Construction and use of new cloning vectors for the rapid isolation of recombinant proteins from Escherichia coli

Plasmid

Volumn 59, Issue 3, 2008, Pages 231-237

  Rocco, C J ^a   Dennison, K L ^a   Klenchin, Vadim A ^a   Rayment, I ^a   Escalante Semerena, J C ^a  

^a UNIVERSITY OF WISCONSIN MADISON   (United States)

Author keywords

2 Methylaconitate isomerase; 2 Methylcitrate dehydratase; 2 Methylcitric acid cycle enzymes; Propionate catabolism; Rapid protein purification; TEV protease cleavable tags

Indexed keywords

2 METHYLACONITATE ISOMERASE; 2 METHYLCITRATE DEHYDRATASE; BACTERIAL PROTEIN; HEXAHISTIDINE; MALTOSE BINDING PROTEIN; PLASMID VECTOR; PROTEINASE; PYRUVIC ACID; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; CATABOLISM; CLONING VECTOR; CONTROLLED STUDY; ESCHERICHIA COLI; GENE CONSTRUCT; NONHUMAN; PLASMID; PLASMID KLD116; PLASMID KLD66; PLASMID TEV3; PLASMID TEV4; PLASMID TEV5; PROTEIN EXPRESSION; PROTEIN ISOLATION; PROTEIN PURIFICATION; PROTEIN SYNTHESIS; SALMONELLA ENTERICA; SHEWANELLA ONEIDENSIS;

BACTERIAL PROTEINS; CITRATES; CLONING, MOLECULAR; ENDOPEPTIDASES; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENETIC TECHNIQUES; GENETIC VECTORS; HISTIDINE; HYDRO-LYASES; MODELS, GENETIC; OLIGOPEPTIDES; PLASMIDS; RECOMBINANT FUSION PROTEINS; RECOMBINANT PROTEINS; SALMONELLA ENTERICA; SHEWANELLA;

ESCHERICHIA COLI; SALMONELLA ENTERICA; SHEWANELLA ONEIDENSIS; TOBACCO ETCH VIRUS;

EID: 41549104836     PISSN: 0147619X     EISSN: 10959890     Source Type: Journal    
DOI: 10.1016/j.plasmid.2008.01.001     Document Type: Article

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