Ubiquitin-intein and SUMO2-intein fusion systems for enhanced protein production and purification

Protein Expression and Purification

Volumn 82, Issue 1, 2012, Pages 174-178

  Wang, Zhongyuan ^a   Li, Na ^a   Wang, Yanyan ^b   Wu, Yanping ^a   Mu, Tianyang ^b   Zheng, Yi ^a   Huang, Laiqiang ^a   Fang, Xuexun ^b  

^a TSINGHUA UNIVERSITY   (China)

^b JILIN UNIVERSITY   (China)

Author keywords

Fusion tags; Intein; Recombinant protein purification; SUMO2; Ubiquitin

Indexed keywords

COLLAGENASE 3; GREEN FLUORESCENT PROTEIN; HIS HIS HIS HIS HIS HIS; HIS-HIS-HIS-HIS-HIS-HIS; HISTIDINE; HYBRID PROTEIN; INTEIN; MMP13 PROTEIN, HUMAN; OLIGOPEPTIDE; SUMO PROTEIN; SUMO2 PROTEIN, HUMAN; UBIQUITIN;

ARTICLE; ESCHERICHIA COLI; GENE EXPRESSION; GENE VECTOR; GENETICS; HUMAN; ISOLATION AND PURIFICATION; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE;

BASE SEQUENCE; ESCHERICHIA COLI; GENE EXPRESSION; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; HISTIDINE; HUMANS; INTEINS; MATRIX METALLOPROTEINASE 13; MOLECULAR SEQUENCE DATA; OLIGOPEPTIDES; RECOMBINANT FUSION PROTEINS; SMALL UBIQUITIN-RELATED MODIFIER PROTEINS; UBIQUITIN;

ESCHERICHIA COLI;

EID: 84862816391     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2011.11.017     Document Type: Article

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