Recombinant protein expression and purification: A comprehensive review of affinity tags and microbial applications

Biotechnology Journal

Volumn 7, Issue 5, 2012, Pages 620-634

  Young, Carissa L ^a   Britton, Zachary T ^a,b   Robinson, Anne S ^a  

^a University of Delaware   (United States)

^b Tulane University   (United States)

Author keywords

Affinity purification; Affinity tags; Proteases; Solubility enhancement

Indexed keywords

AFFINITY PURIFICATION; AFFINITY TAGS; DRUG DISCOVERY; PRODUCT YIELDS; PROTEASES; PROTEIN DETECTION; PROTEIN FUSION; PROTEIN PURIFICATION; PROTEIN STRUCTURES; RECOMBINANT PROTEIN EXPRESSION; SOLUBILITY ENHANCEMENT;

GENETIC ENGINEERING; RECOMBINANT PROTEINS; SOLUBILITY;

PURIFICATION;

BLOOD CLOTTING FACTOR 10A; ENTEROPEPTIDASE; EPITOPE; GLUTATHIONE TRANSFERASE; HEMAGGLUTININ; MALTOSE BINDING PROTEIN; MEMBRANE PROTEIN; MISTIC PROTEIN; MYC PROTEIN; N UTILIZATION SUBSTANCE A PROTEIN; POLYARGININE; PROTEIN TAG; STEROID DELTA ISOMERASE; STREPTAVIDIN; SUMO PROTEIN; THIOREDOXIN; THIOREDOXIN A; THROMBIN; TOBACCO ETCH VIRUS PROTEINASE; TRANSCRIPTION FACTOR; UNCLASSIFIED DRUG; VIRUS PROTEIN;

BINDING AFFINITY; BIOTECHNOLOGY; DRUG DEVELOPMENT; ESCHERICHIA COLI; GENE PRODUCT; HUMAN; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN PURIFICATION; PROTEIN STRUCTURE; REVIEW; SOLUBILITY;

ANIMALS; BIOTECHNOLOGY; CHROMATOGRAPHY, AFFINITY; HUMANS; PEPTIDE HYDROLASES; PROTEIN ENGINEERING; RECOMBINANT FUSION PROTEINS; SOLUBILITY;

EID: 84860309632     PISSN: 18606768     EISSN: 18607314     Source Type: Journal    
DOI: 10.1002/biot.201100155     Document Type: Review

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