Comparison of SUMO fusion technology with traditional gene fusion systems: Enhanced expression and solubility with SUMO

Protein Science

Volumn 15, Issue 1, 2006, Pages 182-189

  Marblestone, Jeffrey G ^a   Edavettal, Suzanne C ^b   Lim, Yiting ^b   Lim, Peter ^a   Zuo, Xun ^b   Butt, Tauseef R ^b  

^a Progenra Inc   (United States)

^b LifeSensors Inc   (United States)

Author keywords

Fusion protein; Protease; Structural genomics; SUMO; Ubiquitin like protein

Indexed keywords

COLLAGENASE 3; GLUTATHIONE TRANSFERASE; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; MALTOSE BINDING PROTEIN; MYOSTATIN; PROTEIN; PROTEIN NUS A; PROTEINASE; RECOMBINANT PROTEIN; SUMO PROTEIN; THIOREDOXIN; UBIQUITIN; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; CATALYSIS; CHEMICAL REACTION KINETICS; ENZYME ACTIVITY; ENZYME KINETICS; ESCHERICHIA COLI; EXPRESSION VECTOR; GENE CONSTRUCT; GENE FUSION; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN DEGRADATION; PROTEIN ENGINEERING; PROTEIN EXPRESSION; PROTEIN PROTEIN INTERACTION; PROTEIN SYNTHESIS; RECOMBINANT DNA TECHNOLOGY;

CLONING, MOLECULAR; CYSTEINE ENDOPEPTIDASES; ENDOPEPTIDASES; ESCHERICHIA COLI; GENE FUSION; HUMANS; RECOMBINANT FUSION PROTEINS; SOLUBILITY; SUMO-1 PROTEIN;

ESCHERICHIA COLI;

EID: 29344475982     PISSN: 09618368     EISSN: 1469896X     Source Type: Journal    
DOI: 10.1110/ps.051812706     Document Type: Article

References (33)

