Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element

Gene

Volumn 192, Issue 2, 1997, Pages 271-281

  Chong, Shaorong ^a   Mersha, Fana B ^a   Comb, Donald G ^a   Scott, Melissa E ^a   Landry, David ^a   Vence, Luis M ^a   Perler, Francine B ^a   Benner, Jack ^a   Kucera, Rebecca B ^a   Hirvonen, Christine A ^a,c   Pelletier, John J ^a   Paulus, Henry ^b   Xu, Ming Qun ^a  

^a NEW ENGLAND BIOLABS   (United States)

^b BOSTON BIOMEDICAL RESEARCH INSTITUTE   (United States)

^c UNIVERSITY OF WISCONSIN MADISON   (United States)

Author keywords

Chitin binding domain; Intein; Protein labeling; Protein purification

Indexed keywords

CYSTEINE; DITHIOTHREITOL; FUNGAL PROTEIN; HYBRID PROTEIN; MERCAPTOETHANOL; RECOMBINANT PROTEIN;

ADSORPTION; AMINO TERMINAL SEQUENCE; ARTICLE; BACILLUS CIRCULANS; CARBOXY TERMINAL SEQUENCE; COLUMN CHROMATOGRAPHY; CONTROLLED STUDY; MOLECULAR CLONING; NONHUMAN; PH; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN DOMAIN; PROTEIN PURIFICATION; SACCHAROMYCES CEREVISIAE; TEMPERATURE;

AFFINITY LABELS; AMINO ACID SEQUENCE; BASE SEQUENCE; CARRIER PROTEINS; CHITIN; GENETIC VECTORS; HYDROGEN-ION CONCENTRATION; METHODS; MOLECULAR SEQUENCE DATA; PROTEIN PROCESSING, POST-TRANSLATIONAL; PROTEIN SPLICING; PROTON-TRANSLOCATING ATPASES; RECOMBINANT PROTEINS; TEMPERATURE; VACUOLAR PROTON-TRANSLOCATING ATPASES;

EID: 17744415288     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1119(97)00105-4     Document Type: Article

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