCC 824. J Bacteriol 184:821-830
-
(2002)
J Bacteriol
, vol.184
, pp. 821-830
-
-
Fontaine, L.1
Meynial-Salles, I.2
Girbal, L.3
Yang, X.4
Croux, C.5
Soucaille, P.6
-
31
-
-
0027212498
-
Solubilization and purification of enzymatically active glutathione S-transferase (pGEX) fusion proteins
-
Frangioni JV, Neel BG (1993) Solubilization and purification of enzymatically active glutathione S-transferase (pGEX) fusion proteins. Anal Biochem 210:179-187
-
(1993)
Anal Biochem
, vol.210
, pp. 179-187
-
-
Frangioni, J.V.1
Neel, B.G.2
-
32
-
-
0030963019
-
Toward a functional analysis of the yeast genome exhausted two-hybrid screens
-
Fromont-Racine M, Rain J-C, Legrain R, Legrain P (1997) Toward a functional analysis of the yeast genome exhausted two-hybrid screens. Nat Genet 16:277-282
-
(1997)
Nat Genet
, vol.16
, pp. 277-282
-
-
Fromont-Racine, M.1
Rain, J.-C.2
Legrain, R.3
Legrain, P.4
-
33
-
-
0037050026
-
Functional organization of the yeast proteome by systematic analysis of protein complexes
-
Gavin AC, Bosche M, Krause R, Grandi P, Marzioch M, Bauer A, Schultz J, Rick JM, Michon AM, Cruciat CM, Remor M, Hofert C, Schelder M, Brajenovic M, Ruffner H, Merino A, Klein K, Hudak M, Dickson D, Rudi T, Gnau V, Bauch A, Bastuck S, Huhse B, Leutwein C, Heurtier MA, Copley RR, Edelmann A, Querfurth E, Rybin V, Drewes G, Raida M, Bouwmeester T, Bork P, Seraphin B, Kuster B, Neubauer G, Superti-Furga G (2002) Functional organization of the yeast proteome by systematic analysis of protein complexes. Nature 415:141-147
-
(2002)
Nature
, vol.415
, pp. 141-147
-
-
Gavin, A.C.1
Bosche, M.2
Krause, R.3
Grandi, P.4
Marzioch, M.5
Bauer, A.6
Schultz, J.7
Rick, J.M.8
Michon, A.M.9
Cruciat, C.M.10
Remor, M.11
Hofert, C.12
Schelder, M.13
Brajenovic, M.14
Ruffner, H.15
Merino, A.16
Klein, K.17
Hudak, M.18
Dickson, D.19
Rudi, T.20
Gnau, V.21
Bauch, A.22
Bastuck, S.23
Huhse, B.24
Leutwein, C.25
Heurtier, M.A.26
Copley, R.R.27
Edelmann, A.28
Querfurth, E.29
Rybin, V.30
Drewes, G.31
Raida, M.32
Bouwmeester, T.33
Bork, P.34
Seraphin, B.35
Kuster, B.36
Neubauer, G.37
Superti-Furga, G.38
more..
-
34
-
-
0025275245
-
Expression and purification of a truncated recombinant streptococcal protein G
-
Goward CR, Murphy JP, Atkinson T, Barstow DA (1990) Expression and purification of a truncated recombinant streptococcal protein G. Biochem J 267:171-177
-
(1990)
Biochem J
, vol.267
, pp. 171-177
-
-
Goward, C.R.1
Murphy, J.P.2
Atkinson, T.3
Barstow, D.A.4
-
35
-
-
85036688750
-
Isolierung der trimeren hydrogenase aus Wolinella succinogenes durch streptactin-affinitätschromatographie
-
Groß R, Pisa R, Simon J, Kröger A (2002) Isolierung der trimeren Hydrogenase aus Wolinella succinogenes durch StrepTactin-Affinitätschromatographie. Biospektrum 1:101
-
(2002)
Biospektrum
, vol.1
, pp. 101
-
-
Groß, R.1
Pisa, R.2
Simon, J.3
Kröger, A.4
-
36
-
-
0025977037
-
Eukaryotic proteins expressed in E. coli: An improved thrombin cleavage and purification procedure of fusion proteins with glutathione S-transferase
-
Guan K, Dixon JE (1991) Eukaryotic proteins expressed in E. coli: an improved thrombin cleavage and purification procedure of fusion proteins with glutathione S-transferase. Anal Biochem 192:262-267
-
(1991)
Anal Biochem
, vol.192
, pp. 262-267
-
-
Guan, K.1
Dixon, J.E.2
-
37
-
-
0035900642
-
Control of intrinsic transcription termination by N and NusA: The basic mechanisms
-
Gusarov I, Nudler E (2001) Control of intrinsic transcription termination by N and NusA: the basic mechanisms. Cell 107:437-449
-
(2001)
Cell
, vol.107
, pp. 437-449
-
-
Gusarov, I.1
Nudler, E.2
-
39
-
-
0035881213
-
Introduction of a (poly)histidine tag in L-lactate dehydrogenase produces a mixture of active and inactive molecules
-
Halliwell CM, Morgan G, Ou CP, Cass AE (2001) Introduction of a (poly)histidine tag in L-lactate dehydrogenase produces a mixture of active and inactive molecules. Anal Biochem 295:257-261
-
(2001)
Anal Biochem
, vol.295
, pp. 257-261
-
-
Halliwell, C.M.1
Morgan, G.2
Ou, C.P.3
Cass, A.E.4
-
40
-
-
0036289016
-
AMP-activated protein kinase: Detection with recombinant AMPK alphal subunit
-
Hamilton SR, O'Donnell JB Jr, Hammet A, Stapleton D, Habinowski SA, Means AR, Kemp BE, Witters LA (2002) AMP-activated protein kinase: detection with recombinant AMPK alphal subunit. Biochem Biophys Res Commun 293:892-898
-
(2002)
Biochem Biophys Res Commun
, vol.293
, pp. 892-898
-
-
Hamilton, S.R.1
O'Donnell J.B., Jr.2
Hammet, A.3
Stapleton, D.4
Habinowski, S.A.5
Means, A.R.6
Kemp, B.E.7
Witters, L.A.8
-
41
-
-
0002366954
-
Purification of recombinant component A of 2-hydroxylglutaryl-CoA dehydratase from Acidaminococcus fermentans using Strep-Tactin affinity-chromatography
-
Hans M, Buckel W (2000) Purification of recombinant component A of 2-hydroxylglutaryl-CoA dehydratase from Acidaminococcus fermentans using Strep-Tactin affinity-chromatography. Biotechnol Intl September: 12
-
(2000)
Biotechnol Intl
, vol.SEPTEMBER
, pp. 12
-
-
Hans, M.1
Buckel, W.2
-
42
-
-
0001960830
-
Expression of soluble heterologous proteins via fusion with NusA protein
