-
1
-
-
0025085655
-
Ligation-independent cloning of PCR products (LIC-PCR)
-
Aslanidis C, de Jong PJ: Ligation-independent cloning of PCR products (LIC-PCR). Nucleic Acids Res 1990, 18(20):6069-6074.
-
(1990)
Nucleic Acids Res
, vol.18
, Issue.20
, pp. 6069-6074
-
-
Aslanidis, C.1
de Jong, P.J.2
-
2
-
-
0033676098
-
DNA cloning using in vitro site-specific recombination
-
Hartley JL, Temple GF, Brasch MA: DNA cloning using in vitro site-specific recombination. Genome Res 2000, 10(11):1788-1795.
-
(2000)
Genome Res
, vol.10
, Issue.11
, pp. 1788-1795
-
-
Hartley, J.L.1
Temple, G.F.2
Brasch, M.A.3
-
3
-
-
14844294424
-
Protein production by auto-induction in high-density shaking cultures
-
Studier FW: Protein production by auto-induction in high-density shaking cultures. Protein Expr Purif 2005, 41(1):207-234.
-
(2005)
Protein Expr Purif
, vol.41
, Issue.1
, pp. 207-234
-
-
Studier, F.W.1
-
4
-
-
0034665455
-
Design of high-throughput methods of protein production for structural biology
-
Stevens RC: Design of high-throughput methods of protein production for structural biology. Structure Fold Des 2000, 8(9):R177-85.
-
(2000)
Structure Fold Des
, vol.8
, Issue.9
-
-
Stevens, R.C.1
-
5
-
-
20844452554
-
The matrix refolded
-
Buckle AM, Devlin GL, Jodun RA, Fulton KF, Faux N, Whisstock JC, Bottomley SP: The matrix refolded. Nat Methods 2005, 2(1):3.
-
(2005)
Nat Methods
, vol.2
, Issue.1
, pp. 3
-
-
Buckle, A.M.1
Devlin, G.L.2
Jodun, R.A.3
Fulton, K.F.4
Faux, N.5
Whisstock, J.C.6
Bottomley, S.P.7
-
6
-
-
7044228084
-
Protein expression and refolding - A practical guide to getting the most out of inclusion bodies
-
Cabrita LD, Bottomley SP: Protein expression and refolding - A practical guide to getting the most out of inclusion bodies. Biotechnol Annu Rev 2004, 10:31-50.
-
(2004)
Biotechnol Annu Rev
, vol.10
, pp. 31-50
-
-
Cabrita, L.D.1
Bottomley, S.P.2
-
7
-
-
13844271129
-
From gene to protein: A review of new and enabling technologies for multi-parallel protein expression
-
Hunt I: From gene to protein: a review of new and enabling technologies for multi-parallel protein expression. Protein Expr Purif 2005, 40(1):1-22.
-
(2005)
Protein Expr Purif
, vol.40
, Issue.1
, pp. 1-22
-
-
Hunt, I.1
-
8
-
-
0023680201
-
Vectors that facilitate the expression and purification of foreign peptides in Escherichia coli by fusion to maltose-binding protein
-
di Guan C, Li P, Riggs PD, Inouye H: Vectors that facilitate the expression and purification of foreign peptides in Escherichia coli by fusion to maltose-binding protein. Gene 1988, 67(1):21-30.
-
(1988)
Gene
, vol.67
, Issue.1
, pp. 21-30
-
-
di Guan, C.1
Li, P.2
Riggs, P.D.3
Inouye, H.4
-
9
-
-
4444378435
-
The solubility and stability of recombinant proteins are increased by their fusion to NusA
-
De Marco V, Stier G, Blandin S, de Marco A: The solubility and stability of recombinant proteins are increased by their fusion to NusA. Biochem Biophys Res Commun 2004, 322(3):766-771.
-
(2004)
Biochem Biophys Res Commun
, vol.322
, Issue.3
, pp. 766-771
-
-
De Marco, V.1
Stier, G.2
Blandin, S.3
de Marco, A.4
-
10
-
-
0023806075
-
Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione Stransferase
-
Smith DB, Johnson KS: Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione Stransferase. Gene 1988, 67(1):31-40.
-
(1988)
Gene
, vol.67
, Issue.1
, pp. 31-40
-
-
Smith, D.B.1
Johnson, K.S.2
-
11
-
-
0037184981
-
Structural basis for the substrate specificity of tobacco etch virus protease
-
Phan J, Zdanov A, Evdokimov AG, Tropea JE, Peters HK, Kapust RB, Li M, Wlodawer A, Waugh DS: Structural basis for the substrate specificity of tobacco etch virus protease. J Biol Chem 2002, 277(52):50564-50572.
-
(2002)
J Biol Chem
, vol.277
, Issue.52
, pp. 50564-50572
-
-
Phan, J.1
Zdanov, A.2
Evdokimov, A.G.3
Tropea, J.E.4
Peters, H.K.5
Kapust, R.B.6
Li, M.7
Wlodawer, A.8
Waugh, D.S.9
-
12
-
-
29244442950
-
Simplified screening for the detection of soluble fusion constructs expressed in E. coli using a modular set of vectors
-
Dummler A, Lawrence AM, de Marco A: Simplified screening for the detection of soluble fusion constructs expressed in E. coli using a modular set of vectors. Microb Cell Fact 2005, 4:34.
