The Escherichia coli small heat-shock proteins IbpA and IbpB prevent the aggregation of endogenous proteins denatured in vivo during extreme heat shock

Microbiology

Volumn 148, Issue 6, 2002, Pages 1757-1765

  Kuczyńska Wiśnik, Dorota ^a   Kȩdzierska, Sabina ^a   Matuszewska, Ewelina ^a   Lund, Peter ^a   Taylor, Alina ^a   Lipińska, Barbara ^a   Laskowska, Ewa ^a  

^a UNIVERSITY OF GDA SK   (Poland)

Author keywords

DnaK; Protein aggregation; lactamase precursor

Indexed keywords

BETA LACTAMASE; CHAPERONE; ENZYME PRECURSOR; FRUCTOSE BISPHOSPHATE ALDOLASE; GLUCOSE; HEAT SHOCK PROTEIN; PROTEIN LBPA; PROTEIN LBPB; UNCLASSIFIED DRUG; DNAK PROTEIN, E COLI; ESCHERICHIA COLI PROTEIN; HEAT SHOCK PROTEIN 70; IBPA PROTEIN, E COLI; IBPB PROTEIN, E COLI;

ARTICLE; CELL VIABILITY; CENTRIFUGATION; CONTROLLED STUDY; ENZYME INACTIVATION; ENZYME RENATURATION; ESCHERICHIA COLI; GENE DELETION; HEAT SHOCK; IN VITRO STUDY; IN VIVO STUDY; INCUBATION TIME; NONHUMAN; OPERON; PRIORITY JOURNAL; PROTEIN AGGREGATION; PROTEIN BINDING; PROTEIN DENATURATION; PROTEIN FOLDING; PROTEIN ISOLATION; PROTEIN LOCALIZATION; PROTEIN STABILITY; TEMPERATURE; WILD TYPE; BIOLOGICAL MODEL; CHEMISTRY; GENE EXPRESSION; GENETICS; HEAT SHOCK RESPONSE; METABOLISM; PHYSIOLOGY;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA;

BETA-LACTAMASES; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENE DELETION; GENE EXPRESSION; HEAT-SHOCK PROTEINS; HEAT-SHOCK RESPONSE; HSP70 HEAT-SHOCK PROTEINS; MODELS, GENETIC; MOLECULAR CHAPERONES; OPERON; PROTEIN DENATURATION;

EID: 0035987265     PISSN: 13500872     EISSN: None     Source Type: Journal    
DOI: 10.1099/00221287-148-6-1757     Document Type: Article

References (27)

1. Two novel heat shock genes encoding proteins produced in response to heterologous protein expression in E. coli
2. A rapid and sensitive method for quantition of protein utilizing the principle of protein-dye binding
3. Characterization of alpha-crystallin-plasma membrane binding
4. Binding of non-native protein to Hsp25 during heat shock creates a reservoir of folding intermediates for reactivation
5. Assisting spontaneity: The role of Hsp90 and small Hsp as molecular chaperones
6. The role of DnaK/DnaJ and GroEL/GroES systems in the removal of endogenous proteins aggregated by heat-shock from Escherichia coli cells
7. DnaK/DnaJ chaperone system reactivates endogenous E. coli thermostable FBP aldolase in vivo and in vitro; the effect is enhanced by GroE heat shock proteins
8. Small heat shock proteins, IbpA and IbpB, are involved in resistances to heat and superoxide stresses in E. coli
9. Response of E. coli cell membranes to induction of λcI857 prophage by heat shock
10. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
11. The Escherichia coli heat shock proteins GroEL and GroES modulate the folding of the beta-lactamase precursor
12. IbpA and IbpB, the new heat-shock proteins, bind to endogenous Escherichia coli proteins aggregated intracellularly by heat shock
13. Degradation by proteases Lon, Clp and HtrA of Escherichia coli proteins aggregated in vivo by heat shock; HtrA protease action in vivo and in vitro
14. A small heat shock protein cooperates with heat shock protein 70 system to reactivate a heat-denatured protein
15. Identification of a site of Hsp27 binding with Hsp27 and αB-crystallin as indicated by the yeast two-hybrid system
16. Structure and function of small heat shock α crystalline proteins: Established concept and emerging ideas
17. Construction and characterization of a set of E. coli strains deficient in all known loci affecting the proteolytic stability of secreted recombinant proteins
18. Identification of thermolabile E. coli proteins: Prevention and reversion of aggregation by DnaK and ClpB
19. Heat shock response
20. Interaction of human recombinant αA- and αB-crystallins with early and late unfolding intermediates of citrate synthase on its thermal denaturation
21. Biochemical characterization of the small heat shock protein IbpB from Escherichia coli
22. Chaperone activity and homo-and hetero-oligomer formation of bacterial small heat shock proteins
23. Roles of the E. coli small heat shock proteins IbpA and IbpB in thermal stress management: Comparison with ClpA, ClpB and HtpG in vivo
24. Levels of DnaK and DnaJ provide tight control of heat shock gene expression and protein repair in E. coli
25. The small heat-shock protein IbpB from E. coli stabilizes stress-denatured proteins for subsequent refolding by a multichaperone network
26. Involvement of the DnaK-DnaJ-GrpE chaperone team in protein secretion in Escherichia coli
27. Protein and peptide purification