-
Harrison RG (2000) Expression of soluble heterologous proteins via fusion with NusA protein. inNovation 11:4-7
-
(2000)
inNovation
, vol.11
, pp. 4-7
-
-
Harrison, R.G.1
-
43
-
-
0026578999
-
Ligation-independent cloning of glutathione fusion genes for expression in Escherichia coli
-
Haun RS, Moss J (1992) Ligation-independent cloning of glutathione fusion genes for expression in Escherichia coli. Gene 112:37-43
-
(1992)
Gene
, vol.112
, pp. 37-43
-
-
Haun, R.S.1
Moss, J.2
-
44
-
-
0001396556
-
A better grip on calmodulin
-
Head JF (1992) A better grip on calmodulin. Curr Biol 2:609-611
-
(1992)
Curr Biol
, vol.2
, pp. 609-611
-
-
Head, J.F.1
-
45
-
-
0035881536
-
A novel purification method for histidine-tagged proteins containing a thrombin cleavage site
-
Hefti MH, Caroline JG, der Toorn VV, Dixon R, Vervoort J (2001) A novel purification method for histidine-tagged proteins containing a thrombin cleavage site. Anal Biochem 295:180-185
-
(2001)
Anal Biochem
, vol.295
, pp. 180-185
-
-
Hefti, M.H.1
Caroline, J.G.2
Der Toorn, V.V.3
Dixon, R.4
Vervoort, J.5
-
46
-
-
0023659137
-
New metal chelate adsorbent selective for proteins and peptide containing neighbouring histidine residues
-
Hochuli E, Döbeli H, Schacher A (1987) New metal chelate adsorbent selective for proteins and peptide containing neighbouring histidine residues. J Chromatogr 411:177-184
-
(1987)
J Chromatogr
, vol.411
, pp. 177-184
-
-
Hochuli, E.1
Döbeli, H.2
Schacher, A.3
-
47
-
-
0023781763
-
Genetic approach to facilitate purification of recombinant proteins with a novel metal chelate adsorbent
-
Hochuli E, Bannwarth W, Döbeli H, Gentz R, Stüber D (1988) Genetic approach to facilitate purification of recombinant proteins with a novel metal chelate adsorbent. Bio/Technology 6:1321-1325
-
(1988)
Bio/Technology
, vol.6
, pp. 1321-1325
-
-
Hochuli, E.1
Bannwarth, W.2
Döbeli, H.3
Gentz, R.4
Stüber, D.5
-
48
-
-
0035866279
-
A novel multiple affinity purification tag and its use in identification of proteins associated a cyclin-CDK complex
-
Honey S, Schneider BL, Schieltz DM, Yates JR, Futcher B (2001) A novel multiple affinity purification tag and its use in identification of proteins associated a cyclin-CDK complex. Nucleic Acids Res 29:e24 (http://nar.oupjournals.org/cgi/content/full/29/4/e24)
-
(2001)
Nucleic Acids Res
, vol.29
-
-
Honey, S.1
Schneider, B.L.2
Schieltz, D.M.3
Yates, J.R.4
Futcher, B.5
-
49
-
-
0030152061
-
Metal-binding properties of a calcium dependent monoclonal antibody
-
Hope TP, Gallis B, Prikett KS (1996) Metal-binding properties of a calcium dependent monoclonal antibody. Mol Immunol 33:601-608
-
(1996)
Mol Immunol
, vol.33
, pp. 601-608
-
-
Hope, T.P.1
Gallis, B.2
Prikett, K.S.3
-
50
-
-
0023714467
-
A short polypeptide marker sequence useful for recombinant protein identification and purification
-
Hopp TP, Pricket KS, Price VL, Libby RT, March CJ, Ceretti DP, Urdal DL, Conlon PJ (1988) A short polypeptide marker sequence useful for recombinant protein identification and purification. Bio/Technology 6:1204-1210
-
(1988)
Bio/Technology
, vol.6
, pp. 1204-1210
-
-
Hopp, T.P.1
Pricket, K.S.2
Price, V.L.3
Libby, R.T.4
March, C.J.5
Ceretti, D.P.6
Urdal, D.L.7
Conlon, P.J.8
-
51
-
-
0033541686
-
Influence of the amino acid residue downstream of (Asp)4Lys on enterokinase cleavage of a fusion protein
-
Hosfield T, Lu Q (1999) Influence of the amino acid residue downstream of (Asp)4Lys on enterokinase cleavage of a fusion protein. Anal Biochem 269:10-16
-
(1999)
Anal Biochem
, vol.269
, pp. 10-16
-
-
Hosfield, T.1
Lu, Q.2
-
52
-
-
0026445630
-
Affinity purification of histidine-tagged proteins transiently produced in HeLa cells
-
Janknecht R, Nordheim A (1992) Affinity purification of histidine-tagged proteins transiently produced in HeLa cells. Gene 121:321-324
-
(1992)
Gene
, vol.121
, pp. 321-324
-
-
Janknecht, R.1
Nordheim, A.2
-
53
-
-
0025946803
-
Rapid and efficient purification of native histidine-tagged protein expressed by recombinant vaccina virus
-
Janknecht R, de Martynoff G, Lou J, Hipskind R, Nordheim A, Stunnenberg HG (1991) Rapid and efficient purification of native histidine-tagged protein expressed by recombinant vaccina virus. Proc Natl Acad Sci USA 88:8972-8976
-
(1991)
Proc Natl Acad Sci USA
, vol.88
, pp. 8972-8976
-
-
Janknecht, R.1
De Martynoff, G.2
Lou, J.3
Hipskind, R.4
Nordheim, A.5
Stunnenberg, H.G.6
-
54
-
-
0034888426
-
Construction, overexpression, and purification of Arthrobacter globiliformis amine oxidase-Strep-Tag II fusion protein
-
Juda CA, Bollinger JA, Dooley DM (2001) Construction, overexpression, and purification of Arthrobacter globiliformis amine oxidase-Strep-Tag II fusion protein. Protein Expr Purif 22:455-461
-
(2001)
Protein Expr Purif
, vol.22
, pp. 455-461
-
-
Juda, C.A.1
Bollinger, J.A.2
Dooley, D.M.3
-
55
-
-
0028107354
-
Formation and properties of S-protein complex with S-peptide-containing fusion protein
-
Karpeisky MY, Senchenko VN, Dianova MV, Kanevsky V (1994) Formation and properties of S-protein complex with S-peptide-containing fusion protein. FEBS Lett 339:209-212
