Escherichia coli expression and purification of LL37 fused to a family III carbohydrate-binding module from Clostridium thermocellum

Protein Expression and Purification

Volumn 71, Issue 1, 2010, Pages 1-7

  Ramos, Reinaldo ^a   Domingues, Lucília ^a   Gama, Miguel ^a  

^a UNIVERSITY OF MINHO   (Portugal)

Author keywords

Antimicrobial peptide; CBM3; Cellulose; Formic acid; LL37

Indexed keywords

ANTIINFECTIVE AGENT; ANTIMICROBIAL CATIONIC PEPTIDE; CAP18 LIPOPOLYSACCHARIDE BINDING PROTEIN; CAP18 LIPOPOLYSACCHARIDE-BINDING PROTEIN; CELL SURFACE RECEPTOR; HYBRID PROTEIN; SACCHARIDE BINDING PROTEINS; SACCHARIDE-BINDING PROTEINS;

ARTICLE; BIOCHEMISTRY; CLOSTRIDIUM THERMOCELLUM; DRUG EFFECT; ESCHERICHIA COLI; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; ISOLATION AND PURIFICATION; MASS SPECTROMETRY; METABOLISM; METHODOLOGY; MICROBIOLOGICAL EXAMINATION; REVERSED PHASE LIQUID CHROMATOGRAPHY;

ANTI-BACTERIAL AGENTS; ANTIMICROBIAL CATIONIC PEPTIDES; BIOCHEMISTRY; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CHROMATOGRAPHY, REVERSE-PHASE; CLOSTRIDIUM THERMOCELLUM; ESCHERICHIA COLI; MICROBIAL SENSITIVITY TESTS; RECEPTORS, CELL SURFACE; RECOMBINANT FUSION PROTEINS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

CLOSTRIDIUM THERMOCELLUM; ESCHERICHIA COLI;

EID: 77049123683     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2009.10.016     Document Type: Article

References (24)

