Improved isolation of proteins tagged with glutathione S-transferase

Protein Expression and Purification

Volumn 75, Issue 2, 2011, Pages 161-164

  Vinckier, Nicholas K ^a   Chworos, Arkadiusz ^a   Parsons, Stanley M ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Fusion protein; Gamma hydroxybutyrate dehydrogenase; GHB; Glutathione; Glutathione S transferase; S butylglutathione

Indexed keywords

DRUG DERIVATIVE; GLUTATHIONE; GLUTATHIONE TRANSFERASE; HYBRID PROTEIN;

AFFINITY CHROMATOGRAPHY; ANIMAL; ARTICLE; CHEMISTRY; DIMERIZATION; ESCHERICHIA COLI; GEL CHROMATOGRAPHY; GENETICS; ISOLATION AND PURIFICATION; LIGHT; METHODOLOGY; POLYMERIZATION; RADIATION SCATTERING; SCHISTOSOMA JAPONICUM;

ANIMALS; CHROMATOGRAPHY, AFFINITY; CHROMATOGRAPHY, GEL; DIMERIZATION; ESCHERICHIA COLI; GLUTATHIONE; GLUTATHIONE TRANSFERASE; LIGHT; POLYMERIZATION; RECOMBINANT FUSION PROTEINS; SCATTERING, RADIATION; SCHISTOSOMA JAPONICUM;

EID: 78649332548     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2010.09.006     Document Type: Article

References (26)

