Protein aggregation during overexpression limited by peptide extensions with large net negative charge

Protein Expression and Purification

Volumn 36, Issue 2, 2004, Pages 207-216

  Zhang, Yian Biao ^a   Howitt, Jason ^a,b   McCorkle, Sean ^a   Lawrence, Paul ^a   Springer, Karen ^a   Freimuth, Paul ^a  

^a BROOKHAVEN NATIONAL LABORATORY   (United States)

^b IMPERIAL COLLEGE LONDON   (United Kingdom)

Author keywords

Aggregation; Electrostatic repulsion; Fusion protein; Protein folding

Indexed keywords

AMINO ACID; CAR RECEPTOR; GPA33 PROTEIN, HUMAN; IMMUNOGLOBULIN G; MEMBRANE PROTEIN; RECOMBINANT PROTEIN; VIRUS RECEPTOR;

ARTICLE; BIOSYNTHESIS; CHEMISTRY; CYTOPLASM; ELECTRICITY; ESCHERICHIA COLI; GENE EXPRESSION; GENETICS; HUMAN; IMMUNOGLOBULIN VARIABLE REGION; IMMUNOLOGY; MYELIN SHEATH; PROTEIN FOLDING; PROTEIN TERTIARY STRUCTURE; STRUCTURAL HOMOLOGY;

AMINO ACIDS; CYTOPLASM; ELECTROSTATICS; ESCHERICHIA COLI; GENE EXPRESSION; HUMANS; IMMUNOGLOBULIN G; IMMUNOGLOBULIN VARIABLE REGION; MEMBRANE GLYCOPROTEINS; MYELIN SHEATH; PROTEIN FOLDING; PROTEIN STRUCTURE, TERTIARY; RECEPTORS, VIRUS; RECOMBINANT PROTEINS; STRUCTURAL HOMOLOGY, PROTEIN;

ADENOVIRIDAE; COXSACKIEVIRUS; ESCHERICHIA COLI;

EID: 3142609724     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2004.04.020     Document Type: Article

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