-
(1994)
FEBS Lett
, vol.339
, pp. 209-212
-
-
Karpeisky, M.Y.1
Senchenko, V.N.2
Dianova, M.V.3
Kanevsky, V.4
-
56
-
-
0028353230
-
Production, purification and immunogenicity of a malarian transmission-blocking vaccine candidate: TBV25H expressed in yeast and purified using nickel-NTA agarose
-
Kaslow DC, Shiloach J (1994) Production, purification and immunogenicity of a malarian transmission-blocking vaccine candidate: TBV25H expressed in yeast and purified using nickel-NTA agarose. Bio/Technology 12:494-499
-
(1994)
Bio/Technology
, vol.12
, pp. 494-499
-
-
Kaslow, D.C.1
Shiloach, J.2
-
57
-
-
0025319619
-
Crystal structure of thioredoxin from Escherichia coli at 1.68 Å resolution
-
Katti SK, LeMaster DM, Eklund H (1990) Crystal structure of thioredoxin from Escherichia coli at 1.68 Å resolution. J Mol Biol 212:167-184
-
(1990)
J Mol Biol
, vol.212
, pp. 167-184
-
-
Katti, S.K.1
LeMaster, D.M.2
Eklund, H.3
-
58
-
-
0035543198
-
One-step purification of recombinant proteins using a nanomolar-affinity streptavidin-binding peptide, the SBP-Tag
-
Keefe AD, Wilson DS, Seelig B, Szostak JW (2001) One-step purification of recombinant proteins using a nanomolar-affinity streptavidin-binding peptide, the SBP-Tag. Protein Expr Purif 23:440-446
-
(2001)
Protein Expr Purif
, vol.23
, pp. 440-446
-
-
Keefe, A.D.1
Wilson, D.S.2
Seelig, B.3
Szostak, J.W.4
-
59
-
-
0033568093
-
Hypersensitive substrate for ribonucleases
-
Kelemen BR, Klink TA, Behlke MA, Eubanks SR, Leland PA, Raines RT (1999) Hypersensitive substrate for ribonucleases. Nucleic Acids Res 27:3696-3701
-
(1999)
Nucleic Acids Res
, vol.27
, pp. 3696-3701
-
-
Kelemen, B.R.1
Klink, T.A.2
Behlke, M.A.3
Eubanks, S.R.4
Leland, P.A.5
Raines, R.T.6
-
60
-
-
0028087744
-
A misfolded but active dimer of bovine seminal ribonuclease
-
Kim JS, Raines RT (1994) A misfolded but active dimer of bovine seminal ribonuclease. Eur J Biochem 224:109-114
-
(1994)
Eur J Biochem
, vol.224
, pp. 109-114
-
-
Kim, J.S.1
Raines, R.T.2
-
61
-
-
0029793209
-
Rapid detection of recombinant antibody fragments directed against cell-surface antigens by flow cytometry
-
Kipriyanov SM, Kupriyanova OA, Little M, Moldenhauer G (1996) Rapid detection of recombinant antibody fragments directed against cell-surface antigens by flow cytometry. J Immunol Methods 196:51-62
-
(1996)
J Immunol Methods
, vol.196
, pp. 51-62
-
-
Kipriyanov, S.M.1
Kupriyanova, O.A.2
Little, M.3
Moldenhauer, G.4
-
62
-
-
0027524866
-
The cystic fibrosis transmembrane conductance regulator. Overexpression, purification, and characterization of wild type and delta F508 mutant forms of the first nucleotide binding fold in fusion with the maltose-binding protein
-
Ko YH, Thomas PJ, Delannoy MR, Pedersen PL (1993) The cystic fibrosis transmembrane conductance regulator. Overexpression, purification, and characterization of wild type and delta F508 mutant forms of the first nucleotide binding fold in fusion with the maltose-binding protein. J Biol Chem 268:24330-24338
-
(1993)
J Biol Chem
, vol.268
, pp. 24330-24338
-
-
Ko, Y.H.1
Thomas, P.J.2
Delannoy, M.R.3
Pedersen, P.L.4
-
63
-
-
0036129945
-
Improved affinity of engineered Streptavidin for the Strep-tag II peptide is due to a fixed open conformation of the lid-like loop at the binding site
-
Korndörfer IP, Skerra A (2001) Improved affinity of engineered Streptavidin for the Strep-tag II peptide is due to a fixed open conformation of the lid-like loop at the binding site. Protein Sci 11:883-893
-
(2001)
Protein Sci
, vol.11
, pp. 883-893
-
-
Korndörfer, I.P.1
Skerra, A.2
-
64
-
-
0026801193
-
The human leukocyte platelet-activating factor receptor
-
Kunz D, Gerad NP, Gerad C (1992) The human leukocyte platelet-activating factor receptor. J Biol Chem 267:9101-9106
-
(1992)
J Biol Chem
, vol.267
, pp. 9101-9106
-
-
Kunz, D.1
Gerad, N.P.2
Gerad, C.3
-
65
-
-
0028811610
-
Purification of recombinant GluR-D glutamate receptor produced in Sf21 insect cells
-
Kuusinen A, Arvola M, Oker-Blom C, Keinämen K (1995) Purification of recombinant GluR-D glutamate receptor produced in Sf21 insect cells. Eur J Biochem 233:720-726
-
(1995)
Eur J Biochem
, vol.233
, pp. 720-726
-
-
Kuusinen, A.1
Arvola, M.2
Oker-Blom, C.3
Keinämen, K.4
-
66
-
-
0024448304
-
MyoD is a sequence-specific DNA binding protein requiring a region of myc homology to bind to the muscle creatine kinase enhancer
-
Lassar AB, Buskin JN, Lockshon D, Davis RL, Apone S, Hauschka SD, Weintraub H (1989) MyoD is a sequence-specific DNA binding protein requiring a region of myc homology to bind to the muscle creatine kinase enhancer. Cell 58:823-831
-
(1989)
Cell
, vol.58
, pp. 823-831
-
-
Lassar, A.B.1
Buskin, J.N.2
Lockshon, D.3
Davis, R.L.4
Apone, S.5
Hauschka, S.D.6
Weintraub, H.7
-
67
-
-
0011962568
-
Thioredoxin as a fusion partner for production of soluble recombinant proteins in Escherichia coli
-
LaVallie ER, Lu Z, Diblasimo-Smith EA, Collins-Racie LA, McCoy JM (2000) Thioredoxin as a fusion partner for production of soluble recombinant proteins in Escherichia coli. Methods Enzymol 326:322-340
