Construction of a dual-tag system for gene expression, protein affinity purification and fusion protein processing

Biotechnology Letters

Volumn 31, Issue 4, 2009, Pages 543-549

  Motejadded, Hassan ^a   Altenbuchner, Josef ^a  

^a UNIVERSITY OF STUTTGART   (Germany)

Author keywords

Gene expression; Protein affinity purification; Proteolytic cleavage; SUMO protein; Ulp1 protease

Indexed keywords

AFFINITY PURIFICATIONS; CELL EXTRACTS; E. COLI; ENHANCED GREEN FLUORESCENT PROTEINS; FLOW-THROUGH; FUSION GENES; FUSION PROTEINS; HIS TAGS; L RHAMNOSE; MALTOSE-BINDING PROTEINS; PROTEIN AFFINITY PURIFICATION; PROTEIN PROCESSING; PROTEOLYTIC CLEAVAGE; S. CEREVISIAE; SACCHAROMYCES CEREVISIAE; SUMO PROTEIN; TAG SYSTEMS; ULP1 PROTEASE; VECTOR SYSTEMS;

BIOACTIVITY; ESCHERICHIA COLI; GENE EXPRESSION; GLYCOSYLATION; MALTOSE; PROTEINS; RESINS;

PURIFICATION;

ENHANCED GREEN FLUORESCENT PROTEIN; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; RHAMNOSE; SACCHAROMYCES CEREVISIAE PROTEIN;

AFFINITY CHROMATOGRAPHY; ARTICLE; BIOSYNTHESIS; BIOTECHNOLOGY; DRUG EFFECT; ENZYMOLOGY; ESCHERICHIA COLI; GENE VECTOR; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; MOLECULAR BIOLOGY; REPORTER GENE; SACCHAROMYCES CEREVISIAE; TRANSCRIPTION INITIATION;

BIOTECHNOLOGY; CHROMATOGRAPHY, AFFINITY; ESCHERICHIA COLI; GENES, REPORTER; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; MOLECULAR BIOLOGY; RECOMBINANT FUSION PROTEINS; RHAMNOSE; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; TRANSCRIPTIONAL ACTIVATION;

ESCHERICHIA COLI; SACCHAROMYCES CEREVISIAE;

EID: 61449223450     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-008-9909-9     Document Type: Article

References (18)

1. J Altenbuchner R Mattes 2004
2. J Gyuris E Golemis H Chertkov R Brent 1993 Cdi1, a human G1 and S phase protein phosphatase that associates with Cdk2 Cell 75 791 803
3. ES Johnson G Blobel 1999 Cell cycle-regulated attachment of the ubiquitin-related protein SUMO to the yeast septins J Cell Biol 147 981 994
4. SJ Li M Hochstrasser 1999 A new protease required for cell-cycle progression in yeast Nature 398 246 251
5. JJ Lichty JL Malecki HD Agnew DJ Michelson-Horowitz S Tan 2005 Comparison of affinity tags for protein purification Protein Expr Purif 41 98 105
6. SE Luria JN Adams RC Ting 1960 Transduction of lactose-utilizing ability among strains of E. coli and S. dysenteriae and the properties of the transducing phage particles Virology 12 348 390
7. E Mossessova CD Lima 2000 Ulp1-SUMO crystal structure and genetic analysis reveal conserved interactions and a regulatory element essential for cell growth in yeast Mol Cell 5 865 876
8. S Nallamsetty DS Waugh 2006 Solubility-enhancing proteins MBP and NusA play a passive role in the folding of their fusion partners Protein Expr Purif 45 175 182
9. S Nallamsetty DS Waugh 2007 A generic protocol for the expression and purification of recombinant proteins in Escherichia coli using a combinatorial His6-maltose binding protein fusion tag Nat Protoc 2 383 391
10. KD Pryor B Leiting 1997 High-level expression of soluble protein in Escherichia coli using a His6-tag and maltose-binding-protein double-affinity fusion system Protein Expr Purif 10 309 319
11. P Puigbo E Guzman A Romeu S Garcia-Vallve 2007 OPTIMIZER: a web server for optimizing the codon usage of DNA sequences Nucleic Acids Res 35 126 131
12. HM Sassenfeld 2008 Engineering proteins for purification TibTech 8 88 93
13. T Stumpp B Wilms J Altenbuchner 2000 Ein neues L-Rhamnose-induzierbares Expressionssystem für Escherichia coli BIOspektrum 6 33 36
14. K Tanaka J Nishide K Okazaki H Kato O Niwa T Nakagawa H Matsuda M Kawamukai Y Murakami 1999 Characterization of a fission yeast SUMO-1 homologue, pmt3p, required for multiple nuclear events, including the control of telomere length and chromosome segregation Mol Cell Biol 19 8660 8672
15. K Terpe 2003 Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems Appl Microbiol Biotechnol 60 523 533
16. M Uhlen T Moks 1990 Gene fusions for purpose of expression: an introduction Methods Enzymol 185 129 143
17. AC Vertegaal 2007 Small ubiquitin-related modifiers in chains Biochem Soc Trans 35 1422 1423
18. C Yanisch-Perron J Vieira J Messing 1985 Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors Gene 33 103 119