Hyper-acidic protein fusion partners improve solubility and assist correct folding of recombinant proteins expressed in Escherichia coli

Journal of Biotechnology

Volumn 135, Issue 4, 2008, Pages 333-339

  Zou, Zhurong ^a   Cao, Lijuan ^a   Zhou, Pei ^a   Su, Yu ^a   Sun, Yonghong ^a   Li, Wenjuan ^a  

^a EAST CHINA NORMAL UNIVERSITY   (China)

Author keywords

Hyper acidic protein fusion partner; Intramolecular chaperone; Msyb; Rpod; Soluble protein expression; Yjgd

Indexed keywords

AGRICULTURAL PRODUCTS; BIOMOLECULES; ESCHERICHIA COLI; FUSION REACTIONS; NUCLEAR PHYSICS; NUCLEIC ACIDS; PROTEINS; RNA; SOLUBILITY; TARGETS; TERNARY SYSTEMS; TOBACCO; WATER POLLUTION;

ACIDIC PROTEINS; BIS(2 ,4 DINITROPHENYL) PHOSPHATE; E. COLI; ELSEVIER (CO); FUSION PARTNERS; FUSION TAGS; IN-VITRO; IN-VIVO; INTRAMOLECULAR CHAPERONES; N TERMINAL DOMAIN (NTD); PROTEIN AGGREGATION; RECOMBINANT PROTEINS; RIBULOSE; RNA POLYMERASE (RNAP); SOLUBLE EXPRESSION; TARGET PROTEINS; TOBACCO ETCH VIRUS (TEV);

PROTEIN FOLDING;

ACID PROTEIN; CHAPERONE; ENTEROPEPTIDASE; HYBRID PROTEIN; POLYPEPTIDE; RECOMBINANT PROTEIN; RNA POLYMERASE;

ARTICLE; ESCHERICHIA COLI; IN VITRO STUDY; IN VIVO STUDY; NONHUMAN; PRIORITY JOURNAL; PROTEIN AGGREGATION; PROTEIN EXPRESSION; PROTEIN TARGETING;

CYSTEINE ENDOPEPTIDASES; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENDOPEPTIDASES; ESCHERICHIA COLI; GENETIC VECTORS; MOLECULAR CHAPERONES; PROTEIN FOLDING; PROTEIN STRUCTURE, QUATERNARY; RECOMBINANT FUSION PROTEINS; SACCHAROMYCES CEREVISIAE; SMALL UBIQUITIN-RELATED MODIFIER PROTEINS; SOLUBILITY;

BOVINAE; ESCHERICHIA COLI; NICOTIANA TABACUM; TOBACCO ETCH VIRUS;

EID: 47249104180     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2008.05.007     Document Type: Article

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