-
(2000)
Methods Enzymol
, vol.326
, pp. 322-340
-
-
LaVallie, E.R.1
Lu, Z.2
Diblasimo-Smith, E.A.3
Collins-Racie, L.A.4
McCoy, J.M.5
-
68
-
-
0033058619
-
Expression and study of recombinant ExoM, a betal-4 glucosyltransferase involved in succinoglycan biosynthesis in Sinorhizobium meliloti
-
Lellouch AC, Geremia RA (1999) Expression and study of recombinant ExoM, a betal-4 glucosyltransferase involved in succinoglycan biosynthesis in Sinorhizobium meliloti. J Bacteriol 181:1141-1148
-
(1999)
J Bacteriol
, vol.181
, pp. 1141-1148
-
-
Lellouch, A.C.1
Geremia, R.A.2
-
69
-
-
0030728354
-
Using Schizosaccharomyces pombe as host for expression and purification of eukaryotic proteins
-
Lu Q, Bauer JC, Greener A (1997) Using Schizosaccharomyces pombe as host for expression and purification of eukaryotic proteins. Gene 200:135-144
-
(1997)
Gene
, vol.200
, pp. 135-144
-
-
Lu, Q.1
Bauer, J.C.2
Greener, A.3
-
70
-
-
0029146150
-
Cloning vectors for the production of proteins in Dictyostelium discoideum
-
Manstein DJ, Schuster H-P, Morandini P, Hunt DM (1995) Cloning vectors for the production of proteins in Dictyostelium discoideum. Gene 162:129-134
-
(1995)
Gene
, vol.162
, pp. 129-134
-
-
Manstein, D.J.1
Schuster, H.-P.2
Morandini, P.3
Hunt, D.M.4
-
71
-
-
0015239936
-
Purification and specificity of procine enterokinase
-
Maroux S, Baratti J, Desnuelle P (1971) Purification and specificity of procine enterokinase. J Biol Chem 246:5031-5039
-
(1971)
J Biol Chem
, vol.246
, pp. 5031-5039
-
-
Maroux, S.1
Baratti, J.2
Desnuelle, P.3
-
72
-
-
0026059615
-
The noncatalytic src homology 2 segment of abl tyrosine kinase binds to tyrosine-phosphorylated cellular proteins with high affinity
-
Mayer BJ, Jackson PK, Baltimore D (1991) The noncatalytic src homology 2 segment of abl tyrosine kinase binds to tyrosine-phosphorylated cellular proteins with high affinity. Proc Natl Acad Sci USA 88:627-631
-
(1991)
Proc Natl Acad Sci USA
, vol.88
, pp. 627-631
-
-
Mayer, B.J.1
Jackson, P.K.2
Baltimore, D.3
-
73
-
-
12244294110
-
Purification and S-Tag detection of CBD fusion proteins
-
McCormick M, Berg J (1997) Purification and S-Tag detection of CBD fusion proteins. inNovations 7:12-15
-
(1997)
inNovations
, vol.7
, pp. 12-15
-
-
McCormick, M.1
Berg, J.2
-
74
-
-
0031456271
-
Crystallization of the first three domain of the human insuline-like growth factor-1 receptor
-
McKern NM, Lou M, Frenkel MJ, Verkuylen A, Bentley JD, Lovrecz GO, Ivancic N, Elleman TC, Garrett TP, Cosgrove LJ, Ward CW (1997) Crystallization of the first three domain of the human insuline-like growth factor-1 receptor. Protein Sci 6:2663-2666
-
(1997)
Protein Sci
, vol.6
, pp. 2663-2666
-
-
McKern, N.M.1
Lou, M.2
Frenkel, M.J.3
Verkuylen, A.4
Bentley, J.D.5
Lovrecz, G.O.6
Ivancic, N.7
Elleman, T.C.8
Garrett, T.P.9
Cosgrove, L.J.10
Ward, C.W.11
-
75
-
-
0028931691
-
Chrystal structures of a Schistosomal drug and vaccine target: Glutathione S-transferase from Schistosoma japonica and its complex with the leading antischistosomal drug praziquantel
-
McTigue MA, Williams DR, Tainer JA (1995) Chrystal structures of a Schistosomal drug and vaccine target: glutathione S-transferase from Schistosoma japonica and its complex with the leading antischistosomal drug praziquantel. J Mol Biol 246:21-27
-
(1995)
J Mol Biol
, vol.246
, pp. 21-27
-
-
McTigue, M.A.1
Williams, D.R.2
Tainer, J.A.3
-
76
-
-
0033563786
-
Expression of a Cx43 deletion mutant in 3T3 A31 fibroblasts prevents PDGF-induced inhibition of cell communication and suppress cell growth
-
Moorby CD, Gherardi E (1999) Expression of a Cx43 deletion mutant in 3T3 A31 fibroblasts prevents PDGF-induced inhibition of cell communication and suppress cell growth. Exp Cell Res 249:367-376
-
(1999)
Exp Cell Res
, vol.249
, pp. 367-376
-
-
Moorby, C.D.1
Gherardi, E.2
-
77
-
-
0023052239
-
An Hsp70-like protein in the ER: Identity with the 78 kd glucose-regulated protein and immunoglobulin heavy chain binding protein
-
Munro S, Pelham HRB (1986) An Hsp70-like protein in the ER: identity with the 78 kd glucose-regulated protein and immunoglobulin heavy chain binding protein. Cell 46:291-300
-
(1986)
Cell
, vol.46
, pp. 291-300
-
-
Munro, S.1
Pelham, H.R.B.2
-
78
-
-
0031128201
-
Purification and characterization of recombinant affinity peptide-tagged oat phytochrom A
-
Murphy JT, Lagarias JC (1997) Purification and characterization of recombinant affinity peptide-tagged oat phytochrom A. Photochem Photobiol 65:750-758
-
(1997)
Photochem Photobiol
, vol.65
, pp. 750-758
-
-
Murphy, J.T.1
Lagarias, J.C.2
-
79
-
-
0021153567
-
Generation of β-globin by sequence-specific proteolysis of a hybrid protein produced in Escherichia coli
-
Nagai K, Thogersen HC (1984) Generation of β-globin by sequence-specific proteolysis of a hybrid protein produced in Escherichia coli. Nature 309:810-812
-
(1984)
Nature
, vol.309
, pp. 810-812
-
-
Nagai, K.1
Thogersen, H.C.2
-
80
-
-
0023517015
-
