Orthogonal protein purification-Expanding the repertoire of GST fusion systems

Protein Expression and Purification

Volumn 57, Issue 1, 2008, Pages 17-26

  Viljanen, Johan ^a   Larsson, Jenny ^a   Broo, Kerstin S ^b  

^a LINKÖPING UNIVERSITY   (Sweden)

^b UNIVERSITY OF GOTHENBURG   (Sweden)

Author keywords

Fusion partner; Human GST A1 1 mutants; Lysine 216; Methionine 208; Protein purification; Site specific covalent modification

Indexed keywords

ALDEHYDE; COUMARIN; COUMARIN DERIVATIVE; ESTER; FLUORESCENT DYE; GLUTATHIONE S-TRANSFERASE ALPHA; GLUTATHIONE TRANSFERASE; GLUTATHIONE TRANSFERASE ALPHA; HYBRID PROTEIN; ISOENZYME; LYSINE; PEPTIDE HYDROLASE; PEPTIDE LIBRARY; PROTEIN; UNCLASSIFIED DRUG;

ACYLATION; AFFINITY CHROMATOGRAPHY; ARTICLE; BINDING SITE; CATALYSIS; CHEMICAL MODEL; CHEMICAL STRUCTURE; CHEMISTRY; CODON; CRYSTALLIZATION; DIMERIZATION; ENZYME STABILITY; ESCHERICHIA COLI; GENETICS; HUMAN; HYDROLYSIS; HYDROPHOBICITY; ISOLATION AND PURIFICATION; KINETICS; MASS SPECTROMETRY; METABOLISM; MUTATION; NUCLEOTIDE SEQUENCE; PH; PROTEIN BINDING; PROTEIN FOLDING; SITE DIRECTED MUTAGENESIS; TEMPERATURE; TIME;

ACYLATION; ALDEHYDES; BASE SEQUENCE; BINDING SITES; CATALYSIS; CHROMATOGRAPHY, AFFINITY; CODON; COUMARINS; CRYSTALLIZATION; DIMERIZATION; ENZYME STABILITY; ESCHERICHIA COLI; ESTERS; FLUORESCENT DYES; GLUTATHIONE TRANSFERASE; HUMANS; HYDROGEN-ION CONCENTRATION; HYDROLYSIS; HYDROPHOBICITY; ISOENZYMES; KINETICS; LYSINE; MODELS, CHEMICAL; MODELS, MOLECULAR; MOLECULAR STRUCTURE; MUTAGENESIS, SITE-DIRECTED; MUTATION; PEPTIDE HYDROLASES; PEPTIDE LIBRARY; PROTEIN BINDING; PROTEIN FOLDING; PROTEINS; RECOMBINANT FUSION PROTEINS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; TEMPERATURE; TIME FACTORS;

EID: 36249032452     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2007.09.011     Document Type: Article

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