Refolding of lysozyme in hydrophobic interaction chromatography: Effects of hydrophobicity of adsorbent and salt concentration in mobile phase

Biotechnology and Bioprocess Engineering

Volumn 15, Issue 2, 2010, Pages 213-219

  Hwang, Sung Mi ^a   Kang, Hye Jin ^a   Bae, Sang Woo ^a   Chang, Woo Jin ^a   Koo, Yoon Mo ^a  

^a Inha University   (South Korea)

Author keywords

Hydrophobic interaction chromatography (HIC); Protein refolding; Salt

Indexed keywords

AMMONIUM SULFATE; BINDING EFFICIENCY; CONCENTRATION OF; HYDROPHOBIC ENVIRONMENT; HYDROPHOBIC INTERACTION CHROMATOGRAPHY; MOBILE PHASE; OPTIMIZED CONDITIONS; PROTEIN REFOLDING; REFOLDING; SALT CONCENTRATION; SALT SOLUTION; STATIONARY PHASE;

ADSORPTION; AMMONIUM COMPOUNDS; CONCENTRATION (PROCESS); ENZYMES; GEL PERMEATION CHROMATOGRAPHY; HYDROPHOBICITY; OPTIMIZATION; RESINS; UREA;

HYDROPHOBIC CHROMATOGRAPHY;

LYSOZYME; SEPHAROSE; UREA;

ADSORPTION; ARTICLE; DILUTION; ELUTION; ENZYME BINDING; HYDROPHOBIC INTERACTION CHROMATOGRAPHY; HYDROPHOBICITY; PROTEIN FOLDING;

EID: 77953565630     PISSN: 12268372     EISSN: None     Source Type: Journal    
DOI: 10.1007/s12257-009-0216-7     Document Type: Article

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