Synthesis and sequence specific proteolysis of hybrid proteins produced in Escherichia coli
-
Nagai K, Thogersen HC (1987) Synthesis and sequence specific proteolysis of hybrid proteins produced in Escherichia coli. Methods Enzymol 153:461-481
-
(1987)
Methods Enzymol
, vol.153
, pp. 461-481
-
-
Nagai, K.1
Thogersen, H.C.2
-
81
-
-
0022053837
-
Immobilization and purification of enzymes with staphylococcal protein A gene fusion vectors
-
Nilsson B, Abrahmsén L, Uhlén M. (1985) Immobilization and purification of enzymes with staphylococcal protein A gene fusion vectors. EMBO J 4:1075-1080
-
(1985)
EMBO J
, vol.4
, pp. 1075-1080
-
-
Nilsson, B.1
Abrahmsén, L.2
Uhlén, M.3
-
82
-
-
0031559948
-
Reversible, site-specific immobilization of polyarginine-tagged fusion proteins on mica surfaces
-
Nock S, Spudich JA, Wagner P (1997) Reversible, site-specific immobilization of polyarginine-tagged fusion proteins on mica surfaces. FEBS Lett 414:233-238
-
(1997)
FEBS Lett
, vol.414
, pp. 233-238
-
-
Nock, S.1
Spudich, J.A.2
Wagner, P.3
-
83
-
-
0028872393
-
vfragment against an integral membrane protein diffracting to 1.28 Å resolution
-
vfragment against an integral membrane protein diffracting to 1.28 Å resolution. Proteins 21:74-77
-
(1995)
Proteins
, vol.21
, pp. 74-77
-
-
Ostermeier, C.1
Essen, L.-O.2
Michel, H.3
-
86
-
-
0028329918
-
Release of proteins and peptides from fusion proteins using a recombinant plant virus proteinase
-
Parks TD, Leuther KK, Howard ED, Johnston SA, Dougherty WG (1994) Release of proteins and peptides from fusion proteins using a recombinant plant virus proteinase. Anal Biochem 216:413-417
-
(1994)
Anal Biochem
, vol.216
, pp. 413-417
-
-
Parks, T.D.1
Leuther, K.K.2
Howard, E.D.3
Johnston, S.A.4
Dougherty, W.G.5
-
87
-
-
0036268443
-
Purification and characterization of VanXYC, a d,d-dipeptidase/d,d-carboxypeptidase in vancomycin-resistant Enterococcus gallinarum BM4174
-
Podmore AH, Reynolds PE (2002) Purification and characterization of VanXYC, a d,d-dipeptidase/d,d-carboxypeptidase in vancomycin-resistant Enterococcus gallinarum BM4174. Eur J Biochem 269:2740-2746
-
(2002)
Eur J Biochem
, vol.269
, pp. 2740-2746
-
-
Podmore, A.H.1
Reynolds, P.E.2
-
88
-
-
0016717761
-
Metal chelate affinity chromatography, a new approach to protein fractionation
-
Porath J, Carlsson J, Olsson I, Belfrage G (1975) Metal chelate affinity chromatography, a new approach to protein fractionation. Nature 258:598-599
-
(1975)
Nature
, vol.258
, pp. 598-599
-
-
Porath, J.1
Carlsson, J.2
Olsson, I.3
Belfrage, G.4
-
89
-
-
0031214792
-
6-tag and maltose-binding-protein double-affinity system
-
6-tag and maltose-binding-protein double-affinity system. Prot Expr Purif 10:309-319
-
(1997)
Prot Expr Purif
, vol.10
, pp. 309-319
-
-
Pryor, K.D.1
Leiting, B.2
-
90
-
-
0034841844
-
The tandem affinity purification (TAP) method: A general procedure of protein complex purification
-
Puig O, Caspary F, Rigaut G, Rutz B, Bouveret E, Bragado-Nilsson E, Wilm M, Seraphin B (2001) The tandem affinity purification (TAP) method: a general procedure of protein complex purification. Methods 24:218-229
-
(2001)
Methods
, vol.24
, pp. 218-229
-
-
Puig, O.1
Caspary, F.2
Rigaut, G.3
Rutz, B.4
Bouveret, E.5
Bragado-Nilsson, E.6
Wilm, M.7
Seraphin, B.8
-
91
-
-
0034962756
-
[W206R]-procaspase 3: An inactivateable substrate for caspase 8
-
Rank KB, Mildner AM, Leone JW, Koeplinger KA, Chou KC, Tomasselli AG, Heinrikson RL, Sharma SK (2001). [W206R]-procaspase 3: an inactivateable substrate for caspase 8. Protein Expr Purif 22:258-266
-
(2001)
Protein Expr Purif
, vol.22
, pp. 258-266
-
-
Rank, K.B.1
Mildner, A.M.2
Leone, J.W.3
Koeplinger, K.A.4
Chou, K.C.5
Tomasselli, A.G.6
Heinrikson, R.L.7
Sharma, S.K.8
-
92
-
-
0032828715
-
A generic protein purification method for protein complex characterization and proteome exploration
-
Rigaut G, Shevchenko A, Rutz B, Wilm M, Mann M, Seraphin B (1999) A generic protein purification method for protein complex characterization and proteome exploration. Nat Biotechnol 17:1030-1032
-
(1999)
Nat Biotechnol
, vol.17
, pp. 1030-1032
-
-
Rigaut, G.1
Shevchenko, A.2
Rutz, B.3
Wilm, M.4
Mann, M.5
Seraphin, B.6
-
93
-
-
0029107830
-
Improved factor Xa cleavage of fusion proteins containing maltose binding protein
-
Rodriguez PL, Carrasco L (1995) Improved factor Xa cleavage of fusion proteins containing maltose binding protein. Biotechniques 238:241-243
-
(1995)
Biotechniques
, vol.238
, pp. 241-243
-
-
Rodriguez, P.L.1
Carrasco, L.2
-
94
-
-
0027052982
-
pGSTag - A versatile bacterial expression Plasmid for enzymatic labelling of recombinant proteins
-
Ron D, Dressler H (1992) pGSTag - a versatile bacterial expression Plasmid for enzymatic labelling of recombinant proteins. Biotechniques 13:866-869
-
(1992)
Biotechniques
, vol.13
, pp. 866-869
-
-
Ron, D.1
Dressler, H.2
-
95
-
-
0029998992
-
pLEF, a novel vector for expression of glutathione S-transferase fusion proteins in mammalian cells
-
Rudert F, Visser E, Gradl G, Grandison P, Shemshedini L, Wang Y, Grierson A, Watson J (1996) pLEF, a novel vector for expression of glutathione S-transferase fusion proteins in mammalian cells. Gene 169:281-282
