Optimized expression of soluble cyclomaltodextrinase of thermophilic origin in Escherichia coli by using a soluble fusion-tag and by tuning of inducer concentration

Protein Expression and Purification

Volumn 39, Issue 1, 2005, Pages 54-60

  Turner, Pernilla ^a   Holst, Olle ^a   Karlsson, Eva Nordberg ^a  

^a LUND UNIVERSITY   (Sweden)

Author keywords

Anoxybacillus; Cyclomaltodextrinase; NusA

Indexed keywords

CYCLOMALTODEXTRINASE; ELONGATION FACTOR; ESCHERICHIA COLI PROTEIN; GLYCOSIDASE; HYBRID PROTEIN; NUSA PROTEIN, E COLI; TRANSCRIPTION FACTOR;

ARTICLE; ENZYMOLOGY; ESCHERICHIA COLI; GENE VECTOR; GENETICS; GRAM POSITIVE BACTERIUM; METABOLISM; METHODOLOGY; MOLECULAR CLONING; PLASMID; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROMOTER REGION;

CLONING, MOLECULAR; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENETIC VECTORS; GLYCOSIDE HYDROLASES; GRAM-POSITIVE BACTERIA; PEPTIDE ELONGATION FACTORS; PLASMIDS; PROMOTER REGIONS (GENETICS); RECOMBINANT FUSION PROTEINS; TRANSCRIPTION FACTORS;

ANOXYBACILLUS; ANOXYBACILLUS FLAVITHERMUS; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; POSIBACTERIA;

EID: 10644275363     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2004.09.012     Document Type: Article

References (20)

1. G. Georgiou, and P. Valax Expression of correctly folded proteins in Escherichia coli Curr. Opin. Biotechnol. 7 1996 190 197
2. E. De Bernardez Clark Refolding of recombinant proteins Curr. Opin. Biotechnol. 9 1998 157 163
3. J. Hellman, P. Lassila, and P. Maentsaelae In vitro refolding of cyclomaltodextrin glucanotransferase from cytoplasmic inclusion bodies formed upon expression in Escherichia coli Protein Expr. Purif. 6 1995 56 62
4. R. Martins, O. Delgado, and R. Hatti-Kaul Sequence analysis of cyclodextrin glycosyltransferase from the alkaliphilic Bacillus agaradhaerens Biotechnol. Lett. 25 2003 1555 1562
5. J.G. Thomas, and F. Baneyx Protein misfolding and inclusion body formation in recombinant Escherichia coli cells overexpressing heat-shock proteins J. Biol. Chem. 271 1996 11141 11147
6. J.A. Vasina, and F. Baneyx Expression of aggregation-prone recombinant proteins at low temperatures: a comparative study of the Escherichia coli cspA and tac promoter systems Protein Expr. Purif. 9 1997 211 218
7. J. Yin, J.-h. Lin, W.-t. Li, and D.I.C. Wang Evaluation of different promoters and host strains for the high-level expression of collagen-like polymer in Escherichia coli J. Biotechnol. 100 2003 181 191
8. Y.-P. Chao, C.-J. Chiang, and W.-B. Hung Stringent regulation and high-level expression of heterologous genes in Escherichia coli using T7 system controllable by the araBAD promoter Biotechnol. Prog. 18 2002 394 400
9. J.R. Newman, and C. Fuqua Broad-host-range expression vectors that carry the l-arabinose-inducible Escherichia coli araBAD promoter and the araC regulator Gene 227 1999 197 203
10. P.J. Brixey, C. Guda, and H. Daniell The chloroplast psbA promoter is more efficient in Escherichia coli than the T7 promoter for hyperexpression of a foreign protein Biotechnol. Lett. 19 1997 395 399
11. R.B. Kapust, and D.S. Waugh Escherichia coli maltose-binding protein is uncommonly effective at promoting the solubility of polypeptides to which it is fused Protein Sci. 8 1999 1668 1674
12. E.R. La Vallie, Z. Lu, E.A. Diblasio-Smith, L.A. Collins-Racie, and J.M. McCoy Thioredoxin as a fusion partner for production of soluble recombinant proteins in Escherichia coli Methods Enzymol. 326 2000 322 340
13. Y. Zhang, D.R. Olsen, K.B. Nguyen, P.S. Olson, E.T. Rhodes, and D. Mascarenhas Expression of eukaryotic proteins in soluble form in Escherichia coli Protein Expr. Purif. 12 1998 159 165
14. P. Forrer, and R. Jaussi High-level expression of soluble heterologous proteins in the cytoplasm of Escherichia coli by fusion to the bacteriophage lambda head protein D Gene 224 1998 45 52
15. G.D. Davis, C. Elisee, D.M. Newham, and R.G. Harrison New fusion protein systems designed to give soluble expression in Escherichia coli Biotechnol. Bioeng. 65 1999 382 388
16. G.L. Miller Use of dinitrosalicylic acid reagent for determination of reducing sugar Anal. Chem. 31 1959 426 428
17. U. Laemmli Cleavage of structural proteins during assembly of the head of bacteriophage T4 Nature 227 1970 680 685
18. K.H. Park The multisubstrate specificity and the quaternary structure of cyclodextrin-/pullulan-degrading enzymes J. Appl. Glycosci. 48 2001 293 299
19. D.L. Wilkinson, and R.G. Harrison Predicting the solubility of recombinant proteins in Escherichia coli Bio/Technology 9 1991 443 448
20. M. Hammarström, N. Hellgren, S. Van Den Berg, H. Berglund, and T. Härd Rapid screening for improved solubility of small human proteins produced as fusion proteins in Escherichia coli Protein Sci. 11 2002 313 321