The FLAG™ peptide, a versatile fusion tag for the purification of recombinant proteins

Journal of Biochemical and Biophysical Methods

Volumn 49, Issue 1-3, 2001, Pages 455-465

  Einhauer, A ^a   Jungbauer, A ^a  

^a UNIVERSITY OF AGRICULTURAL SCIENCES   (Austria)

Author keywords

Chromatography; FLAG ; Recombinant proteins

Indexed keywords

ALKALINE PHOSPHATASE; AMINO ACID; ANTIBODY; CALCIUM ION; CHELATING AGENT; DIVALENT CATION; ENTEROPEPTIDASE; HUMAN SERUM ALBUMIN; HYBRID PROTEIN; INTERLEUKIN 18; INTERLEUKIN 3; INTERLEUKIN 4; LEUKEMIA INHIBITORY FACTOR; METAL ION; MONOCLONAL ANTIBODY; MONOCLONAL ANTIBODY M1; MONOCLONAL ANTIBODY M2; MONOCLONAL ANTIBODY M5; NUCLEAR PROTEIN; NUCLEAR PROTEIN P68; PEPTIDE; RECOMBINANT PROTEIN; TRANSCRIPTION FACTOR; UNCLASSIFIED DRUG; CARRIER PROTEIN; ENZYME; EPITOPE; FLAG PEPTIDE;

DENATURATION; DIAGNOSTIC PROCEDURE; IMMUNOAFFINITY CHROMATOGRAPHY; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DEGRADATION; PROTEIN PURIFICATION; REVIEW; TECHNOLOGY; AFFINITY CHROMATOGRAPHY; BINDING SITE; CHEMISTRY; IMMUNOLOGY; ISOLATION AND PURIFICATION; METHODOLOGY;

ANTIBODIES; BINDING SITES; CARRIER PROTEINS; CHROMATOGRAPHY, AFFINITY; ENZYMES; EPITOPES; PEPTIDES; RECOMBINANT FUSION PROTEINS; RECOMBINANT PROTEINS;

EID: 0035975859     PISSN: 0165022X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0165-022X(01)00213-5     Document Type: Review

References (55)

1. A short polypeptide marker sequence useful for recombinant protein identification and purification
2. One-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase
3. Eukaryotic proteins expressed in Escherichia coli: An improved thrombin cleavage and purification procedure of fusion proteins with glutathione S-transferase
4. Chelating peptide-immobilized metal ion affinity chromatography
5. Fusions to staphylococcal protein A
6. Immobilization and purification of enzymes with staphylococcal protein A gene fusion vectors
7. The cellulose-binding domains of cellulases: Tools for biotechnology
8. Enzyme immobilization using the cellulose-binding domain of a Cellulomonas fimi exoglucanase
9. Vectors that facilitate the expression and purification of foreign peptides in Escherichia coli by fusion to maltose-binding protein
10. Biotination of proteins in vivo; a posttranslational modification to label, purify, and study proteins
11. Multiple epitope tagging of expressed proteins for enhanced detection
12. A calcium-dependent antibody for identification and purification of recombinant proteins
13. A polypeptide fusion designed for the purification of recombinant proteins
14. Genetic approach to facilitate purification of recombinant proteins with a novel metal chelate adsorbent
15. Enzyme purification by genetically attached polycysteine and polyphenylalanine affinity tails
16. Studies on the chemical basis of the antigenicity of proteins
17. Protein surface analysis: Methods for identifying antigenic determinants and other interaction sites
18. Prediction of protein antigenic determinants from amino acid sequences
19. High-level temperature-induced synthesis of an antibody VH-domain in Escherichia coli using the pel B secretion signal
20. Mono- and bivalent antibody fragments produced in Escherichia coli: Engineering, folding and antigen binding
21. Protein expression in yeast; comparison of two expression strategies regarding protein maturation
22. Expression and purification of general transcription factors by Flag epitope tagging and peptide elution
23. Enhancement of membrane insertion and function in a type IIIb membrane protein following introduction of a cleavable signal peptide
24. An improved affinity tag based on the FLAG peptide for the detection and purification of recombinant antibody fragments
25. C5a anaphylatoxin and its seven transmembrane-segment receptor, A
26. Specificity of the thrombin receptor for agonist peptide is defined by its extracellular surface
27. Heterogeneity in human interleukin-3 receptors; a subclass that binds human granulocyte/macrophage clony stimulating factor
28. Metal-binding properties of a calcium-dependent monoclonal antibody
29. Mapping the fine specificity of a calcium-dependent monoclonal antibody through the use of soluble positional scanning combinatorial libraries: Identification of potent calcium independent antigens
30. Immunoaffinity purification of FLAG epitope-tagged bacterial alkaline phospatase using a novel monoclonal antibody and peptide elution
31. The p50 subunit of NF-kB associates with the NF-IL6 transcription factor
32. Vectors for expression and secretion of FLAG epitope-tagged proteins in mammalian cells
33. Short cut of protein purification by integration of cell-disrupture and affinity extraction
34. Generation of β-globin by sequence-specific proteolysis of a hybrid protein in Escherichia coli
35. Sequence and immunogenicity of the 70-kDA heat shock protein of Mycobacterium leprae
36. Biochemical and mutational analysis of a plant virus polyprotein cleavage site
37. Cloning and functional expression of a cDNA encoding the catalytic subunit of bovine enterokinase
38. Production of recombinant bovine enterokinase catalytic subunit in Escherichia coli using the novel secretory fusion partner DsbA
39. One-step purification of hybrid proteins which have β-galactosidase activity
40. The isolation and characterization of human atrial natriuretic factor produced as a fusion protein in Escherichia coli
41. Gene synthesis, expression in Escherichia coli and purification of immunoreactive human insulin-like growth factors I and II: Application of a modified HPLC separation technique for hydrophobic proteins
42. Expression of human insulin-like growth factor I in bacteria: Use of optimized gene fusion vectors to facilitate protein purification
43. Export and purification of a cytoplasmic dimeric protein by fusion to the maltose-binding protein of Escherichia coli
44. Production in Escherichia coli and one-step purification of bifunctional hybrid proteins which bind maltose. Export of the Klenow polymerase into the periplasmic space
45. Adsorption to starch of a β-galactosidase fusion protein containing the starch-binding region of Aspergillus glucoamylase
46. Purification of recA-based fusion proteins by immunoadsorbent chromatography: Characterization of a major antigenic determinant of Escherichia coli recA protein
47. The purification of recombinant proteins using C-terminal polyarginine fusions
48. Polyelectrolyte precipitation of β-galactosidase fusions containing poly-aspartic acid tails
49. A novel enzymatic method for production of authentic hGH from an Escherichia coli produced hGH-precursor
50. Baculoviral transfer vectors for expression of FLAG fusion proteins in insect cells
51. Limitations for purification of murine interleukin-18 when expressed as a fusion protein containing the FLAG™ peptide
52. A polar octapeptide fused to the N-terminal fusion peptide solubilizes the influenza virus HA2 subunit ectodomain
53. Efficient site specific removal of a C-terminal FLAG fusion from a Fab′ using copper(II) ion catalysed protein cleavage