-
(1996)
Gene
, vol.169
, pp. 281-282
-
-
Rudert, F.1
Visser, E.2
Gradl, G.3
Grandison, P.4
Shemshedini, L.5
Wang, Y.6
Grierson, A.7
Watson, J.8
-
96
-
-
53149124391
-
Properties of soluble fusions between mammalian aspartic proteinases and bacterial maltose-binding protein
-
Sachdev D, Chirgwin JM (1999) Properties of soluble fusions between mammalian aspartic proteinases and bacterial maltose-binding protein. J Protein Chem 18:127-136
-
(1999)
J Protein Chem
, vol.18
, pp. 127-136
-
-
Sachdev, D.1
Chirgwin, J.M.2
-
97
-
-
0033809233
-
Fusions to maltose-binding protein: Control of folding and solubility in protein purification
-
Sachdev D, Chirgwin JM (2000) Fusions to maltose-binding protein: control of folding and solubility in protein purification. Methods Enzymol 326:312-321
-
(2000)
Methods Enzymol
, vol.326
, pp. 312-321
-
-
Sachdev, D.1
Chirgwin, J.M.2
-
98
-
-
0037128650
-
Epidermal transglutaminase (TGase 3) is the autoantigen of dermatitis herpetiformis
-
Sárdy M, Kárpáti S, Merkl B, Paulsson M, Smyth N (2002) Epidermal transglutaminase (TGase 3) is the autoantigen of dermatitis herpetiformis. J Exp Med 195:747-757
-
(2002)
J Exp Med
, vol.195
, pp. 747-757
-
-
Sárdy, M.1
Kárpáti, S.2
Merkl, B.3
Paulsson, M.4
Smyth, N.5
-
99
-
-
0021320171
-
A polypeptide fusion designed for purification of recombinant proteins
-
Sassenfeld HM, Brewer SJ (1984) A polypeptide fusion designed for purification of recombinant proteins. Bio/Technology 2:76-81
-
(1984)
Bio/Technology
, vol.2
, pp. 76-81
-
-
Sassenfeld, H.M.1
Brewer, S.J.2
-
100
-
-
0039310043
-
Use of peptide libraries to map the substrate specificity of a peptide-modifying enzyme: A 13 residue consensus peptide specifies biotinylation in Escherichia coli
-
Schatz PJ (1993) Use of peptide libraries to map the substrate specificity of a peptide-modifying enzyme: a 13 residue consensus peptide specifies biotinylation in Escherichia coli. Bio/Technology 11:1138-1143
-
(1993)
Bio/Technology
, vol.11
, pp. 1138-1143
-
-
Schatz, P.J.1
-
101
-
-
0242414867
-
Expression of functional melanocorcin 1 receptor in insect cells
-
Schioth HB, Kuusinen A, Muceniece R, Szardenings M, Keinanen K, Wikberg JE (1996) Expression of functional melanocorcin 1 receptor in insect cells. Bichem Biophys Res Commun 221:807-814
-
(1996)
Bichem Biophys Res Commun
, vol.221
, pp. 807-814
-
-
Schioth, H.B.1
Kuusinen, A.2
Muceniece, R.3
Szardenings, M.4
Keinanen, K.5
Wikberg, J.E.6
-
102
-
-
0032052447
-
Baculovirus-mediated large-scale expression and purification of a polyhistidine-tagged Rubrella virus capsid protein
-
Schmidt M, Tuominen N, Johansson T, Weiss SA, Keinamen K, Oker-Blom C (1998) Baculovirus-mediated large-scale expression and purification of a polyhistidine-tagged Rubrella virus capsid protein. Protein Expr Purif 12:323-330
-
(1998)
Protein Expr Purif
, vol.12
, pp. 323-330
-
-
Schmidt, M.1
Tuominen, N.2
Johansson, T.3
Weiss, S.A.4
Keinamen, K.5
Oker-Blom, C.6
-
103
-
-
0027499708
-
The random peptide library-assisted engineering of a C-terminal affinity peptide, useful for the detection and purification of a functional Ig Fv fragment
-
Schmidt TGM, Skerra A (1993) The random peptide library-assisted engineering of a C-terminal affinity peptide, useful for the detection and purification of a functional Ig Fv fragment. Protein Eng 6:109-122
-
(1993)
Protein Eng
, vol.6
, pp. 109-122
-
-
Schmidt, T.G.M.1
Skerra, A.2
-
104
-
-
0029865819
-
Molecular interaction between the Strep-tag affinity peptide and its cognate target, Streptavidin
-
Schmidt TGM, Koepke J, Frank R, Skerra A (1996) Molecular interaction between the Strep-tag affinity peptide and its cognate target, Streptavidin. J Mol Biol 255:753-766
-
(1996)
J Mol Biol
, vol.255
, pp. 753-766
-
-
Schmidt, T.G.M.1
Koepke, J.2
Frank, R.3
Skerra, A.4
-
105
-
-
0034097182
-
Protein expression strategies for identification of novel target proteins
-
Schuster M, Wasserbauer E, Einhauer A, Ordner C, Jungbauer A, Hammerschmidt F, Werner G (2000) Protein expression strategies for identification of novel target proteins. J Biomol Screen 5:89-97
-
(2000)
J Biomol Screen
, vol.5
, pp. 89-97
-
-
Schuster, M.1
Wasserbauer, E.2
Einhauer, A.3
Ordner, C.4
Jungbauer, A.5
Hammerschmidt, F.6
Werner, G.7
-
106
-
-
0028842924
-
Yeast aminopeptidase I is post-translationally sorted from the cytosol to the vacuole by a mechanism mediated by its bipartite N-terminal extension
-
Sequi-Real B, Martinez M, Sandoval IV (1995) Yeast aminopeptidase I is post-translationally sorted from the cytosol to the vacuole by a mechanism mediated by its bipartite N-terminal extension. EMBO J 14:5476-5484
-
(1995)
EMBO J
, vol.14
, pp. 5476-5484
-
-
Sequi-Real, B.1
Martinez, M.2
Sandoval, I.V.3
-
107
-
-
0023806075
-
Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase
-
Smith DB, Johnson K (1988) Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase. Gene 67:31-40
-
(1988)
Gene
, vol.67
, pp. 31-40
-
-
Smith, D.B.1
Johnson, K.2
-
108
