Production of recombinant proteins in Escherichia coli using an N-terminal tag derived from sortase

Protein Expression and Purification

Volumn 70, Issue 2, 2010, Pages 143-150

  Caswell, Jill ^a,b   Snoddy, Philip ^a   McMeel, Darragh ^a   Buick, Richard J ^a   Scott, Christopher J ^b  

^a FUSION ANTIBODIES LTD   (United Kingdom)

^b QUEEN'S UNIVERSITY BELFAST   (United Kingdom)

Author keywords

Fusion tag; Protein expression; Solubility; Sortase

Indexed keywords

AMINOACYLTRANSFERASE; BACTERIAL PROTEIN; CYSTEINE PROTEINASE; DIFFERENTIATION ANTIGEN; ESCHERICHIA COLI PROTEIN; HYBRID PROTEIN; LECTIN; LEUKOCYTE ANTIGEN; RECOMBINANT PROTEIN; SIGLEC5 PROTEIN, HUMAN; SORTASE A;

AMINO ACID SEQUENCE; ARTICLE; BIOSYNTHESIS; BIOTECHNOLOGY; ESCHERICHIA COLI; GENE VECTOR; GENETICS; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; MOLECULAR CLONING; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PROTEIN DENATURATION; WESTERN BLOTTING;

AMINO ACID SEQUENCE; AMINOACYLTRANSFERASES; ANTIGENS, CD; ANTIGENS, DIFFERENTIATION, MYELOMONOCYTIC; BACTERIAL PROTEINS; BASE SEQUENCE; BIOTECHNOLOGY; BLOTTING, WESTERN; CLONING, MOLECULAR; CYSTEINE ENDOPEPTIDASES; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENETIC VECTORS; HUMANS; LECTINS; MOLECULAR SEQUENCE DATA; PROTEIN DENATURATION; RECOMBINANT FUSION PROTEINS; RECOMBINANT PROTEINS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 76749157163     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2009.10.012     Document Type: Article

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