Production of recombinant peptides as fusions with SUMO

Protein Expression and Purification

Volumn 78, Issue 2, 2011, Pages 113-119

  Satakarni, Makkapati ^a   Curtis, Robin ^a  

^a UNIVERSITY OF MANCHESTER   (United Kingdom)

Author keywords

Amyloidogenic A 42; Fusion protein; Hydrolase; SUMO

Indexed keywords

AMYLOID BETA PROTEIN; AMYLOID BETA PROTEIN (1 42); AMYLOID BETA-PROTEIN (1-42); HYBRID PROTEIN; OMPA OUTER MEMBRANE PROTEINS; OUTER MEMBRANE PROTEIN; PEPTIDE; PEPTIDE FRAGMENT; PEPTIDE HYDROLASE; SIGNAL PEPTIDE; SUMO 1 PROTEIN;

AFFINITY CHROMATOGRAPHY; ARTICLE; BIOSYNTHESIS; CHEMISTRY; ESCHERICHIA COLI; INTRACELLULAR SPACE; ISOLATION AND PURIFICATION; MASS SPECTROMETRY; METABOLISM; METHODOLOGY; MOLECULAR WEIGHT; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN ENGINEERING; SOLUBILITY;

AMYLOID BETA-PEPTIDES; BACTERIAL OUTER MEMBRANE PROTEINS; CHROMATOGRAPHY, AFFINITY; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; INTRACELLULAR SPACE; MASS SPECTROMETRY; MOLECULAR WEIGHT; PEPTIDE FRAGMENTS; PEPTIDE HYDROLASES; PEPTIDES; PROTEIN ENGINEERING; PROTEIN SORTING SIGNALS; RECOMBINANT FUSION PROTEINS; SOLUBILITY; SUMO-1 PROTEIN;

ANIMALIA; ESCHERICHIA COLI;

EID: 79958768615     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2011.04.015     Document Type: Article

References (38)