-
-
0033657698
-
Eucaryotic expression and purification of recombinant extracellular matrix proteins carrying the Strep II tag
-
Streuli CH, Grant ME (eds). Humana, Totowa, New Jersey
-
Smyth N, Odenthal U, Merkl B, Paulsson M (2000) Eucaryotic expression and purification of recombinant extracellular matrix proteins carrying the Strep II tag. In: Streuli CH, Grant ME (eds) Extracellular matrix protocols. Humana, Totowa, New Jersey, pp 49-57
-
(2000)
Extracellular Matrix Protocols
, pp. 49-57
-
-
Smyth, N.1
Odenthal, U.2
Merkl, B.3
Paulsson, M.4
-
109
-
-
0026577811
-
A single step purification for recombinant proteins
-
Stofko-Hahn RE, Carr DW, Scott JD (1992) A single step purification for recombinant proteins. FEBS Lett 302:274-278
-
(1992)
FEBS Lett
, vol.302
, pp. 274-278
-
-
Stofko-Hahn, R.E.1
Carr, D.W.2
Scott, J.D.3
-
110
-
-
0026496171
-
Production of recombinant porcine tumor necrosis factor alpha in a novel E. coli expression system
-
Su XA, Prestwood AK, McGraw RA (1992) Production of recombinant porcine tumor necrosis factor alpha in a novel E. coli expression system. BioTechniques 13:756-762
-
(1992)
BioTechniques
, vol.13
, pp. 756-762
-
-
Su, X.A.1
Prestwood, A.K.2
McGraw, R.A.3
-
111
-
-
0028830219
-
A modified pGEX expression system that eliminates degradation products and thrombin from the recombinant protein
-
Sun G, Budde RJA (1995) A modified pGEX expression system that eliminates degradation products and thrombin from the recombinant protein. Anal Biochem 231:458-460
-
(1995)
Anal Biochem
, vol.231
, pp. 458-460
-
-
Sun, G.1
Budde, R.J.A.2
-
112
-
-
0034881178
-
Cloning, expression, and purification of the Staphylococcus simulans lysostaphin using the intein-chitin-binding domain (CBD) system
-
Sweda P, Pladzyk R, Kotlowski R, Kur J (2001) Cloning, expression, and purification of the Staphylococcus simulans lysostaphin using the intein-chitin-binding domain (CBD) system. Protein Expr Purif 22:467-471
-
(2001)
Protein Expr Purif
, vol.22
, pp. 467-471
-
-
Sweda, P.1
Pladzyk, R.2
Kotlowski, R.3
Kur, J.4
-
113
-
-
0010461149
-
Laboratory and field evaluation of defined antigen vaccines against Schistosoma bovis and S. japonicum in animals and of defined antigens for the immunodiagnosis of human S. japonicum infection
-
Jepsen S, Hagan P, Klein R, Taylor D (eds). Summaries of the final reports of the research contracts. Parasitology European Commission DGXII, A
-
Taylor MG, Bushara H, Capron A, Xu S, Butterworth A (1994) Laboratory and field evaluation of defined antigen vaccines against Schistosoma bovis and S. japonicum in animals and of defined antigens for the immunodiagnosis of human S. japonicum infection. In: Jepsen S, Hagan P, Klein R, Taylor D (eds). Science and technology for development-health-second programme (1987-1991). Summaries of the final reports of the research contracts. Parasitology European Commission DGXII, A: 189-200
-
(1994)
Science and Technology for Development-Health-Second Programme (1987-1991)
, pp. 189-200
-
-
Taylor, M.G.1
Bushara, H.2
Capron, A.3
Xu, S.4
Butterworth, A.5
-
114
-
-
0032508619
-
Characterization and affinity applications of cellulose-binding domains
-
Tomme P, Boraston A, McLean B, Kormos J, Creagh AL, Sturch K, Gilkes NR, Haynes CA, Warren RA, Kilburn DG (1998) Characterization and affinity applications of cellulose-binding domains. J Chromatogr B 715:283-296
-
(1998)
J Chromatogr B
, vol.715
, pp. 283-296
-
-
Tomme, P.1
Boraston, A.2
McLean, B.3
Kormos, J.4
Creagh, A.L.5
Sturch, K.6
Gilkes, N.R.7
Haynes, C.A.8
Warren, R.A.9
Kilburn, D.G.10
-
115
-
-
0029838532
-
Purification of a rat neurotensin receptor expressed in Escherichia coli
-
Tucker J, Grisshammer R (1996) Purification of a rat neurotensin receptor expressed in Escherichia coli. Biochem J 317:891-899
-
(1996)
Biochem J
, vol.317
, pp. 891-899
-
-
Tucker, J.1
Grisshammer, R.2
-
116
-
-
0020602654
-
Gene fusion vectors based on the gene for staphylococcal protein A
-
Uhlén M, Nilsson B, Guss B, Lindberg M, Gatenbeck S, Philipson L (1983) Gene fusion vectors based on the gene for staphylococcal protein A. Gene 23:369-378
-
(1983)
Gene
, vol.23
, pp. 369-378
-
-
Uhlén, M.1
Nilsson, B.2
Guss, B.3
Lindberg, M.4
Gatenbeck, S.5
Philipson, L.6
-
117
-
-
0033809234
-
Affinity purification of recombinant proteins fused to calmodulin or calmodulin-binding peptides
-
Vaillancourt P, Zheng C-F, Hoang DQ, Breister L (2000) Affinity purification of recombinant proteins fused to calmodulin or calmodulin-binding peptides. Methods Enzymol 326:340-362
-
(2000)
Methods Enzymol
, vol.326
, pp. 340-362
-
-
Vaillancourt, P.1
Zheng, C.-F.2
Hoang, D.Q.3
Breister, L.4
-
118
-
-
9344223986
-
Human antibodies with sub-nanomolar affinities isolated from large non-immunized phage display library
-
Vaughan, TJ Williams AJ, Pritchard K, Osbourn JK, Pope AR, Earnshaw JC, McCafferty J, Hodits RA, Wilton J, Johnson KS (1996) Human antibodies with sub-nanomolar affinities isolated from large non-immunized phage display library. Nat Biotechnol 14:309-314
-
(1996)
Nat Biotechnol
, vol.14