1. J. Paulsson, and G. Westermark Studies of the amyloidogenic properties of proIAPP, amyloid and amyloidosis 2005 CRC Press pp. 446-448
2. X. Gao, J. Zhao, L. Fan, S. Li, F. Wang, S. Ji, and Q. Yuan Purification and properties of recombinant GST-haparinase III and optimization of cultivation conditions Sheng Wu Gong Cheng Xue Bao 25 2009 1718 1724
3. A.-M. Krogsdam, K. Kristiansen, and J. Nøhr Expression in E. coli Systems Method Mol. Biol. 232 2003 103 109
4. D.T. Bravo, D.O. Harris, and S.M. Parsons Reliable, sensitive, rapid and quantitative enzyme-based assay for gamma-hydroxybutyric acid (GHB) J. Forensic. Sci. 49 2004 379 387
5. K. Lim, J.X. Ho, K. Keeling, G.L. Gilliland, X. Ji, F. Ruker, and D.C. Carter Three-dimensional structure of Schistosoma japonicum glutathione S-transferase fused with a six-amino acid conserved neutralizing epitope of gp41 from HIV Protein Sci. 3 1994 2233 2244
6. J.B. Wheatley, B. Hughes, K. Bauer, and D.E. Schmidt Jr Study of chromatographic parameters for glutathione S transferases on an high-performance liquid chromatography affinity stationary phase J. Chromatogr. A 676 1994 81 90
7. M.A. McTigue, D.R. Williams, and J.A. Tainer Crystal structures of a schistosomal drug and vaccine target: glutathione S-transferase from Schistosoma japonica and its complex with the leading antischistosomal drug praziquantel J. Mol. Biol. 246 1995 21 27
8. W. Kaplan, P. Husler, H. Klump, J. Erhardt, N. Sluis-Cremer, and H. Dirr Conformational stability of pGEX-expressed Schistosoma japonicum glutathione S-transferase: a detoxification enzyme and fusion-protein affinity tag Protein Sci 6 1997 399 406
9. D.S. Waugh Making the most of affinity tags Trends Biotechnol. 23 2005 316 320
10. BIOSCI. Available from: < http://www.bio.net/bionet/mm/methods/1994- November/020946.html > (accessed 13.7.2010).
11. Protocol Online. Available from: < http://www.protocol-online.org/ biology-forums/posts/32627.html > (accessed 13.7.2010).
12. N.H. Tolia, and L. Joshua-Tor Strategies for protein coexpression in Escherichia coli Nat. Meth. 3 2006 55 64
13. H.E. Valentin, G. Zwingmann, A. Schonebaum, and A. Steinbuchel Metabolic pathway for biosynthesis of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) from 4-hydroxybutyrate by Alcaligenes eutrophus Eur. J. Biochem. 227 1995 43 60
14. T. Conway, G.W. Sewell, Y.A. Osman, and L.O. Ingram Cloning and sequencing of the alcohol dehydrogenase II gene from Zymomonas mobilis J. Bacteriol. 169 1987 2591 2597
15. R.A. Wolff, and W.R. Kenealy Purification and characterization of the oxygen-sensitive 4-hydroxybutanoate dehydrogenase from Clostridium kluyveri Protein Expr. Purif. 6 1994 206 212
16. K. Ma, and M.W. Adams An unusual oxygen-sensitive, iron- and zinc-containing alcohol dehydrogenase from the hyperthermophilic archaeon Pyrococcus furiosus J. Bacteriol. 181 1999 1163 1170
17. C. Montella, L. Bellsolell, R. Perez-Luque, J. Badia, L. Baldoma, M. Coll, and J. Aguilar Crystal structure of an iron-dependent group III dehydrogenase that interconverts l-lactaldehyde and l-1, 2-propanediol in Escherichia coli J. Bacteriol. 187 2005 4957 4966
18. X. Ying, A.M. Grunden, L. Nie, M.W. Adams, and K. Ma Molecular characterization of the recombinant iron-containing alcohol dehydrogenase from the hyperthermophilic Archaeon, Thermococcus strain ES1 Extremophiles 13 2008 299 311
19. D. Marcal, A.T. Rego, M.A. Carrondo, and F.J. Enguita 1,3-Propanediol dehydrogenase from Klebsiella pneumoniae: decameric quaternary structure and possible subunit cooperativity J. Bacteriol. 191 2009 1143 1151
20. F. Yan, W.K. Yang, X.Y. Li, T.T. Lin, Y.N. Lun, F. Lin, S.W. Lv, G.L. Yan, J.Q. Liu, J.C. Shen, Y. Mu, and G.M. Luo A trifunctional enzyme with glutathione S-transferase, glutathione peroxidase and superoxide dismutase activity Biochim. Biophys. Acta 1780 2008 869 872
21. J.D. Rowe, Y.V. Patskovsky, L.N. Patskovska, E. Novikova, and I. Listowsky Rationale for reclassification of a distinctive subdivision of mammalian class Mu glutathione S-transferases that are primarily expressed in testis J. Biol. Chem. 273 1998 9593 9601
22. E. Ortiz-Salmeron, Z. Yassin, M.J. Clemente-Jimenez, F.J. Las Heras-Vazquez, F. Rodriguez-Vico, C. Baron, and L. Garcia-Fuentes A calorimetric study of the binding of S-alkylglutathiones to glutathione S-transferase Biochim. Biophys. Acta 1548 2001 106 113
23. P.J. Wyatt Light scattering and the absolute characterization of macromolecules Anal. Chim. Acta 272 1993 1 40
24. J. Wen, T. Arakawa, and J.S. Philo Size-exclusion chromatography with on-line light-scattering, absorbance, and refractive index detectors for studying proteins and their interactions Anal. Biochem. 240 1996 155 166
25. M. Andujar-Sanchez, A.W. Smith, J.M. Clemente-Jimenez, F. Rodriguez-Vico, F.J. Las Heras-Vazquez, V. Jara-Perez, and A. Camara-Artigas Crystallographic and thermodynamic analysis of the binding of S-octylglutathione to the Tyr 7 to Phe mutant of glutathione S-transferase from Schistosoma japonicum Biochemistry 44 2005 1174 1183
26. R.E. Hansen, D. Roth, and J.R. Winther Quantifying the global cellular thiol-disulfide status Proc. Natl. Acad. Sci. USA 106 2009 422 427