1. Ajesh K., and Sreejith K. Peptide antibiotics: an alternative and effective antimicrobial strategy to circumvent fungal infections. Peptides 30 (2009) 999-1006
2. Hancock R.E., and Diamond G. The role of cationic antimicrobial peptides in innate host defences. Trends Microbiol. 8 (2000) 402-410
3. Powers J.P.S., and Hancock R.E.W. The relationship between peptide structure and antibacterial activity. Peptides 24 (2003) 1681-1691
4. Ramanathan B., Davis E.G., Ross C.R., and Blecha F. Cathelicidins: microbicidal activity, mechanisms of action, and roles in innate immunity. Microbes Infect. 4 (2002) 361-372
5. Durr U.H., Sudheendra U.S., and Ramamoorthy A. LL-37, the only human member of the cathelicidin family of antimicrobial peptides. Biochim. Biophys. Acta 1758 (2006) 1408-1425
6. Niyonsaba F., Iwabuchi K., Someya A., Hirata M., Matsuda H., Ogawa H., and Nagaoka I. A cathelicidin family of human antibacterial peptide LL-37 induces mast cell chemotaxis. Immunology 106 (2002) 20-26
7. Yang D., Chen Q., Schmidt A.P., Anderson G.M., Wang J.M., Wooters J., Oppenheim J.J., and Chertov O. LL-37, the neutrophil granule- and epithelial cell-derived cathelicidin, utilizes formyl peptide receptor-like 1 (FPRL1) as a receptor to chemoattract human peripheral blood neutrophils, monocytes, and T cells. J. Exp. Med. 192 (2000) 1069-1074
8. Heilborn J.D., Nilsson M.F., Kratz G., Weber G., Sorensen O., Borregaard N., and Stahle-Backdahl M. The cathelicidin anti-microbial peptide LL-37 is involved in re-epithelialization of human skin wounds and is lacking in chronic ulcer epithelium. J. Invest. Dermatol. 120 (2003) 379-389
9. Koczulla R., von Degenfeld G., Kupatt C., Krotz F., Zahler S., Gloe T., Issbruicker K., Unterberger P., Zaiou M., Lebherz C., Karl A., Raake P., Pfosser A., Boekstegers P., Welsch U., Hiemstra P.S., Vogelmeier C., Gallo R.L., Clauss M., and Bals R. An angiogenic role for the human peptide antibiotic LL-37/hCAP-18. J. Clin. Invest. 111 (2003) 1665-1672
10. Gilkes N.R., Henrissat B., Kilburn D.G., Miller Jr. R.C., and Warren R.A. Domains in microbial beta-1,4-glycanases: sequence conservation, function, and enzyme families. Microbiol. Rev. 55 (1991) 303-315
11. Tormo J., Lamed R., Chirino A.J., Morag E., Bayer E.A., Shoham Y., and Steitz T.A. Crystal structure of a bacterial family-III cellulose-binding domain: a general mechanism for attachment to cellulose. EMBO J. 15 (1996) 5739-5751
12. Guerreiro C.I., Fontes C.M., Gama M., and Domingues L. Escherichia coli expression and purification of four antimicrobial peptides fused to a family 3 carbohydrate-binding module (CBM) from Clostridium thermocellum. Protein Expr. Purif. 59 (2008) 161-168
13. Waddell W.J. A simple ultraviolet spectrophotometric method for the determination of protein. J. Lab. Clin. Med. 48 (1956) 311-314
14. Vogt T.C.B., and Bechinger B. The interactions of histidine-containing amphipathic helical peptide antibiotics with lipid bilayers - the effects of charges and pH. J. Biol. Chem. 274 (1999) 29115-29121
15. Nishihara K., Kanemori M., Yanagi H., and Yura T. Overexpression of trigger factor prevents aggregation of recombinant proteins in Escherichia coli. Appl. Environ. Microbiol. 66 (2000) 884-889
16. Tomme P., Boraston A., McLean B., Kormos J., Creagh A.L., Sturch K., Gilkes N.R., Haynes C.A., Warren R.A.J., and Kilburn D.G. Characterization and affinity applications of cellulose-binding domains. J. Chromatogr. B Biomed. Sci. Appl. 715 (1998) 283-296
17. Li Y., Li X., and Wang G. Cloning, expression, isotope labeling, and purification of human antimicrobial peptide LL-37 in Escherichia coli for NMR studies. Protein Expr. Purif. 47 (2006) 498-505
18. Yang Y.H., Zheng G.G., Li G., Zhang X.J., Cao Z.Y., Rao Q., and Wu K.F. Expression of bioactive recombinant GSLL-39, a variant of human antimicrobial peptide LL-37 in Escherichia coli. Protein Expr. Purif. 37 (2004) 229-235
19. Moon J.Y., Henzler-Wildman K.A., and Ramamoorthy A. Expression and purification of a recombinant LL-37 from Escherichia coli. Biochim. Biophys. Acta 1758 (2006) 1351-1358
20. Hong I.P., Lee S.J., Kim Y.S., and Choi S.G. Recombinant expression of human cathelicidin (hCAP18/LL-37) in Pichia pastoris. Biotechnol. Lett. 29 (2007) 73-78
21. Giglione C., Boularot A., and Meinnel T. Protein N-terminal methionine excision. Cell. Mol. Life Sci. 61 (2004) 1455-1474
22. Sherman F., Stewart J.W., and Tsunasawa S. Methionine or not methionine at the beginning of a protein. Bioessays 3 (1985) 27-31
23. Li Y., Li X., Li H., Lockridge O., and Wang G. A novel method for purifying recombinant human host defense cathelicidin LL-37 by utilizing its inherent property of aggregation. Protein Expr. Purif. 54 (2007) 157-165
24. Nagaoka I., Hirota S., Niyonsaba F., Hirata M., Adachi Y., Tamura H., Tanaka S., and Heumann D. Augmentation of the lipopolysaccharide-neutralizing activities of human cathelicidin CAP18/LL-37-derived antimicrobial peptides by replacement with hydrophobic and cationic amino acid residues. Clin. Diagn. Lab. Immunol. 9 (2002) 972-982