1. H. Roder, M.R. Shastry, Methods for exploring early events in protein folding, Curr. Opin. Struct. Biol. 9 (1999) 620-626. (Pubitemid 29460590)
2. R. Novak, B. Henriques, E. Charpentier, S. Normark, E. Tuomanen, Emergence of vancomycin tolerance in Streptococcus pneumoniae, Nature 399 (1999) 590-593.
3. D.G. Lynn, S.C. Meredith, Review: Model peptides and the physicochemical approach to beta-amyloids, J. Struct. Biol. 130 (2000) 153-173.
4. S.G. Zhang, D.M. Marini, W. Hwang, S. Santoso, Design of nanostructured biological materials through self-assembly of peptides and proteins, Curr. Opin. Chem. Biol. 6 (2002) 865-871. (Pubitemid 35449349)
5. F.A. O Marston, The purification of eukaryotic polypeptides synthesized in Escherichia coli, Biochem. J. 240 (1986) 1-12.
6. G. Hannig, S.C. Makrides, Strategies for optimizing heterologous protein expression in Escherichia coli, TIBTECH 16 (1998) 2-9.
7. I. Cipakova, J. Gasperik, E. Hostinova, Expression and purification of human antimicrobial peptide, dermcidin, in Escherichia coli, Protein Express. Purif. 45 (2006) 269-274. (Pubitemid 43120431)
8. G.S. Moon, Y.R. Pyun, W.J. Kim, Expression and purification of a fusion-typed pediocin PA-1 in Escherichia coli and recovery of biologically active pediocin PA-1, Int. J. Food Microbiol. 108 (2006) 136-140.
9. X.M. Fang, L. Peng, Z.N. Xu, J.M. Wu, P.L. Cen, Cloning and expression of β-defensin- 2 gene in Escherichia coli, Prot. Pep. Lett. 9 (2002) 31-37. (Pubitemid 34293914)
10. Y.X. Zhou, W. Cao, Q.P. Luo, Y.S. Ma, J.Z. Wang, D.Z. Wei, Production and purification of a novel antibiotic peptide, adenoregulin, from a recombinant Escherichia coli, Biotechnol. Lett. 27 (2005) 725-730. (Pubitemid 41110932)
11. K. Terpe, Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems, Appl. Microb. Biotechnol. 60 (2003) 523-533. (Pubitemid 36169526)
12. D.B. Smith, K.S. Johnson, Single-step purification of polypeptides expressed In Escherichia coli as fusions with glutathione S-transferase, Gene 67 (1998) 31-40.
13. E.R. LaVallie, J.M. McCoy, Gene fusion expression systems in Escherichia coli, Curr. Opin. Biotechnol. 6 (1995) 501-506.
14. K.D. Pryor, B. Leiting, High-level expression of soluble protein in Escherichia coli using a His(6)-tag and maltose-binding-protein double-affinity fusion system, Protein Express. Purif. 10 (1997) 309-319.
15. M.B. Uhlen, B. Nilsson, B. Guss, M. Lindberg, S. Gatenbeck, L. Philipson, Gene fusion vectors based on the gene for Staphylococcal protein-A, Gene 23 (1983) 369-378. (Pubitemid 13009986)
16. A. Ullmann, One-step purification of hybrid proteins which have beta-galactosidase activity, Gene 29 (1984) 27-31. (Pubitemid 14030929)
17. A.M. Catanzariti, T.A. Soboleva, D.A. Jans, P.G. Board, R.T. Baker, An efficient system for high-level expression and easy purification of authentic recombinant proteins, Prot. Sci. 13 (2004) 1331-1339. (Pubitemid 38526098)
18. T.R. Butt, S. Jonnalagadda, B.P. Monia, E.J. Sternberg, J.A. Marsh, J.M. Stadel, D.J. Ecker, S.T. Crooke, Ubiquitin fusion augments the yield of cloned gene products in Escherichia coli, PNAS USA 86 (1989) 2540-2544. (Pubitemid 19112583)
19. A.R. Welch, C.M. Holman, M.F. Browner, M.R. Gehring, C.C. Kan, H.E. Van Wart, Purification of human matrilysin produced in Escherichia coli and characterization using a new optimized fluorogenic peptide substrate, Arch. Biochem. Biophys. 324 (1995) 59-64.
20. A. Pilon, P. Yost, T.E. Chase, T. Beckett, S. Roberts, W.E. Bentley, Ubiquitin fusion technology: Bioprocessing of peptides, Biotechnol. Prog. 13 (1997) 374-379.
21. E.R. LaVallie, E.A. DiBlasio, S. Kovacic, K.L. Grant, P.F. Schendel, J.M. McCoy, A thioredoxin gene fusion expression system that circumvents inclusion body formation in the Escherichia coli cytoplasm, Bio./Technology 11 (1993) 187-193. (Pubitemid 23056045)
22. R.T. Baker, Protein expression using ubiquitin fusion and cleavage, Curr. Opin. Biotechnol. 7 (1996) 541-546.
23. K.I. Kim, S.H. Baek, S.H. Chung, Versatile protein tag, SUMO: Its enzymology and biological function, J. Cellul. Physiol. 191 (2002) 257-268. (Pubitemid 34462476)
24. T.R. Butt, S.C. Edavettal, J.P. Hall, M.R. Mattern, SUMO fusion technology for difficult-to-express proteins, Protein Express. Purif. 43 (2005) 1-9.
25. M.P. Malakhov, M.R. Mattern, O.A. Malakhova, M. Drinker, S.D. Weeks, T.R. Butt, SUMO fusions and SUMO-specific protease for efficient expression and purification of proteins, J. Struct. Funct. Genom. 5 (2004) 75-86. (Pubitemid 39030623)
26. S.D. Weeks, M. Drinker, P.J. Loll, Ligating independent cloning vectors for expression of SUMO fusions, Protein Express. Purif. 53 (2007) 40-50.
27. C.M. Guzzo, D.C.H. Yang, Systematic analysis of fusion and affinity tags using human-aspartyl-tRNA synthetase expressed in E. Coli, Protein Express. Purif. 54 (2007) 166-175. (Pubitemid 46636330)
28. E. Bosse-Doenecke, U. Weininger, M. Gopalswamy, J. Balbach, S.M. Knudsen, R. Rudolph, High yield production of recombinant native and modified peptides exemplified by ligands for G-protein coupled receptors, Protein Express. Purif. 58 (2008) 114-121.
29. Z. Sun, Z. Xia, J-N. Liu, Expression and purification of human urodilatin by small ubiquitin-related modifier fusion in Escherichia Coli, Appl. Microbiol. Biotechnol. 78 (2008) 495-502.
30. S. Makkapati, Recombinant production of peptides using SUMO as a fusion partner, PhD dissertation, University of Manchester, 2007.
31. C. Haught, G.D. Davis, R. Subramanian, K.W. Jackson, R.G. Harrison, Recombinant production and purification of novel antisense antimicrobial peptide in Escherichia coli, Biotechnol. Bioeng. 57 (1998) 55-61.
32. J.H. Lee, J.H. Kim, S.W. Hwang, W.J. Lee, H.K. Yoon, H.S. Lee, S.S. Hong, High-level expression of antimicrobial peptide mediated by a fusion partner reinforcing formation of inclusion bodies, Biochem. Biophys. Res. Comm. 277 (2000) 575-580.
33. V.S. Skosyrev, E.A. Kulesskiy, L.V. Yakhnin, Y.V. Temirov, L.M. Vinokurov, Expression of the recombinant antibacterial peptide sarcotoxin IA in Escherichia coli cells, Protein Express. Purif. 28 (2003) 350-356.
34. E.K. Lee, J.H. Hwang, D.Y. Shin, D.I. Kim, Y.J. Yoo, Production of recombinant amyloid-β peptide 42 as an ubiquitin extension, Protein Express. Purif. 40 (2005) 183-189.
35. K.W. Miller, R. Schamber, Y.L. Chen, B. Ray, Production of active chimeric pediocin AcH in Escherichia coli in the absence of processing and secretion genes from the Pediococcus pap operon, Appl. Environ. Microbiol. 64 (1998) 1587. (Pubitemid 28188440)
36. Y. Guisez, I. Fache, L.A. Campfield, F.J. Smith, A. Farid, G. Plaetinck, J. Van der Heyden, J. Tavernier, W. Fiers, P. Burn, R. Devos, Efficient secretion of biologically active recombinant OB protein (leptin) in Escherichia coli, purification from the periplasm and characterization, Protein Express. Purif. 12 (1998) 249-258. (Pubitemid 28360451)
37. H.F. Rosenberg, Isolation of recombinant secretory proteins by limited induction and quantitative harvest, Biotechniques 24 (1998) 188-189.
38. A. Shokri, A.M. Sanden, G. Larsson, Growth rate-dependent changes in Escherichia coli membrane structure and protein leakage, Appl. Microb. Biotechnol. 58 (2002) 386-392. (Pubitemid 34185585)