, pp. 309-314
-
-
Vaughan, T.J.1
Williams, A.J.2
Pritchard, K.3
Osbourn, J.K.4
Pope, A.R.5
Earnshaw, J.C.6
McCafferty, J.7
Hodits, R.A.8
Wilton, J.9
Johnson, K.S.10
-
119
-
-
0030780364
-
Mutagenesis of a flexible loop in streptavidin leads to higher affinity for the Strep-tag II peptide and improved performance in recombinant protein purification
-
Voss S, Skerra A (1997) Mutagenesis of a flexible loop in streptavidin leads to higher affinity for the Strep-tag II peptide and improved performance in recombinant protein purification. Protein Eng 10:975-982
-
(1997)
Protein Eng
, vol.10
, pp. 975-982
-
-
Voss, S.1
Skerra, A.2
-
120
-
-
0028145771
-
The role of the C-terminal domain and type III domains of chitinase A1 from bacillus circulans WL-12 in chitin degradation
-
Watanabe T, Ito Y, Yamada T, Hashimoto M, Sekine S, Tanaka H (1994) The role of the C-terminal domain and type III domains of chitinase A1 from bacillus circulans WL-12 in chitin degradation. J Bacteriol 176:4465-4472
-
(1994)
J Bacteriol
, vol.176
, pp. 4465-4472
-
-
Watanabe, T.1
Ito, Y.2
Yamada, T.3
Hashimoto, M.4
Sekine, S.5
Tanaka, H.6
-
121
-
-
0032521005
-
Comparative biochemical and pharmacological characterization of mouse 5HT5A 5-hydroxytryptamine receptor and the human beta2-adrenergic receptor produced in the methylotrophic yeast Pichia pastoris
-
Weiss HM, Haase W, Michel H, Reihlander H (1998) Comparative biochemical and pharmacological characterization of mouse 5HT5A 5-hydroxytryptamine receptor and the human beta2-adrenergic receptor produced in the methylotrophic yeast Pichia pastoris. Biochem J 330:1137-1147
-
(1998)
Biochem J
, vol.330
, pp. 1137-1147
-
-
Weiss, H.M.1
Haase, W.2
Michel, H.3
Reihlander, H.4
-
122
-
-
0034911505
-
Cloning, nucleotide sequences and expression of a hydantoinase and carbamoylase gene from Arthrobacter aurescens DSM 3745 in Escherichia coli and comparison with corresponding genes from Arthrobacter aurescens DSM 3747
-
Wiese A, Wilms B, Syldatk C, Mattes R, Altenbuchner J (2001) Cloning, nucleotide sequences and expression of a hydantoinase and carbamoylase gene from Arthrobacter aurescens DSM 3745 in Escherichia coli and comparison with corresponding genes from Arthrobacter aurescens DSM 3747. Appl Microbiol Biotechnol 55:750-757
-
(2001)
Appl Microbiol Biotechnol
, vol.55
, pp. 750-757
-
-
Wiese, A.1
Wilms, B.2
Syldatk, C.3
Mattes, R.4
Altenbuchner, J.5
-
123
-
-
0035957374
-
The use of mRNA display to select high-affinity protein-binding peptides
-
Wilson DS, Keefe AD, Szostak JW (2001) The use of mRNA display to select high-affinity protein-binding peptides. PNAS 98:3750-3755
-
(2001)
PNAS
, vol.98
, pp. 3750-3755
-
-
Wilson, D.S.1
Keefe, A.D.2
Szostak, J.W.3
-
124
-
-
0033255928
-
Hexahistidine (His6)-tag dependent protein dimerization: A cautionary tale
-
Wu J, Filutowicz M (1999) Hexahistidine (His6)-tag dependent protein dimerization: a cautionary tale. Acta Biochim Pol 46:591-599
-
(1999)
Acta Biochim Pol
, vol.46
, pp. 591-599
-
-
Wu, J.1
Filutowicz, M.2
-
125
-
-
0033814098
-
Fusions to self-splicing inteins for protein purification
-
Xu M-Q, Paulus H, Chong S (2000) Fusions to self-splicing inteins for protein purification. Methods Enzymol 236:376-418
-
(2000)
Methods Enzymol
, vol.236
, pp. 376-418
-
-
Xu, M.-Q.1
Paulus, H.2
Chong, S.3
-
126
-
-
0036261635
-
Heavy metal removal by novel CBD-EC20 sorbents immobilized on cellulose
-
Xu Z, Bae W, Mulchandani A, Mehra RK, Chen W (2002) Heavy metal removal by novel CBD-EC20 sorbents immobilized on cellulose. Biomacromolecules 3:462-465
-
(2002)
Biomacromolecules
, vol.3
, pp. 462-465
-
-
Xu, Z.1
Bae, W.2
Mulchandani, A.3
Mehra, R.K.4
Chen, W.5
-
127
-
-
0028826926
-
Cloning, gene sequencing, and expression of the small molecular mass ubiquinone-binding protein of mitochondrial ubiquinol-cytochrom c reductase
-
Yu L, Deng K-P, Yu C-A (1995) Cloning, gene sequencing, and expression of the small molecular mass ubiquinone-binding protein of mitochondrial ubiquinol-cytochrom c reductase. J Biol Chem 270:25634-25638
-
(1995)
J Biol Chem
, vol.270
, pp. 25634-25638
-
-
Yu, L.1
Deng, K.-P.2
Yu, C.-A.3
-
128
-
-
0025986117
-
Novel pathway for thyroid hormone receptor action through interaction with jun and fos oncogene activities
-
Zhang XK, Wills KN, Hunsmann M, Hermann T, Pfahl M (1991) Novel pathway for thyroid hormone receptor action through interaction with jun and fos oncogene activities. Mol Biol 11:6016-6025
-
(1991)
Mol Biol
, vol.11
, pp. 6016-6025
-
-
Zhang, X.K.1
Wills, K.N.2
Hunsmann, M.3
Hermann, T.4
Pfahl, M.5
-
129
-
-
0031579473
-
A new expression vector for high level protein production, one step purification and direct isotopic labeling of calmodulin-binding peptide fusion proteins
-
Zheng C-F, Simcox T, Xu L, Vaillancourt P (1997) A new expression vector for high level protein production, one step purification and direct isotopic labeling of calmodulin-binding peptide fusion proteins. Gene 186:55-60
-
(1997)
Gene
, vol.186
, pp. 55-60
-
-
Zheng, C.-F.1
Simcox, T.2
Xu, L.3
Vaillancourt, P.4
|