Cold-inducible cloning vectors for low-temperature protein expression in Escherichia coli: Application to the production of a toxic and proteolytically sensitive fusion protein

Gene

Volumn 238, Issue 2, 1999, Pages 325-332

  Mujacic, Mirna ^a   Cooper, Kerri W ^a   Baneyx, François ^a  

^a University of Washington   (United States)

Author keywords

Cold shock; cspA; Degradation; Folding; Proteolysis; Stability; Toxicity

Indexed keywords

BETA LACTAMASE; HYBRID PROTEIN; MEMBRANE PROTEIN; RECOMBINANT PROTEIN;

ARTICLE; CLONING VECTOR; CONTROLLED STUDY; ESCHERICHIA COLI; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN STABILITY; PROTEIN SYNTHESIS;

BACTERIAL PROTEINS; BASE SEQUENCE; BETA-LACTAMASES; CLONING, MOLECULAR; COLD; DNA PRIMERS; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENETIC VECTORS; HYDROLYSIS; PROMOTER REGIONS (GENETICS); RECOMBINANT FUSION PROTEINS; TRANSCRIPTION, GENETIC;

EID: 0032883737     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1119(99)00328-5     Document Type: Article

References (26)

1. Baneyx F. In vivo folding of recombinant proteins in Escherichia coli. Davies J.E., Demain A.L., Cohen G., Hershberger C.L., Forney L.J., Holland I.B., Hu W.S., Wu J.H.D., Sherman D.H., Wilson R.C. Manual of Industrial Microbiology and Biotechnology. 2nd ed. 1999;551-565 American Society for Microbiology, Washington, DC.
2. Baneyx F., Georgiou G. Expression, purification, and enzymatic characterization of a protein A-β-lactamase hybrid protein. Enzyme Microbiol. Technol. 11:1989;559-567.
3. Brandi A., Pietroni P., Gualerzi C.O., Pon C.L. Post-transcriptional regulation of CspA expression in Escherichia coli. Mol. Microbiol. 19:1996;231-240.
4. Derouiche R., Gavioli M., Benedetti H., Prilipov A., Lazdunski C., Lloubes R. TolA central domain interacts with Escherichia coli porins. EMBO J. 15:1996;6408-6415.
5. Fang L., Hou Y., Inouye M. Role of the cold-box region in the 5′ untranslated region of the cspA mRNA in its transient expression at low temperature in Escherichia coli. J. Bacteriol. 180:1998;90-95.
6. Fang L., Jiang W., Bae W., Inouye M. Promoter-independent cold-shock induction of cspA and its derepression at 37°C by mRNA stabilization. Mol. Microbiol. 23:1997;355-364.
7. Ghrayeb J., Kimura H., Takahara M., Hsiung H., Masui Y., Inouye M. Secretion cloning vectors in Escherichia coli. EMBO J. 3:1984;2437-2442.
8. Goldenberg D., Azar I., Oppenheim A.B. Differential mRNA stability of the cspA gene in the cold-shock response of Escherichia coli. Mol. Microbiol. 19:1996;241-248.
9. Goldenberg D., Azar I., Oppenheim A.B., Brandi A., Pon C.L., Gualerzi C.O. Role of Escherichia coli cspA promoter sequences and adaptation of translational apparatus in the cold shock response. Mol. Gen. Genet. 256:1997;282-290.
10. Goldstein J., Pollitt N.S., Inouye M. Major cold shock protein of Escherichia coli. Proc. Natl. Acad. Sci. USA. 87:1990;283-287.
11. Grisshammer R., Tate G.G. Overexpression of integral membrane proteins for structural studies. Q. Rev. Biophys. 28:1995;315-422.
12. Guihard G., Boulanger P., Bénédetti H., Lloubès R., Besnard M., Letellier L. Colicin A and the Tol proteins involved in translocation are preferentially located in the contact sites between the inner and outer membrane of Escherichia coli cells. J. Biol. Chem. 269:1993;5874-5880.
13. Jelsch C., Mourey L., Masson J.M., Samama J.P. Crystal structure of Escherichia coli TEM1 β-lactamase at 1.8 Å resolution. Proteins. 16:1993;364-383.
14. Jiang W., Fang L., Inouye M. The role of the 5′-end untranslated region of the mRNA for CspA, the major cold-shock protein of Escherichia coli, in cold-shock adaptation. J. Bacteriol. 178:1996;4919-4925.
15. Jiang W., Jones P., Inouye M. Chloramphenicol induces the transcription of the major cold shock gene of Escherichia coli cspA. J. Bacteriol. 175:1993;5824-5828.
16. Jones P.G., Inouye M. RbfA, a 30S ribosomal binding factor, is a cold-shock protein whose absence triggers the cold-shock response. Mol. Microbiol. 21:1996;1207-1218.
17. Jones P.G., VanBogelen R.A., Neidhardt F.C. Induction of proteins in response to low temperatures in Escherichia coli. J. Bacteriol. 169:1987;2092-2095.
18. Levengood S.K., Beyer W.F.J., Webster R.E. TolA: a membrane protein involved in colicin uptake contains an extended helical region. Proc. Natl. Acad. Sci. USA. 88:1991;5939-5943.
19. Levengood S.K., Webster R.E. Nucleotide sequence of the tolA and tolB genes and localization of their products components of a multistep translocation system in Escherichia coli. J. Bacteriol. 171:1989;6600-6609.
20. Samuni A. A direct spectrophotometric assay and determination of Michaelis constants for the β-lactamase reaction. Anal. Biochem. 63:1975;17-26.
21. Taura T., Kusukawa N., Yura T., Ito K. Transient shut off of Escherichia coli heat shock protein synthesis upon temperature shift down. Biochem. Biophys. Res. Commun. 163:1989;438-443.
22. Tomoyasu T., Yamanaka K., Murata K., Suzaki T., Bouloc P., Kato A., Niki H., Hiraga S., Ogura T. Topology and subcellular location of FtsH protein in Escherichia coli. J. Bacteriol. 175:1993;1352-1357.
23. Vasina J.A., Baneyx F. Recombinant protein expression at low temperatures under the transcriptional control of the major Escherichia coli cold shock promoter cspA. Appl. Environ. Microbiol. 62:1996;1444-1447.
24. Vasina J.A., Baneyx F. Expression of aggregation-prone recombinant proteins at low temperatures: a comparative study of the Escherichia coli cspA and tac promoter systems. Protein Express. Purif. 9:1997;211-218.
25. Vasina J.A., Peterson M.S., Baneyx F. Scale-up and optimization of the low-temperature inducible cspA promoter system. Biotechnol. Prog. 14:1998;714-721.
26. Webster R.E. The tol gene products and the import of macromolecules into Escherichia coli. Mol. Microbiol. 5:1991;1005-1011.