-
(2005)
Microb Cell Fact
, vol.4
, pp. 34
-
-
Dummler, A.1
Lawrence, A.M.2
de Marco, A.3
-
13
-
-
14344264295
-
Large-scale protein expression for proteome research
-
Korf U, Kohl T, van der Zandt H, Zahn R, Schleeger S, Ueberle B, Wandschneider S, Bechtel S, Schnolzer M, Ottleben H, Wiemann S, Poustka A: Large-scale protein expression for proteome research. Proteomics 2005, 5(14):3571-3580.
-
(2005)
Proteomics
, vol.5
, Issue.14
, pp. 3571-3580
-
-
Korf, U.1
Kohl, T.2
van der Zandt, H.3
Zahn, R.4
Schleeger, S.5
Ueberle, B.6
Wandschneider, S.7
Bechtel, S.8
Schnolzer, M.9
Ottleben, H.10
Wiemann, S.11
Poustka, A.12
-
14
-
-
0036926615
-
A new vector for high-throughput, ligation-independent cloning encoding a tobacco etch virus protease cleavage site
-
Stols L, Gu M, Dieckman L, Raffen R, Collart FR, Donnelly MI: A new vector for high-throughput, ligation-independent cloning encoding a tobacco etch virus protease cleavage site. Protein Expr Purif 2002, 25(1):8-15.
-
(2002)
Protein Expr Purif
, vol.25
, Issue.1
, pp. 8-15
-
-
Stols, L.1
Gu, M.2
Dieckman, L.3
Raffen, R.4
Collart, F.R.5
Donnelly, M.I.6
-
15
-
-
3543050105
-
His tag effect on solubility of human proteins produced in Escherichia coli: A comparison between four expression vectors
-
Woestenenk EA, Hammarstrom M, van den Berg S, Hard T, Berglund H: His tag effect on solubility of human proteins produced in Escherichia coli: a comparison between four expression vectors. J Struct Funct Genomics 2004, 5(3):217-229.
-
(2004)
J Struct Funct Genomics
, vol.5
, Issue.3
, pp. 217-229
-
-
Woestenenk, E.A.1
Hammarstrom, M.2
van den Berg, S.3
Hard, T.4
Berglund, H.5
-
16
-
-
0028566651
-
Minimal length requirement of the single-stranded tails for ligation-independent cloning (LIC) of PCR products
-
Aslanidis C, de Jong PJ, Schmitz G: Minimal length requirement of the single-stranded tails for ligation-independent cloning (LIC) of PCR products. PCR Methods Appl 1994, 4(3):172-177.
-
(1994)
PCR Methods Appl
, vol.4
, Issue.3
, pp. 172-177
-
-
Aslanidis, C.1
de Jong, P.J.2
Schmitz, G.3
-
17
-
-
0032439236
-
Protein engineering of chimeric Serpins: An investigation into effects of the serpin scaffold and reactive centre loop length
-
Bottomley SP, Stone SR: Protein engineering of chimeric Serpins: an investigation into effects of the serpin scaffold and reactive centre loop length. Protein Eng 1998, 11(12):1243-1247.
-
(1998)
Protein Eng
, vol.11
, Issue.12
, pp. 1243-1247
-
-
Bottomley, S.P.1
Stone, S.R.2
-
18
-
-
0347753711
-
Production of recombinant serpins in Escherichia coli
-
Bird PI, Pak SC, Worrall DM, Bottomley SP: Production of recombinant serpins in Escherichia coli. Methods 2004, 32(2):169-176.
-
(2004)
Methods
, vol.32
, Issue.2
, pp. 169-176
-
-
Bird, P.I.1
Pak, S.C.2
Worrall, D.M.3
Bottomley, S.P.4
-
19
-
-
3142608984
-
Joint X-ray and NMR refinement of the yeast L30e-mRNA complex
-
Chao JA, Williamson JR: Joint X-ray and NMR refinement of the yeast L30e-mRNA complex. Structure 2004, 12(7):1165-1176.
-
(2004)
Structure
, vol.12
, Issue.7
, pp. 1165-1176
-
-
Chao, J.A.1
Williamson, J.R.2
-
20
-
-
0037305746
-
Insights into binding cooperativity of MATal/MATalpha2 from the crystal structure of a MATal homeodomain-maltose binding protein chimera
-
Ke A, Wolberger C: Insights into binding cooperativity of MATal/ MATalpha2 from the crystal structure of a MATal homeodomain-maltose binding protein chimera. Protein Sci 2003, 12(2):306-312.
-
(2003)
Protein Sci
, vol.12
, Issue.2
, pp. 306-312
-
-
Ke, A.1
Wolberger, C.2
-
21
-
-
0038648648
-
Crystal structures of fusion proteins with large-affinity tags
-
Smyth DR, Mrozkiewicz MK, McGrath WJ, Listwan P, Kobe B: Crystal structures of fusion proteins with large-affinity tags. Protein Sci 2003, 12(7):1313-1322.
-
(2003)
Protein Sci
, vol.12
, Issue.7
, pp. 1313-1322
-
-
Smyth, D.R.1
Mrozkiewicz, M.K.2
McGrath, W.J.3
Listwan, P.4
Kobe, B.5
-
22
-
-
0036006255
-
Differential effects of short affinity tags on the crystallization of Pyrococcus furiosus maltodextrin-binding protein
-
Bucher MH, Evdokimov AG, Waugh DS: Differential effects of short affinity tags on the crystallization of Pyrococcus furiosus maltodextrin-binding protein. Acta Crystallogr D Biol Crystallogr 2002, 58(Pt 3):392-397.
-
(2002)
Acta Crystallogr D Biol Crystallogr
, vol.58
, Issue.PART 3
, pp. 392-397
-
-
Bucher, M.H.1
Evdokimov, A.G.2
Waugh, D.S.3
|