1. Arechaga, I., Miroux, B., Runswick, M.J., and Walker, J.E. 2003. Over-expression of Escherichia coli F1F(o)-ATPase subunit a is inhibited by instability of the uncB gene transcript. FEBS Lett. 547: 97-100.
2. Bayer, P., Arndt, A., Metzger, S., Mahajan, R., Melchior, F., Jaenicke, R., and Becker, J. 1998. Structure determination of the small ubiquitinrelated modifier SUMO-1. J. Mol. Biol. 280: 275-286.
3. Carrington, J.C., Cary, S.M., Parks, T.D., and Dougherty, W.G. 1989. A second proteinase encoded by a plant potyvirus genome. EMBO J. 8: 365-370.
4. Creighton, T.E. 1997. How important is the molten globule for correct protein folding? Trends Biochem. Sci. 22: 6-10.
5. Davis, G.D., Elisee, C., Newham, D.M., and Harrison, R.G. 1999. New fusion protein systems designed to give soluble expression in Escherichia coli. Biotechnol. Bioeng. 65: 382-388.
6. De Marco, V., Stier, G., Blandin, S., and de Marco, A. 2004. The solubility and stability of recombinant proteins are increased by their fusion to NusA. Biochem. Biophys. Res. Commun. 322: 766-771.
7. Englander, S.W. 2000. Protein folding intermediates and pathways studied by hydrogen exchange. Annu. Rev. Biophys. Biomol. Struct. 29: 213-238.
8. Hamilton, S.R., O'Donnell Jr., J.B., Hammet, A., Stapleton, D., Habinowski, S.A., Means, A.R., Kemp, B.E., and Witters, L.A. 2002. AMP-activated protein kinase kinase: Detection with recombinant AMPK α1 subunit. Biochem. Biophys. Res. Commun. 293: 892-898.
9. Hardern, I.M., Knauper, V., Ernill, R.J., Taylor, I.W., Cooper, K.L., and Abbott, W.M. 2000. An analysis of two refolding routes for a C-terminally truncated human collagenase-3 expressed in Escherichia coli. Protein Expr. Purif. 19: 246-252.
10. Ikura, K., Kokubu, T., Natsuka, S., Ichikawa, A., Adachi, M., Nishihara, K., Yanagi, H., and Utsumi, S. 2002. Co-overexpression of folding modulators improves the solubility of the recombinant guinea pig liver transglutaminase in Escherichia coli. Prep. Biochem. Biotechnol. 32: 189-205.
11. Jenny, R.J., Mann, K.G., and Lundblad, R.L. 2003. A critical review of the methods for cleavage of fusion proteins with thrombin and factor Xa. Protein Expr. Purif. 31: 1-11.
12. Johnson, E.S. and Blobel, G. 1999. Cell cycle-regulated attachment of the ubiquitin-related protein SUMO to the yeast septins. J. Cell. Biol. 147: 981-994.
13. Kawabe, Y., Seki, M., Seki, T., Wang, W.S., Imamura, O., Furuichi, Y., Saitoh, H., and Enomoto, T. 2000. Covalent modification of the Werner's syndrome gene product with the ubiquitin-related protein, SUMO-1. J. Biol. Chem. 275: 20963-20966.
14. Khorasanizadeh, S., Peters, I.D., and Roder, H. 1996. Evidence for a three-state model of protein folding from kinetic analysis of ubiquitin variants with altered core residues. Nat. Struct. Biol. 3: 193-205.
15. Lee, S.J. and McPherron, A.C. 2001. Regulation of myostatin activity and muscle growth. Proc. Natl. Acad. Sci. 98: 9306-9311.
16. Li, S.J. and Hochstrasser, M. 1999. A new protease required for cell-cycle progression in yeast. Nature 398: 246-251.
17. _. 2000. The yeast ULP2 (SMT4) gene encodes a novel protease specific for the ubiquitin-like Smt3 protein. Mol. Cell. Biol. 20: 2367-2377.
18. Malakhov, M.P., Mattern, M.R., Malakhova, O.A., Drinker, M., Weeks, S.D., and Butt, T.R. 2004. SUMO fusions and SUMO-specific protease for efficient expression and purification of proteins. J. Struct. Funct. Genomics 5: 75-86.
19. Melchior, F. 2000. SUMO - nonclassical ubiquitin. Annu. Rev. Cell. Dev. Biol. 16: 591-626.
20. Mossessova, E. and Lima, C.D. 2000. Ulp1-SUMO crystal structure and genetic analysis reveal conserved interactions and a regulatory element essential for cell growth in yeast. Mol. Cell 5: 865-876.
21. Muller, S., Matunis, M.J., and Dejean, A. 1998. Conjugation with the ubiquitin-related modifier SUMO-1 regulates the partitioning of PML within the nucleus. EMBO J. 17: 61-70.
22. Nallamsetty, S., Kapust, R.B., Tozser, J., Cherry, S., Tropea, J.E., Copeland, T.D., and Waugh, D.S. 2004. Efficient site-specific processing of fusion proteins by tobacco vein mottling virus protease in vivo and in vitro. Protein Expr. Purif. 38: 108-115.
23. Pathak, N., Hu, S.I., and Koehn, J.A. 1998. The expression, refolding, and purification of the catalytic domain of human collagenase-3 (MMP-13). Protein Expr. Purif. 14: 283-288.
24. Podmore, A.H. and Reynolds, P.E. 2002. Purification and characterization of VanXY(C), a D, D-dipeptidase/D, D-carboxypeptidase in vancomycin-resistant Enterococcus gallinarum BM4174. Eur. J. Biochem. 269: 2740-2746.
25. Pryor, K.D. and Leiting, B. 1997. High-level expression of soluble protein in Escherichia coli using a His6-tag and maltose-binding-protein double-affinity fusion system. Protein Expr. Purif. 10: 309-319.
26. Sachdev, D. and Chirgwin, J.M. 2000. Fusions to maltose-binding protein: Control of folding and solubility in protein purification. Methods Enzymol. 326: 312-321.
27. Studier, F.W. and Moffatt, B.A. 1986. Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. J. Mol. Biol. 189: 113-130.
28. Tatham, M.H., Jaffray, E., Vaughan, O.A., Desterro, J.M., Botting, C.H., Naismith, J.H., and Hay, R.T. 2001. Polymeric chains of SUMO-2 and SUMO-3 are conjugated to protein substrates by SAE1/SAE2 and Ubc9. J. Biol. Chem. 276: 35368-35374.
29. Taylor, W.E., Bhasin, S., Artaza, J., Byhower, F., Azam, M., Willard Jr., D.H., Kull Jr., F.C., and Gonzalez-Cadavid, N. 2001. Myostatin inhibits cell proliferation and protein synthesis in C2C12 muscle cells. Am. J. Physiol. Endocrinol. Metab. 280: E221-E228.
30. Thomas, M., Langley, B., Berry, C., Sharma, M., Kirk, S., Bass, J., and Kambadur, R. 2000. Myostatin, a negative regulator of muscle growth, functions by inhibiting myoblast proliferation. J. Biol. Chem. 275: 40235-40243.
31. Wang, C., Castro, A.F., Wilkes, D.M., and Altenberg, G.A. 1999. Expression and purification of the first nucleotide-binding domain and linker region of human multidrug resistance gene product: Comparison of fusions to glutathione S-transferase, thioredoxin and maltose-binding protein. Biochem. J. 338: 77-81.
32. Zuo, X., Li, S., Hall, J., Mattern, M.R., Tran, H., Shoo, J., Tan, R., Weiss, S.R., and Butt, T.R. 2005a. Enhanced expression and purification of membrane proteins by SUMO fusion in E. coli. J. Struct. Funct. Genomics 6: 103-111.
33. Zuo, X., Mattern, M.R., Tan, R., Li, S., Hall, J., Sterner, D.E., Shoo, J., Tran, H., Lim, P., Sarafianos, S.G., et al. 2005b. Expression and purification of SARS Coronavirus proteins using SUMO fusions. Protein Express Purif. 42: 100-110.